Effect of Microtubule Inhibitors on Invasion and on Related Activities of Tumor Cells

Mareel, Marc; Mets, M. De

doi:10.1016/s0074-7696(08)61489-8

Cited by 76 publications

(27 citation statements)

References 154 publications

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“…I. Furusawa, Kyoto University. Table I shows the effects of microtubule inhibitors, colchicine and VB (14), and microfilament inhibitors, CB and phalloidin (9, 19) on electrofusion. In this experiment, colchicine induced slight increase in the percentage of broken fusion products with decreasing spherical fusion.…”

Section: Methodsmentioning

confidence: 99%

Possible Involvement of Calmodulin and the Cytoskeleton in Electrofusion of Plant Protoplasts

Abe

Takeda²

1986

Plant Physiol.

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Section: Methodsmentioning

confidence: 99%

Possible Involvement of Calmodulin and the Cytoskeleton in Electrofusion of Plant Protoplasts

Abe

Takeda²

1986

Plant Physiol.

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“…Mzgration means translocation over a longer distance so that the displacement can be assessed without determination of the geometric center of the cell. Directional migration points to migration that occurs preferentially in a certain direction and that is measured in that direction, regardless the cause of directionality (13). Shape change is any change in the shape of the horizontal projection of the cell on the substrate.…”

Section: Cell Motility Terminologymentioning

confidence: 99%

“…Various aspects of cell motility (see Methods for terminology used in this paper) can be assessed in vitro. Several methods have been described to quantify translocation (7,15,23), directional migration including chemokinesis and chemotaxis (13,251, as well as shape change (17,24). Quantification of fast plasma membrane movements involved in ruffling, blebbing, formation or movement of cytopodia, and fast shape change or fast translocation has received less attention.…”

mentioning

confidence: 99%

Simple method for quantification of fast plasma membrane movements

1992

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We present a method for the quantification of the fast plasma membrane movements that are involved in ruffling, blebbing, fast shape change, and fast translocation. The method is based on the Kontron Vidas image analysis computer program. Video images from cells viewed through an inverted microscope were transmitted to the computer. The procedure was as follows: 4 consecutive video images were averaged (imagel); 28 s later a second set of 4 video images was averaged (image 2); image 2 was subtracted from image 1 and the grey level of each pixel of the resulting image was increased with 128 grey level units, resulting in the subtraction image, showing a uniform grey background speckled with brighter and darker spots corresponding to areas of movement. These spots were discriminated and turned into white objects against a black background. Interactive editing was used to delete artefacts that resulted from floating debris. The total area of the discriminated objects was measured, and the parameter motile area in pm2 per cell was calculated. We have applied our method to the study of motility induced in epithelial cell lines by the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate and by epidermal growth factor.Key terms: Cell motility, cell membrane, cell-surface, ruffling, blebbing, cellshape, translocation, image analysis, TPA, EGF Direct analysis of cell motility in vivo is problematic because of the lack of transparency of three-dimensional living tissues. There is evidence indicating that the study of cell motility on a flat surface in vitro is relevant for the situation in vivo because cells have an inherent motility program (11,12,21). Various aspects of cell motility (see Methods for terminology used in this paper) can be assessed in vitro. Several methods have been described to quantify translocation (7,15,23), directional migration including chemokinesis and chemotaxis (13,251, as well as shape change (17,24). Quantification of fast plasma membrane movements involved in ruffling, blebbing, formation or movement of cytopodia, and fast shape change or fast translocation has received less attention. Partin et al. (17) have described an elaborate Fourier analysis for quantification of the various aspects of cell motility, including ruffling. Recently Tatsuka et al. (22) described a method for quantification of "momentary alterations of cell shape" reflecting ruff ling, pseudopodal activity, and fast cell translocation, using automatic image analysis on Allen video-enhanced contrast-differential interference contrast images. Here, we report on a simple and rapid method for quantification of fast plasma membrane movements. It is entirely based on the Vidas automatic grey level image analysis program that is commercially available from Kontron (Eching, West Germany). We have tested our method on cell cultures differing in their type or intensity of motility as estimated from time-lapse video films. As an application of our method we analysed the effect of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acet...

