Effect of micro-aerobic conditions based on semipermeable membrane-covered on greenhouse gas emissions and bacterial community during dairy manure storage at industrial scale

Chen, Fang; Zhou, Lei; Liu, Ya; Xiong, Jinpeng; Su, Ya; Lan, Zefeng; Han, Lujia; Huang, Guangqun

doi:10.1016/j.envpol.2022.118879

Cited by 28 publications

(7 citation statements)

References 46 publications

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“…From the phylum level, Firmicutes , Actinobacteria , Bacteroidetes , Proteobacteria , Chloroflexi , Spirochaetae , Tenericutes , and Deinococcus-Thermus were found to be predominant in dairy manure composting, accounting for over 90% of community abundance in each sample. Fang et al [ 12 ] also found these phyla in the dairy manure large-scale aerobic composting. Wang et al [ 46 ] indicated that Firmicutes , Bacteroidetes , Actinobacteria , and Proteobacteria accounted for a larger proportion of the community and were influenced by physicochemical parameters.…”

Section: Resultsmentioning

confidence: 95%

“…The organic matter (OM) was determined by using the standard method TMECC 05.07A. The carbon/nitrogen ratio was determined by an elemental analyzer (Elementar Vario ELIII, Augsburg, Germany) [ 12 ]. The determination of pH and electrical conductivity (EC) was based on TMECC 04.11A.…”

Section: Methodsmentioning

confidence: 99%

“…Ma et al [ 9 ] demonstrated that functional membranes decreased the possibility of global warming by 16.97% and improved the relative abundance of Actinobacteria . Fang et al [ 12 ] suggested that carbon dioxide (CO 2 ) and methane (CH 4 ) were reduced by 78.68% and 99.97%, respectively, in the membrane-covered group compared with the control group. Fang et al [ 13 ] found that membrane-covered composting improved the oxygen utilization rate and inhibited the anaerobic bacterial genus Hydrogenispora and archaea order Methanobacteriales .…”

Section: Introductionmentioning

confidence: 99%

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Large Semi-Membrane Covered Composting System Improves the Spatial Homogeneity and Efficiency of Fermentation

Sun

Huang

et al. 2022

IJERPH

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Homogenous spatial distribution of fermentation characteristics, local anaerobic conditions, and large amounts of greenhouse gas (GHGs) emissions are common problems in large-scale aerobic composting systems. The aim of this study was to examine the effects of a semi-membrane covering on the spatial homogeneity and efficiency of fermentation in aerobic composting systems. In the covered group, the pile was covered with a semi-membrane, while in the non-covered group (control group), the pile was uncovered. The covered group entered the high-temperature period earlier and the spatial gradient difference in the group was smaller compared with the non-covered group. The moisture content loss ratio (5.91%) in the covered group was slower than that in the non-covered group (10.78%), and the covered group had a more homogeneous spatial distribution of water. The degradation rate of organic matter in the non-covered group (11.39%) was faster than that in the covered group (10.21%). The final germination index in the covered group (85.82%) was higher than that of the non-covered group (82.79%) and the spatial gradient difference in the covered group was smaller. Compared with the non-covered group, the oxygen consumption rate in the covered group was higher. The GHG emissions (by 30.36%) and power consumption in the covered group were reduced more significantly. The spatial microbial diversity of the non-covered group was greater compared with the covered group. This work shows that aerobic compost covered with a semi-membrane can improve the space homogeneity and efficiency of fermentation.

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Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Large Semi-Membrane Covered Composting System Improves the Spatial Homogeneity and Efficiency of Fermentation

Sun

Huang

et al. 2022

IJERPH

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“…In the cooling phase of aerobic fermentation, its relative abundance increased with decreasing temperature. At the same time, Bacteroidetes are considered specialists in degrading high molecular weight compounds [30] , and another research report also pointed out that in the cooling phase, Bacteroidetes became the dominant bacteria to degrade cellulose and hydrolyze polysaccharides [15] . Therefore, high molecular weight organic matter that was not completely degraded in the early phase and the hydrolyzed polysaccharide produced by the decomposition of organic matter in the fermentation process may have been degraded by Bacteroidetes in the cooling phase.…”

Section: Analysis Of Bacterial Community Composition At Phylum Levelmentioning

confidence: 99%

Succession of bacteria communities during production and application of dairy bedding by membrane-covered aerobic fermentation�

