Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Jia, Bin; Li, Yutao; Yang, Bin; Xiao, Di; Zhang, Shengnan; Rajulu, A. Varada; Kondo, Tetsuo; Zhang, Lina; Zhou, Jinping

doi:10.1007/s10570-013-9952-0

Cited by 55 publications

(35 citation statements)

References 48 publications

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“…The potential application of microcrystalline cellulose and CNCs as fillers in electrospun CA for vascular tissue scaffolds has been investigated 171 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 direction, having a positive effect on creating a more uniform morphology (diameters ranging from 212 nm to 221 nm). This ordered microstructure combined with the strong interfacial bonding between CNCs and the regenerated cellulose matrix, remarkably improved the tensile properties of non-woven mats (tensile strength and elastic modulus increased by 101.7 % and 171.6 %, respectively, in the fiber alignment direction, with 20 % loading of CNCs).…”

Section: Nano and Microfibers Matsmentioning

confidence: 99%

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

2014

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Cellulose nanocrystals (CNCs) are a renewable nanosized raw material that is drawing a tremendous level of attention from the materials community. These rod-shaped nanocrystals that can be produced from a variety of highly available and renewable cellulose-rich sources are endowed with exceptional physicochemical properties which have promoted their intensive exploration as building blocks for the design of a broad range of new materials in the past few decades. However, only recently have these nanosized substrates been considered for bioapplications following the knowledge on their low toxicity and ecotoxicological risk. This Review provides an overview on the recent developments on CNC-based functional biomaterials with potential for tissue engineering (TE) applications, focusing on nanocomposites obtained through different processing technologies usually employed in the fabrication of TE scaffolds into various formats, namely, dense films and membranes, hierarchical three-dimensional (3D) porous constructs (micro/nanofibers mats, foams and sponges), and hydrogels. Finally, while highlighting the major achievements and potential of the reviewed work on cellulose nanocrystals, alternative applications for some of the developed materials are provided, and topics for future research to extend the use of CNCs-based materials in the scope of the TE field are identified.

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Section: Nano and Microfibers Matsmentioning

confidence: 99%

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

2014

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“…Jia et al 81 reported potential application of microcrystal cellulose (MCC) and CNC as a ller of electrospun cellulose acetate vascular tissue scaffolds to improve biocompatibility. Thus, although the present work should be biocompatible, given that both components have been previously tested separately, future investigations are needed to characterize the toxicology of these cellulose nanocomposites materials thorough in vitro and in vivo testing, to conrm the potential biomedical application.…”

Section: -79mentioning

confidence: 99%

Mechanical properties and degradation studies of poly(mannitol sebacate)/cellulose nanocrystals nanocomposites