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“…Table 1 shows that growth of H . halobium was inhibited by antitumor drugs which interact in eucaryotes with tubulin (vincristine, nocodazole, podophyllotoxin [12] or type I1 DNA topoisomerases (two anthracyclines, adriamycin and daunorubicin and two semi-synthetic epipodophyllotoxins, VP16 and VM26) [6, 131. In addition, H .…”

Section: Inhibition Of Halophilic Archaebacteriul Growth By Antitumormentioning

confidence: 99%

Antitumor drugs inhibit the growth of halophilic archaebacteria

et al. 1987

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Permeability mutants of Escherichia coli have been used to prescreen antitumor drugs. However, most compounds active against eucaryotic proteins have no effect on isofunctional proteins of eubacteria. In contrast, we show that growth of halophilic archaebacteria, procaryotes as distantly related to eubacteria as to eucaryotes, is inhibited by several drugs known to interact with tubulin, actomyosin and DNA topoisomerase I1 of eucaryotes. Actually, different types of evidence indicate the presence of analogous proteins in halophilic archaebacteria : (a) a yeast actin probe hybridizes with DNA restriction digests of Halobacterium halobium; (b) antibodies against tubulin and actin from chicken react in a crude extract of H. halohiurn with polypeptides having M , of 55000 and 80000, respectively; (c) the epipodophyllotoxin VP16, a eucaryotic DNA topoisomerase I1 inhibitor, induces DNA strand breaks with DNA-protein covalent linkage in H . halohiurn as in eucaryotes. Besides the evolutionary implications, these data indicate that halophilic archaebacteria can be used to prescreen antitumor drugs active on eucaryotic proteins.Antibiotics useful in cancer therapy include drugs whose primary target is DNA (alkylating agents, DNA intercalators, DNA binding agents) and drugs which interact with essential cellular proteins (tubulin, type I1 DNA topoisomerases). Procaryotic microorganisms, especially the eubacterium Escherichia coli, have been useful for prescreening a few antitumor drugs [l]. However, most antibiotics active against eucaryotic proteins have no effect on isofunctional proteins in eubacteria; therefore, these procaryotes are only suitable to search for drugs which interact directly with DNA. Unfortunately, such compounds are not only carcinostatic but also carcinogenic [l]. Archaebacteria constitute a group of procaryotes as distantly related to classical bacteria (eubacteria) as to eucaryotes [2]. Growth of some archaebacteria is inhibited by anisomycin and aphidicolin, two antibiotics which were hitherto known to be specific for eukaryotic cells [3, 41. Recently, we have shown that growth of halophilic archaebacteria (halobacteria) is inhibited by the epipodophyllotoxin etoposide (VP16) [5], an inhibitor of eucaryotic type I1 DNA topoisomerases which is widely used in tumor therapy [6]. This prompted us to check whether halobacteria were sensitive to other antitumor drugs. Halobacteria are particularly suitable for in vivo studies with Abbreviations. m-AMSA, 4-(9-acridinylamino)methane sulfonm-aniside; 2-Me-90H-E, 2-methyl-9-hydroxyellipticinium; NaCl/Cit, 0.15 M NaCI, 0.015 M trisodium citrate, pH 7.0; VM26, 4 -demethylepipodophyllotoxin thenylidene fl-D-ghcoside; VP16, demethylepipodophyllotoxin ethylidene fl-D-glucoside; ID,o, drug concentration inhibiting cell growth by 50%.

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Effect of Microtubule Inhibitors on Invasion and on Related Activities of Tumor Cells

Cited by 76 publications

References 154 publications

Possible Involvement of Calmodulin and the Cytoskeleton in Electrofusion of Plant Protoplasts

Possible Involvement of Calmodulin and the Cytoskeleton in Electrofusion of Plant Protoplasts

Simple method for quantification of fast plasma membrane movements

Antitumor drugs inhibit the growth of halophilic archaebacteria

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