Yin,

Zeng,

Fang

et al. 2024

International Journal of Agricultural and Biological Engineering

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The cost of dairy manure treatment and bedding material purchase increases the operating cost of the dairy farm. Membrane-covered aerobic fermentation system has been widely used for dealing with dairy manure and recycling the final product as bedding material. However, the microbial safety in each processing step is still uncertain. To better understand the bacterial community dynamics during the whole bedding conversion process, a full-chain and large-scale experiment including 16-day membrane-covered aerobic fermentation and 11-day bedding material application was conducted. The results showed that the pile temperatures in the fermentation stage rapidly increased to 80°C and maintained >50°C for more than 11 d and the use of fermentation product as bedding material provided cows with a stable and comfortable bedding environment. The Chao1 and Shannon index decreased at the end of the fermentation stage and remained stable in the application stage, indicating that membrane-covered aerobic fermentation effectively killed some pathogenic bacteria and guaranteed both the maturity and stability of the final product. The dominant bacteria in the fermentation stage were Acinetobacter, Thermus, and Rhodothermus at genus level. Seven common potential pathogens of mastitis (Staphylococcus, Enterococcus, Serratia, Pseudomonas, Corynebacterium, Mycobacterium, and Bacillus) were found at the end of fermentation stage but the relative abundance was low (0.0025%-0.2727%). The dominant bacteria in the application stage mainly included Acinetobacter, Pseudomonas, and Flavobacterium at the genus level. The relative abundance of Pseudomonas increased in the application stage, which was a reminder to the dairy farm to pay attention to the disinfection and timely replacement of bedding material to prevent the occurrence of dairy mastitis. The results of this study contributed deep understanding of the microorganism-driven bedding conversion process and provide practical guidance and cautions for the bedding materials application.

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“…Since then, a series of metataxonomic studies have reported the presence of MBA03 in anaerobic digester systems (Dyksma et al, 2020;FitzGerald, 2018;Laguillaumie et al, 2022), representing up to 70% of the relative abundance in some samples (FitzGerald, 2018). This taxon has been associated with a higher production of methane and has been suggested as an indicator of the stability of the AD process (Fang et al, 2022;Laguillaumie et al, 2022). Surprisingly, MBA03 has not been properly taxonomically delimited and no culturable specimen or genome of this taxon has ever been reported.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the ecology, taxonomy and metabolic capabilities of MBA03, a potential key player in anaerobic digestion

Puchol-Royo,

Pascual,

Ortega-Legarreta

et al. 2023

Preprint

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Biogas, a mix of CO2, CH4 and small proportions of other gases, is a biofuel obtained by anaerobic digestion (AD). Biogas production is often considered a black box process, as the role and dynamics of some of the microorganisms involved remain undisclosed. Previous metataxonomic studies in the frame of the MICRO4BIOGAS project (www.micro4biogas.eu) revealed that MBA03, an uncharacterised and uncultured bacterial taxon, was very prevalent and abundant in industrial full-scale AD plants. Surprisingly, no culturable specimen or genome of this taxon has ever been reported, so its role in AD has remained unclear. In the present work, thirty samples derived from anaerobic digesters were sequenced, allowing the reconstruction of 108 metagenome-assembled genomes (MAGs) potentially belonging to MBA03. According to phylogenetic analyses and genomic similarity indices, MBA03 constitutes a new bacterial order, proposed as Darwinibacteriales ord. nov., which includes Darwinibacter acetoxidans gen. nov., sp. nov. of the family Darwinibacteriaceae fam. nov., along with Wallacebacter cryptica gen. nov., sp. nov. of the Wallacebacteriaceae fam. nov. Ecotaxonomic studies determined that AD processes are the main ecological niche of Darwinibacteriales. Moreover, metabolic predictions identified Darwinibacteraceae members as putative syntrophic acetate oxidising bacteria (SAOB), as they encode for the reversed Wood-Ljungdahl pathway coupled to the glycine cleavage system. This suggests that Darwinibacteraceae members work in collaboration with hydrogenotrophic archaea to produce methane in industrial biogas plants. Overall, our findings present Darwinibacteriales as a potential key player in anaerobic digestion and pave the way towards the complete characterisation of this newly described bacterial taxa.

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Effect of micro-aerobic conditions based on semipermeable membrane-covered on greenhouse gas emissions and bacterial community during dairy manure storage at industrial scale

Cited by 28 publications

References 46 publications

Large Semi-Membrane Covered Composting System Improves the Spatial Homogeneity and Efficiency of Fermentation

Large Semi-Membrane Covered Composting System Improves the Spatial Homogeneity and Efficiency of Fermentation

Succession of bacteria communities during production and application of dairy bedding by membrane-covered aerobic fermentation�

Unveiling the ecology, taxonomy and metabolic capabilities of MBA03, a potential key player in anaerobic digestion

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