Effect of Mitogens on the Cell Cycle Progression and the Quantification of T-Lymphocyte Surface Markers in Acquired Immune Deficiency Syndrome

Hornicek, Francis J.; Malinin, George I.; Thornthwaite, Jerry T.; Whiteside, Mark E.; MacLeod, Carol L.; Malinin, Theodore I.

doi:10.1002/jlb.42.2.122

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…This is consistent with previous observations indicating that the elevation of the CD38*CD8* subset is a marker of poor prognosis [17,18]. Consequently, distinct CD8* cell subsets are associated with different stages of HIV disease: HIV-specific CD8* HLA-DR^ cells are elevated in asymptomatic HIV* patients [15,16] and with progression of disease HLA-DR is lost while CD38 is acquired. Our findings show that elevated levels of HLA-DR* cells are indicative of cells wilh an increased prolifcrative capacity, and elevated levels of CD38 ' cells may signify the presence of cells susceptible to apoptosis.…”

Section: Discussionsupporting

confidence: 92%

“…This notion is based on studies on lymphocytes isolated from patients with HIV infection/AIDS and cultured in vitro with mitogens before being assessed for their proliferative capacity [15]. In contrast, in the present study we directly assessed the cell cycle in circulating lymphocytes taken from HIV* patients, and studied without prior culture or exposure to mitogens.…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies in HIV infection have indicated that although both CD4* and CD8*T cell subsets expressed phenotypic markers of activation in vivo [2][3][4][5][6], these activated lymphocytes demonstrate a resistance to conventional activation stimuli in vitro [7][8][9]. This notion is based on studies on lymphocytes isolated from patients with HIV infection/AIDS and cultured in vitro with mitogens before being assessed for their proliferative capacity [15]. In contrast, in the present study we directly assessed the cell cycle in circulating lymphocytes taken from HIV* patients, and studied without prior culture or exposure to mitogens.…”

Section: Discussionmentioning

confidence: 99%

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Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Mahalingam

Pozniak

McManus

et al. 1995

Clinical and Experimental Immunology

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SUMMARY Infection with HIV results in increased circulating levels of T lymphocytes expressing phenotypic markers of immune activation. In the present study, using three‐colour immunofluorescence, we examined the cell cycle status of these activated cells. Activated (HLA‐DR+ CD25+ and CD38+) CD4+ and CD8+ T lymphocytes in peripheral blood were analysed for DNA content in 15 HIV + patients and 10 healthy age‐ and sex‐matched control subjects. As expected, all HIV+ patients had elevated percentage levels of activated CD4+ HLA‐DR+, CD4+ CD25+ CD8+ HLA‐DR+ CD8+ CD25+ and CD8+ CD38+ T lymphocytes compared with control subjects (P<0.001 for all). Percentage levels of CD4+ HLA‐DR+ and CD8+ HLA‐DR+ T lymphocytes in the ‘proliferative’(S‐G2M) phase of the cell cycle were also higher in the HIV+” patients compared with controls (P<0.001 for both). The percentage levels of proliferative CD4+ CD25+ CD8+ CD25+ and CD8+ CD38+ lymphocytes were, however, similar in HIV+ patients and controls, indicating that the proliferative fraction of cells in vivo was confined to the HLA‐DR+ subset and absent from the CD25+ and CD38+ populations. Four HIV+ patients had grossly elevated levels of CD8+ lymphocytes which were CD38+(>95%) and confined to the pre‐G0‐G1 phase of the cell cycle, suggesting these may be cells committed to apoptosis. These observations indicate an increase in the proliferative capacity of HLA‐DR+ T lymphocytes in HIV infection in vivo. The reduced DNA content in other populations (e.g. CD38+ CD8+ lymphocytes) of some patients with advanced HIV disease suggests that these cells are apoptotic. Thus our results define both proliferative and apoptotic processes as a spectrum of activation‐related events in HIV infection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Mahalingam

Pozniak

McManus

et al. 1995

Clinical and Experimental Immunology

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“…Within 6 hr of head injury, venous blood was drawn and PBLs were isolated essentially as described by Hornicek et al (1987). Venous blood was collected and mononuclear cells isolated by density gradient.…”

Section: Isolation Of Peripheral Blood Lymphocytes and Mitogen Stimulmentioning

confidence: 99%

Impairment of Helper T-Cell Function Following Severe Head Injury

Quattrocchi

Issel²,

Miller³

et al. 1992

Journal of Neurotrauma

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Major infections, such as sepsis and pneumonia, occur in 50-75% of patients following isolated severe head injury. Previous studies have demonstrated that this high incidence of infection following severe head injury may be related to a decrease in helper T-cell activation and function. The present study was designed to investigate the effect of severe head injury on specific subgroups of helper T cells known to enhance or suppress cellular immune function. Specifically, peripheral blood lymphocytes (PBLs) from 10 head-injured patients and 10 matched controls were evaluated following in vitro stimulation with the T-cell mitogen, phytohemagglutinin (PHA). Subsets of helper T cells evaluated included activated helper (CD4+/CD25+) T cells; helper/inducer (CD4+/CDw29+) T cells, which enhance cellular immune activity; and suppressor/inducer (CD4+/CD45R+) T-cells, which induce suppressor (CD8+) T-cells. In addition, the effect of intraventricular fluid (IVF) on PHA-stimulated in vitro CD4 and CD25 expression was investigated to determine whether severe head injury results in the production of mediators within the central nervous system capable of affecting T-cell activation. The results of this study indicate that isolated severe head injury selectively reduces the ability of PHA-stimulated PBLs to express the helper/inducer (CD4+/CDw29+) T-cell (p = 0.023) and activated helper (CD4+/CD25+) T-cell (P = 0.041) phenotypes. There was no significant change in PHA-stimulated CD4 or CD25 expression following incubation of PBLs with intraventricular fluid (IVF) from head-injured patients. The relationship between these changes in specific helper T-cell subpopulations and the infectious complications of severe head injury are discussed.

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Humoral and cellular immunity following severe head injury: review and current investigations^‡

Quattrocchi¹,

Frank²,

Issel³

et al. 1991

Neurological Research

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Effect of Mitogens on the Cell Cycle Progression and the Quantification of T-Lymphocyte Surface Markers in Acquired Immune Deficiency Syndrome

Cited by 5 publications

References 15 publications

Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Impairment of Helper T-Cell Function Following Severe Head Injury

Humoral and cellular immunity following severe head injury: review and current investigations^‡

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Effect of Mitogens on the Cell Cycle Progression and the Quantification of T-Lymphocyte Surface Markers in Acquired Immune Deficiency Syndrome

Cited by 5 publications

References 15 publications

Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Cell cycling in HIV infection: analysis ofin vivoactivated lymphocytes

Impairment of Helper T-Cell Function Following Severe Head Injury

Humoral and cellular immunity following severe head injury: review and current investigations‡

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Product

Resources

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Humoral and cellular immunity following severe head injury: review and current investigations^‡