Effect of MicroRNA-20a on Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells

Luo, Tao; Yang, Xueqin; Sun, Yan; Huang, Xinqi; Zou, Ling; Liu, Jun

doi:10.1159/000506304

Cited by 11 publications

(12 citation statements)

References 35 publications

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“…For instance, miR-20a-5p is involved in myoblast differentiation [ 17 ], pulmonary surfactant gene expression [ 18 ], angiogenesis [ 19 ], immunomodulation [ 20 ], and tumorigenesis [ 21 – 23 ]. Regarding the roles of miR-20a-5p in the bone metabolism, previous studies show miR-20a-5p could promote osteogenic differentiation of hMSCs and human adipose-derived stem cells (hASCs) [ 24 , 25 ], and miR-20a-5p could target PPARγ to regulate osteoclastogenesis of THP-1 cells [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38

et al. 2021

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Background Human dental pulp stem cells (hDPSCs) are the preferable choice of seed cells for craniomaxillofacial bone tissue regeneration. As a member of the miR-17-92 cluster, miR-20a-5p functions as an important regulator during bone remodeling. This study aimed to investigate the roles and mechanisms of miR-20a-5p during osteogenesis of hDPSCs. Methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was conducted to determine the expression of miR-20a-5p during osteogenesis of hDPSCs. We interfered with the expression of miR-20a-5p in hDPSCs to clarify the function of miR-20a-5p on osteogenesis both in vitro and vivo. Direct bind sites between miR-20a-5p and BAMBI were confirmed by dual-luciferase reporter assay, and the underlying mechanisms were investigated with cell co-transfections. Results The expression of miR-20a-5p was showed to be upregulated during osteogenesis of hDPSCs. Inhibition of miR-20a-5p could weaken the intensity of ALP/ARS staining and downregulate the expression of mRNAs and proteins of osteogenic markers, while overexpression of miR-20a-5p could enhance the intensity of ALP/ARS staining and the expression of osteogenic markers. Both micro-CT reconstruction images and histological results showed that miR-20a-5p could promote the regeneration of calvarial defects. miR-20a-5p directly targeted bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI), and the latter one was an inhibitor of hDPSC osteogenesis. Silencing BAMBI partially reversed the suppression effect of miR-20a-5p knockdown on osteogenesis. Phosphorylation of Smad5 and p38 was decreased when miR-20a-5p was silenced, whereas p-Smad5 and p-p38 were upregulated when miR-20a-5p was overexpressed or BAMBI was silenced. Conclusions It is demonstrated that miR-20a-5p functioned as a regulator of BAMBI to activate the phosphorylation of Smad5 and p38 during osteogenic differentiation of hDPSCs.

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Section: Introductionmentioning

confidence: 99%

miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38

et al. 2021

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“…Therefore, certain miRNAs as positive or negative regulators have been introduced in the osteogenic differentiation of hAMSCs. 29 …”

Section: Introductionmentioning

confidence: 99%

3D-Printed PCL Scaffolds Coated with Nanobioceramics Enhance Osteogenic Differentiation of Stem Cells

et al. 2021

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With advances in bone tissue engineering, various materials and methods have been explored to find a better scaffold that can help in improving bone growth and regeneration. Three-dimensional (3D) printing by fused deposition modeling can produce customized scaffolds from biodegradable polyesters such as polycaprolactone (PCL). Although the fabricated PCL scaffolds exhibited a lack of bioactivity and poor cell attachment on their surfaces, herein, using a simple postfabrication modification method with hydroxyapatite (HA) and bioglasses (BGs), we obtained better cell proliferation and attachment. Biological behavior and osteosupportive capacity of the 3D-printed scaffolds including PCL, PCL/HA, PCL/BG, and PCL/HA/BG were evaluated in this study, while human adipose tissue-derived mesenchymal stem cells (hADSCs) were cultured on the scaffolds. The cell morphology, attachment, and proliferation were investigated using scanning electron microscopy (SEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and 4′,6-diamidino-2-phenylindole (DAPI) staining. In the next step, the ability of stem cells to differentiate into osteoblasts was evaluated by measuring alkaline phosphatase (ALP) activity, calcium deposition, and bone-related gene and protein expression. In the end, the expression levels of miR-20a, miR-125a, and their target genes were also investigated as positive and negative regulators in osteogenesis pathways. The results showed that the coated scaffolds with bioceramics present a more appropriate surface for cell adhesion and proliferation, as well as efficient potential in inducing osteoconduction and osteointegration compared to PCL alone and control. The PCL/HA/BG scaffold exhibited higher in vitro cell viability and bone formation compared to the other groups, which can be due to the synergistic effect of HA and BG. On the whole, this tricomponent 3D-printing scaffold has a promising prospect for bone tissue engineering applications.

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“…miR-20a also plays a role in osteogenic differentiation of human mesenchymal stem cells (MSCs) through the BMP2/Runx2 signaling pathway by targeting Crim1, PPARγ and Bambi [16]. In addition, upregulation of miR-20a promoted osteogenic differentiation of human adipose tissue-derived stem cells (hASCs) [44]. Moreover, a recent study showed that miR20a negatively regulates THP-1 cell proliferation as well as osteoclastogenesis during osteoclast differentiation by down-regulating PPARγ [45].…”

Section: Discussionmentioning

confidence: 99%

MiR-20a: a mechanosensitive microRNA Regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by target BAMBI and SMAD6

Hong

Peng

Mai

et al. 2022

Preprint

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Objective: MicroRNAs (miRNAs) are crucial regulators of diverse biological and pathological process. This study aimed to investigate the role of miR-20a in fluid shear stress(FSS)-mediated osteogenic differentiation.Methods: In the present study, we subject osteoblast MC3T3-E1 cells or mouse bone marrow stromal cells(BMSCs) to single bout short duration fluid shear stress(12 dyn/cm2 for 1 hour) by using a parallel plate flow system. The expression of miR-20a was quantified with miRNA array profiling and quantitative RT-PCR (qRT-PCR) during the FSS-mediated osteogenic differentiation. Bioinformatics analysis and a luciferase assay were performed to confirm potential targets of miR-20a.Results: Osteoblast-expressed miR-20a is sensitive to the mechanical environments of FSS, which is differentially upregulated During Steady FSS-mediated Osteogenic Differentiation. MiR-20a enhances FSS-induced osteoblast differentiation by activating the BMP2 signaling pathway. Both BAMBI and SMAD6 are targets of miR-20a that negatively regulate the BMP2 signaling pathway.Conclusion: MiR-20a is a novel mechano-sensitive miRNA that can enhance osteoblast differentiation in FFS mechanical environments, implying that this miRNA might be a target for bone tissue engineering and orthodontic bone remodeling for regenerative medicine applications.

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Effect of MicroRNA-20a on Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells

Cited by 11 publications

References 35 publications

miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38

miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38

3D-Printed PCL Scaffolds Coated with Nanobioceramics Enhance Osteogenic Differentiation of Stem Cells

MiR-20a: a mechanosensitive microRNA Regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by target BAMBI and SMAD6

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