Effect of MiR-375 Regulates YAP1 on the Invasion, Apoptosis, and Epithelial-Mesenchymal Transition of Cervical Cancer HeLa Cells

Abstract

Yes-associated protein 1 (YAP1) is an important signaling pathway activator molecule. Studies have shown that it is involved in the occurrence of malignant tumors. This study identified a microRNA (miR/miRNA) targeting the 3′ untranslated region (3″ utr) of the YAP1 gene and evaluated its biological impact on human cervical cancer cells and related molecular mechanisms. qPCR and western blotting were used to detect the levels of miR-375 and YAP1 in HeLa cells. TargetScan software was used to identify the binding sites of YAP1 and miR-375. The MTT method was used to determine the viability of HeLa cells transfected with miR-375 mimic and YAP1 interference vector, the Transwell chamber experiment was used to detect the invasion of HeLa cells after transfection, the apoptosis of HeLa cells after transfection was detected by flow cytometry, and the western blotting was used to detect the epithelial mesenchymal transition (EMT) of HeLa cells after transfection. The expression of miR-375 in HeLa cells was significantly lower than that of normal control cervical cells, and the expression of YAP1 in HeLa cells was significantly higher than that of normal control cervical cells. TargetScan analysis showed that miR-375 was bound to the 3′ UTR of YAP1. qPCR and western blot analysis showed that transfection of miR-375 mimics inhibited YAP1 expression in HeLa cells. Transfection of miR-375 mimic and YAP1 interference vector inhibited HeLa cell invasion and EMT and promoted HeLa cell apoptosis. These findings indicate that miR-375 inhibits the malignant development of human cervical cancer cells by regulating the expression of YAP1.

Knockdown of microRNA-375 suppresses cell proliferation and promotes apoptosis in human breast cancer cells

“…Furthermore, several investigations were demonstrated that constitutive expression of miR-375 promotes oncogenicity in the breast (19)(20)(21)(22)(23) , prostate (24)(25)(26)(27) , and lung cancer (28) . In addition, few pieces of literature reported that miR-375 functions as a tumor suppressor (16,(29)(30)(31) . MiR-375 suppresses cervical cancer development by regulating epithelial-mesenchymal transition by targeting Yes-associated protein 1 (31) .…”

“…In addition, few pieces of literature reported that miR-375 functions as a tumor suppressor (16,(29)(30)(31) . MiR-375 suppresses cervical cancer development by regulating epithelial-mesenchymal transition by targeting Yes-associated protein 1 (31) . The exact role and function of the miR-375 in the breast cancer development remains uncertain.…”

Retracted: Effect of MiR‐375 Regulates YAP1 on the Invasion, Apoptosis, and Epithelial‐Mesenchymal Transition of Cervical Cancer HeLa Cells

“…This article has been retracted by Hindawi, as publisher, following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of systematic manipulation of the publication and peer-review process.…”