This study explores the comparative fatigue performance of decarburized surfaces in railway components, emphasizing rolling contact fatigue, crack propagation, and acoustic emission. The investigation entails the examination of two grades of railway steels, namely R260 and U71Mn, to analyze crack and surface characteristics subsequent to fatigue testing employing a Twin Roller Machine. The purpose is to discern the impact of decarburization on the fatigue life of these materials. The results reveal distinct patterns in crack propagation and acoustic emission between decarburized and non-decarburized surfaces, providing valuable insights into the fatigue behavior of railway components. This comparative analysis contributes to a nuanced understanding of the material’s response to cyclic loading.