Effect of mobile phone electromagnetic waves on rat testis and the possible ameliorating role of Naringenin : A histological study

Abstract

Introduction: With increasing the use of cell phones, the interest of examining the hazards that could affect health of people, which may or may not lead to cancer, infertility, or birth defects, was elevated. Recently, a natural flavonoid compound, Naringenin (NG) had received considerable attention as an antioxidant. Aim of the work: Evaluating the effects of mobile phone use on the testes of adult rats, the progress of spontaneous recovery and the protective role of NG supplementation against these effects. Materials and Methods: Twenty-four adult male albino rats were equally divided into four groups; control (I), (II) mobile, (III) mobile-NG and (IV) recovery. Mobile group exposed to 900 MHz continuous RF-EMW emitted by talk mode for one hour daily for 8 weeks. Mobile-NG group exposed to RF-EMW like group III for 8 weeks concomitant with NG treatment 50 mg/kg /day orally. While recovery group exposed to the same RF-EMW for 8 weeks then kept unexposed for another 8 weeks for recovery. Serum levels of testosterone hormone, epididymal sperm counts and testicular malondialdehyde (MDA) level were measured. Testicular sections were stained with H&E and immunohistochemical stains for PCNA, p53, CX43 and AR, that were subjected to morphometric and statistically analysis. Results: Deterioration of histological architecture of testes and biochemical and morphometric parameters were recorded in mobile group. However, preservation of the testicular histological structure and restoration of the normal biochemical and morphometric parameters were obvious in group IV. On the other hand, the recovery group showed incomplete improvement where some testicular affection was noted. Conclusion: Mobile phones might have injurious effects on testes that partially recovered after stoppage of exposure to EMW. However, NG co-administration was protective.

Cytoprotective potency of naringin against di-n-butylphthalate (DBP)-induced oxidative testicular damage in male rats

“…Meanwhile, administration of NG at dose levels of 40, 80, 160 mg/kg for 30 days improved the testicular function and structure in BPA-intoxicated rats, proved by the increased the epididymal sperm count, testicular enzymes, serum hormonal levels, antioxidant enzymes activities and improved the testicular morphology with increasing the diameter, and epithelial height of seminiferous tubules (Alboghobeish et al 2019 ). Noteworthy, previous literatures proved also the protective effect of naringenin, the main metabolite of NG, against the impairment of the function and histological architecture of testes induced by hydrogen peroxide toxicity (Sahin et al 2017 ) or by the mobile phone electromagnetic waves (Farag and Yousry 2018 ).…”

“…The recoded improvement in sperm motility, count and viability might be due to obvious protective effect of NG on the Leydig cells, which accompanied by releasing more testosterone, which is essential for normal spermatogenesis (Farag and Yousry 2018 ) and also could be due to the suppression of ROS-induced disturbances in sperm and germ cells (Alboghobeish et al 2019 ). It is well-known that antioxidants protect DNA and other important molecules from oxidative damage, and can improve sperm quality, and consequently, increase male fertility (Bansal and Bilaspuri 2011 ).…”

The effect of Zinc Nanoparticles on Adult rat Prostate gland and the Possible Protective role of Rutin: histological and biochemical study

“…Secondary antibodies (Goat antibodies, catalog number ab205718, Abcam, Cambridge, UK) was applied to the slide, diluted in TBS with 1% BSA, and incubated for 1 h at room temperature. Negative control sections were performed with the same procedure, but the primary antibody was non-immune rabbit serum [21] .…”

An Experimental Investigation of the Impact of Electromagnetic Radiations Emitted from Mobile Phone on General Health, pH, Flow Rate and Electrolytes Concentrations of Saliva in Female Adults

“…For example Kaur and Khera [35] studied the impact of cell phone radiations on pituitary gland and biochemical parameters in albino rat. Special focus was also carried out on the effect of mobile phone radiation on spermatogenesis [36], testicular function [37], brain structure and functions [38], thyroid glands [39], heart tumors [40], anxiety level [41] and on blood factors [42]. In addition, Wyde et al [43] studied the effects of cell phone radiation on body temperature in rodents.…”