et al. 2015

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A., 2008, 105, 2307). However, PPS generally display poor mechanical properties, in particular a low modulus, that limit the true potential of these materials in the biomedical field. Here, we introduce an approach to obtain nanocomposites based on poly(mannitol sebacate) (PMS) matrices reinforced with cellulose nanocrystals (CNCs) in order to improve the application range of these materials. Different strategies were used based on varying the feed ratios between mannitol : sebacic acid (1 : 1 and 1 : 2), crosslinking conditions and CNCs content, resulting in different degrees of crosslinking and, therefore, mechanical and degradation behavior. All of the developed nanocomposites displayed the expected mass loss during the degradation studies in simulated body fluid (SBF) similar to the neat matrix, however, doubling the sebacic acid feed ratio or extending the curing temperature and time, resulted in higher mechanical properties, structural integrity, and shape stability during a degradation time lessening mass loss rate. Changing mannitol : sebacic acid reaction ratios from 1 : 1 to 1 : 2 and for low crosslinking degree neat samples, the Young's modulus increases four-fold, while mass loss after 150 days of incubation is reduced by half. The Young's modulus range obtained with this process covers the range of human elastic soft tissues to tough tissues (0.7-200 MPa). IntroductionIn the past few years, there has been steady progress in the development of biodegradable polymers for application in the medical eld because these materials provide new opportunities to design less invasive and resorbable implants, tissue scaffolds, and medical devices that are able to avoid second revision surgeries, that limit the risk of infection and results in less trauma for the patient.5-8 Moreover, biodegradable polymeric systems which offer the possibility to tailor their mechanical properties from so to stiffer as well as the biodegradability ratio by varying the processing conditions also have additional advantages. Medical devices based on different classes of biodegradable polymers such as polyesters, polyanhydrides and polyurethanes have been widely investigated and some of them are commercially available as resorbable sutures, drug delivery systems, vascular gras, wafers and orthopaedic xation devices based, among others, on polylactic acid (PLA), polyglycolic acid (PGA), poly(lactic-co-glycolic acid) (PLGA), poly(3-caprolactone) (PCL), poly(p-dioxanone) (PDO), or poly(trimethylene carbonate) (PTMC).9,10 However, most of these polymeric systems have degradation rates that are not optimal for short-medium term applications. For example, it takes more than 24 months for poly(caprolactones) and poly(Llactic acid) to degrade in a biological environment, and from 2 to 24 months, depending on the ratio, for copolymers of polylactic acid (PLA) and polyglycolic acid (PGA). Also, the degradation rate for polyanhydrides is slow, around 12 months. 6,9,11 Moreover, these polymers have a lack of exibility and elasticity at bod...

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“…CNFs have been investigated as a promising substrate for regenerative medicine and wound healing such as scaffolds for tissue-engineered meniscus, blood vessels, and ligament or tendon substitutes (Mathew et al 2011(Mathew et al , 2012Jia et al 2013;Mathew et al 2013;Lin and Dufresne 2014). The applicability of CNFs as hemodialysis membranes (Ferraz et al 2012(Ferraz et al , 2013, antimicrobial nanomaterials (Martins et al 2012;Liu et al 2014) and in the pharmaceutical industry as films for long-lasting sustained drug delivery (Valo et al 2011;Kolakovic et al 2012) has also been documented.…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

et al. 2014

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Cellulose nanofibrils (CNFs), unique and promising natural materials have gained significant attention recently for biomedical applications, due to their special biomechanical characteristics, surface chemistry, good biocompatibility and low toxicity. However, their long bio-persistence in the organism may provoke immune reactions and this aspect of CNFs has not been studied to date. Therefore, the aim of this work was to examine and compare the cytocompatibility and immunomodulatory properties of CNFs in vitro. CNFs (diameters of 10-70 nm; lengths of a few microns) were prepared from Norway spruce (Picea abies) by mechanical fibrillation and high pressure homogenisation. L929 cells, rat thymocytes or human peripheral blood mononuclear cells (PBMNCs) were cultivated with CNFs. None of the six concentrations of CNFs (31.25 µg/ml-1 mg/ml) induced cytotoxicity and oxidative stress in the L929 cells, nor induced necrosis and apoptosis of thymocytes and PBMNCs. Higher concentrations (250 µg/ml-1 mg/ml) slightly inhibited the metabolic activities of the L929 cells as a consequence of inhibited proliferation. The same concentrations of CNFs suppressed the proliferation of PBMNCs to phytohemaglutinine, a T-cell mitogen, and the process was followed by down-regulation of interleukin-2 (IL-2) and interferon-γ production. The highest concentration of CNFs inhibited IL-17A, but increased IL-10 and IL-6 production. The secretion of pro-inflammatory cytokines, IL-1β and tumor necrosis factor-α as well as Th2 cytokine (IL-4), remained unaltered. In conclusion, the results suggest that these CNFs are cytocompatible nanomaterial, according to current ISO criteria, with non-inflammatory and nonimmunogenic properties. Higher concentrations seem to be tolerogenic to the immune system, a characteristic very desirable for implantable biomaterials.

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Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Cited by 55 publications

References 48 publications

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

Mechanical properties and degradation studies of poly(mannitol sebacate)/cellulose nanocrystals nanocomposites

Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

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