Effect of mild-intensity exercise training with capsiate intake on fat deposition and substrate utilization during exercise in diet-induced obese mice

Abstract

While the anti-obesity effects of exercise and capsiate are well-observed individually, the effect of exercise with capsiate intake has not been systematically explored yet. Therefore, the purpose of this study is to investigate whether the anti-obesity effects of exercise training can be further enhanced by capsiate intake. [Methods] 8-week-old male mice were divided into 3 groups (n = 8 per group): sedentary group (SED; nontrained), exercise-trained group (EXE) and exercisetrained group with 10 mg/kg of capsiate intake (EXE+CAP). All mice were offered high-fat diet and water ad libitum. The mild-intensity treadmill training was conducted 5 times a week for 8 weeks. After 8 weeks, metabolism during exercise and abdominal fat weight were measured. [Results] Body weight and the rate of total abdominal fat were significantly less in EXE+CAP than in SED but not between EXE and SED. The average of respiratory exchange rate during exercise was significantly much lower in EXE+SED (p = 0.003) compared to the difference between EXE and SED (p = 0.025). Likewise, the fat oxidation during exercise was significantly much higher in EXE+SED (p = 0.016) compared to the difference between EXE and SED (p = 0.045). Then, the carbohydrate oxidation during exercise was significantly much lower in EXE+SED (p = 0.003) compared to the difference between EXE and SED (p = 0.028). [Conclusion] In conclusion, the anti-obesity functions of exercise training can be further enhanced by capsiate intake by increasing fat oxidation during exercise. Therefore, we suggest that capsiate could be a candidate supplement which can additively ameliorate obesity when combined with exercise.

Capsiate Intake with Exercise Training Additively Reduces Fat Deposition in Mice on a High-Fat Diet, but Not without Exercise Training

“…Subsequently, the adrenaline and noradrenaline levels in blood were enhanced by acute treatment with capsiate in humans [ 22 ] and rodents [ 21 ]; consequently, the released catecholamines promoted fat utilization and thermogenesis [ 31 , 32 ]. Indeed, CI before exercise decreased the respiratory exchange rate and increased fax oxidation during exercise [ 33 ]; moreover, according to the results of the current study, the β3AR protein expression of EXE+CAP was significantly upregulated in adipose tissue. This indicated that the ability of lipolysis was enhanced.…”

“…Note that the data of BW, FI, and abdominal fat, the result of Table 1 , were coused with the previously published paper [ 33 ].…”

Exogenous lactate augments exercise-induced improvement in memory but not in hippocampal neurogenesis

“…To ensure that the exercise intensity is equally maintained throughout the entire experiment (equalization of relative exercise intensity), we had gradually increased the treadmill speed and/or exercise duration over time (increasing absolute exercise intensity; Fig. 1 B), based on our previous studies 33 – 35 . The increase in absolute exercise intensity is required because chronic exercise enhances the exercise ability (occurrence of an exercise adaptation), and the enhanced exercise ability subsequently leads to decrease in the relative exercise intensity if the absolute exercise intensity is not increased.…”

Acute Administration of Exogenous Lactate Increases Carbohydrate Metabolism during Exercise in Mice

“…Prior to conducting the experiment, all groups were fasted for 8 h. The EXLA group was administered 3 g/kg of sodium lactate with an oral sonde and the EX group an equal amount of a saline solution [ 23 , 41 ]. The mice were orally administered the appropriate supplement 30 min before a moderate intensity (about 60–70% VO 2max ) exercise was conducted for 30 min (treadmill exercise at a speed of 18 m/min and slope of 6°) [ 42 , 43 ]. Immediately after exercise, the mice were anesthetized with 10 µL/g of 1.25% avertin and the tissues were collected.…”