Effect of minerals on the production of the delta endotoxin byBacillus thuringiensis subsp.israelensis

Sikdar, Deb Prasad; Majumdar, M. K.; Majumdar, S. K.

doi:10.1007/bf01049209

Cited by 23 publications

(12 citation statements)

References 5 publications

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“…This inferred that this component seems not necessary for the production of the crystal protein and spore in this case. It is tricky to say that CaCO 3 is an inhibitor for the spore production as the calcium is essential for cell growth and endotoxin production has been reported elsewhere [15]. Indeed, the calcium is required for the activation of amylase and exoprotease of B. thuringiensis during the vegetative growth and transition phases of metabolism [2].…”

Section: Resultsmentioning

confidence: 99%

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Liu

Tzeng

1998

Bioprocess Engineering

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The effects of cultivation medium compositions including tapioca, ®shmeal, CaCO 3 and (NH 4 ) 2 SO 4 for the growth of Bacillus thuringiensis YMB 96-1988 were accessed by using response surface methodology (RSM). The two-level (2 4±1 ) fractional factorial designs (FFD) which involve two concentrations of each nutrient, and the paths of steepest ascent were effective in searching for the major factors of the bacteria growth. This allows the ®tting of a ®rst order linear model to the data. In this study, supplementary CaCO 3 showed a negative effect on the spore production based on the ®rst order regression coef®cients derived from SAS programme. Subsequently, a 2 3 central composite design (CCD) was used for allocation of treatment combinations. Preliminary studies showed that tapioca and ®shmeal is believed to be the major factors for the growth of B. thuringiensis. Estimated optimum compositions for the production of spores by B. thuringiensis are as follows: tapioca, 5.01%; ®shmeal 5.86%; (NH 4 ) 2 SO 4 0.06% and resulted in a maximum spore count of 8.56´10 8 /ml was obtained. This value is close to the 8.35´10 8 /ml spore density as counted from actual experimental observations.

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Section: Resultsmentioning

confidence: 99%

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Liu

Tzeng

1998

Bioprocess Engineering

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“…According to Yang and Wang (1998), glucose is used in most large-scale Bt fermentation; however, high levels of glucose (> 40 g/liter) can cause growth inhibition. The amount of K, Mg, Ca, Fe, Mn and Cu found in the four tested media were enough to promote Bt growth (Sikdar et al 1991). Avignone Rossa et.…”

Section: Resultsmentioning

confidence: 99%

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Valicente¹,

Mourão²

2008

Neotrop. entomol.

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“…The Mg concentrations were above the value considered the minimum (0.3 g.dm -3 of MgSO 4 ) for optimum production of Bt israelensis toxins (SIKDAR et al, 1991 ) is necessary for Bt toxin production. This agrees with the data obtained.…”

Section: Resultsmentioning

confidence: 77%

“…The relationship between soil parameters and Bt presence showed that Cu 2+ possibly contributes for resistant spores formation by environmental stress or that it inhibits growth of antagonist microorganisms (HOSSAIN et al, 1997). Another element, Ca 2+ , is necessary for thermal stability of Bt spores, cellular development and δ-endotoxin production by this bacteria (DULMAGE & RHODES 1973;SIKDAR et al, 1991 . This is important because an adequate quantity of this element is essential to start Bt sporulation (DULMAGE & RHODES, 1973).…”

Section: Resultsmentioning

confidence: 99%

Relationship between chemical properties of the soil and the occurrence of Bacillus thuringiensis

2009

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The soil and other substrates such as mushroom compounds are the main sources of new Bacillus thuringiensis (Bt) isolates for Integrated Pest Management programs. This study describes the relationship between chemical properties of the soil (pH,OM INTRODUCTIONBacillus thuringiensis (Bt) is a widespread gram-positive spore-forming bacterium that produces toxic protein crystals named parasporal crystals. These inclusions are toxic to a wide variety of insects and many of them are commercially used against insect pests of Lepidoptera, Diptera and Coleoptera orders. Bt based biopesticides are the most successful ones and they represent approximately 80-90% of biological pest control agents worldwide (GLARE & O´CALLAGHAM, 2000;HABIB & ANDRADE, 1998 Relationship between chemical properties of the soil and the occurrence of Bacillus thuringiensisRelação entre as características químicas do solo e a ocorrência de Bacillus thuringiensis

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Effect of minerals on the production of the delta endotoxin byBacillus thuringiensis subsp.israelensis

Cited by 23 publications

References 5 publications

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Relationship between chemical properties of the soil and the occurrence of Bacillus thuringiensis

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