Effect of Modified Ketogenic Diets on Rats with induced Obesity

Ibrahem, Eman; Mostafa, Usama; Ali, Mostafa Z.; Mohamed, Marwa M.

doi:10.21608/ejnh.2021.239552

Cited by 2 publications

(2 citation statements)

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“…The basal diet of the rats was prepared following the laboratory animal diet guidelines as previously described [ 20 ]. The ketogenic diet was prepared according to [ 21 ]. The chitosan diet was prepared as a basal diet + 5% low-MW (91 kDa) chitosan according to [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

The effects of ketogenic and chitosan-based diets on submandibular salivary gland in rat model: a comparative histological study

Aboulfotoh

2024

BMC Oral Health

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Objective This study was carried out in the submandibular salivary glands (SSGs) of rats to demonstrate the effect of a ketogenic diet (KD) in comparison with dietary chitosan supplementation. Method Eighteen albino rats were randomly divided into three equal groups of six animals each. Rats in Group I were fed a balanced diet and considered controls. Meanwhile, those of Groups II and III were fed a KD, a balanced diet with high molecular weight chitosan, respectively. After 45 days, rats were euthanized, and the SSGs were dissected carefully for staining with hematoxylin and eosin (H&E), alpha-smooth muscle actin (α-SMA) immunohistochemical staining, and Congo red special stain. Quantitative data from α-SMA staining and Congo red staining were statistically analyzed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. Results Regarding Congo red and α-SMA staining, one-way ANOVA revealed a significant difference between the three groups. For α-SMA staining and Congo red staining, Group II had the highest mean values of 91.41 ± 3.30 and 68.10 ± 5.04, respectively, while Group I had the lowest values of 56.13 ± 3.96 and 16.87 ± 2.19, respectively. Group III had mean values of 60.70 ± 3.55 for α-SMA and 19.50 ± 1.78 for Congo red. Tukey’s multiple comparisons post hoc test revealed significant differences between groups I & II and between groups II & III (P < 0.05). Meanwhile, there was a nonsignificant difference between groups I and III (P > 0.05). Conclusion A KD has a deleterious effect on rats’ SSG whatever the test we used, and dietary chitosan supplementation ameliorates these damaging effects.

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Section: Methodsmentioning

confidence: 99%

The effects of ketogenic and chitosan-based diets on submandibular salivary gland in rat model: a comparative histological study

Aboulfotoh

2024

BMC Oral Health

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show abstract

“…The basal diet of the rats was prepared following the laboratory animal diet guidelines as previously described [19]. The ketogenic diet was prepared according to [20]. The chitosan diet was prepared as a basal diet + 5% low-MW (91 kDa) chitosan according to [21].…”

Section: Diet Preparationmentioning

confidence: 99%

Comparative Effect of Ketogenic and Chitosan-Based Diets on Submandibular Salivary Gland Morphology in Rats: A Histological and Immuno-Histochemical Analysis

Aboulfotoh

2023

Preprint

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Objective: This study was carried out in the submandibular salivary glands (SSGs) of rats to demonstrate the effect of a ketogenic diet (KD) in comparison with dietary chitosan supplementation. Method: Eighteen albino rats were randomly divided into three equal groups. Rats in Group I were fed a balanced diet and considered controls. Meanwhile, those of Groups II and III were fed a KD, a balanced diet with high molecular weight chitosan, respectively. After 45 days, rats were euthanized, and the SSGs were dissected carefully for staining with hematoxylin and eosin (H&E), alpha-smooth muscle actin (α-SMA) immunohistochemical staining, and Congo red special stain. Quantitative data from α-SMA staining and Congo red staining were statistically analyzed using one-way ANOVA followed by Tukey's multiple comparisons post hoc test. Results: Regarding Congo red and α-SMA staining, one-way ANOVA revealed a significant difference between the three groups. For α-SMA staining and Congo red staining, Group II had the highest mean values of 91.41 ± 3.30 and 68.10 ± 5.04, respectively, while Group I had the lowest values of 56.13 ± 3.96 and 16.87 ± 2.19, respectively. Group III had mean values of 60.70 ± 3.55 for α-SMA and 19.50 ± 1.78 for Congo red. Tukey's multiple comparisons post hoc test revealed significant differences between groups I & II and between groups II & III (P ˂ 0.05). Meanwhile, there was a nonsignificant difference between groups I and III (P ˃ 0.05). Conclusion: A KD has a deleterious effect on rats' SSG, and dietary chitosan supplementation ameliorates these damaging effects.

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Effect of Modified Ketogenic Diets on Rats with induced Obesity

Cited by 2 publications

References 0 publications

The effects of ketogenic and chitosan-based diets on submandibular salivary gland in rat model: a comparative histological study

The effects of ketogenic and chitosan-based diets on submandibular salivary gland in rat model: a comparative histological study

Comparative Effect of Ketogenic and Chitosan-Based Diets on Submandibular Salivary Gland Morphology in Rats: A Histological and Immuno-Histochemical Analysis

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