Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

Ñuñez-Gaona, Óscar; Saucedo‐Castañeda, Gerardo; Alatorre-Rosas, Raquel; Loera, Octavio

doi:10.1590/s1516-89132010000400004

Cited by 44 publications

(30 citation statements)

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“…Similar response of moisture content on inulinase production by Pichia guilliermondii in SSF has also been reported (18). Nuñez-Gaona et al (19) also reported that only a certain level of moisture is enough to accelerate nutrient absorption, normal metabolic function and fungal conidial production. Requirement of moisture content can vary from species to species.…”

Section: Effect Of Independent Variables and Their Interactions On Insupporting

confidence: 73%

“…Requirement of moisture content can vary from species to species. Generally, minimum of 20 % of moisture level is essential for fungal growth, while very high moisture content may retard conidial induction (19). The increase in pH up to 7.0 has also shown a significant influence on inulinase production.…”

Section: Effect Of Independent Variables and Their Interactions On Inmentioning

confidence: 99%

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Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4

Singh¹,

Chauhan²,

Singh³

et al. 2017

Food Technol. Biotechnol.

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SUMMARYInulinases are an important class of industrial enzymes which are used for the production of high-fructose syrup and fructooligosaccharides. Inulin, a polyfructan, is generally employed for the production of inulinase, which is a very expensive substrate. A number of agroindustrial residues have been used for cost-effective production of inulinases. In the present study, carrot pomace was selected as a substrate for the production of inulinase by Penicillium oxalicum BGPUP-4 in solid-state fermentation. Carrot pomace is one of the good substrates for bioprocesses, because it is rich in soluble and insoluble carbohydrates. A central composite rotatable design (CCRD) used in response surface methodology was employed for the optimal production of inulinase from carrot pomace. Using CCRD, 15 runs were practiced to optimize the range of three independent variables: moisture content (70-90 %), incubation time (4-6 days) and pH (5.0-7.0) for inulinase production. Carrot pomace supplemented with 0.5 % inulin as an inducer, 0.2 % NH 4 H 2 PO 4 , 0.2 % NaNO 3 , 0.2 % KH 2 PO 4 , 0.05 % MgSO 4 ·7H 2 O and 0.001 % FeSO 4 ·7H 2 O was used for the production of inulinase in solid-state fermentation at 30 °C. Inulinase production (322.10 IU per g of dry substrate) was obtained under the optimized conditions, i.e. moisture content of 90 %, incubation time 4 days and pH=7.0. The corresponding inulinase/invertase (I/S) ratio (3.38) was also high, which indicates the inulolytic nature of the enzyme. Multiple correlation coefficients R for inulinase production and I/S ratio were 0.9995 and 0.9947, respectively. The R value very close to one indicates an excellent correlation between experimental and predicted results.

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Section: Effect Of Independent Variables and Their Interactions On Insupporting

confidence: 73%

Section: Effect Of Independent Variables and Their Interactions On Inmentioning

confidence: 99%

Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4

Singh¹,

Chauhan²,

Singh³

et al. 2017

Food Technol. Biotechnol.

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“…Commonly an inoculum concentration of 1 × 10 7 spores g -1 is recommended (Arzumanov et al 2005;Nuñez-Gaona et al 2010). In the present study, the statistical analysis suggested that it was possible to choose an inoculum level between 10 6 and 10 7 spores g -1 that resulted in the same spore production.…”

Section: Optimization For Spore Production Via Bbmmentioning

confidence: 58%

“…A marked decrease in spore production was pointed out when the inoculum level was 1 × 10 9 spores g -1 . Inoculum levels have also been evaluated for other fungal biocontrol agents such as Beauveria bassiana by Nuñez-Gaona et al (2010), who tested concentrations of 1 × 10 6 , 7 × 10 6 , and 5 × 10 7 spores g -1 through SSF of wheat bran. The three levels of inoculum evaluated resulted in high spore production (1.08 × 10 10 to 1.53 × 10 10 spores g -1 ).…”

Section: Optimization For Spore Production Via Bbmmentioning

confidence: 99%

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Utilization of Lignocellulose-based Orange Peel Waste for Induced Sporulation of Trichoderma asperellum via Box-Behnken Matrix Design

Quiroz¹,

Carrillo-Nieves²,

Aguilar‐Zárate³

et al. 2018

BioResources

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The feasibility of using orange peel residues as a substrate for induced sporulation of Trichoderma asperellum was evaluated. The Box-Behnken matrix (BBM) design was used to screen the effects of several parameters, including the effect of pH, inoculum, and moisture under solid-state fermentation culture conditions. The study was performed in two experimental steps (screening and optimization). Moisture content and pH were determined to be the most influential parameters on spore production during the screening stage. A Box-Behnken design was used to optimize and to define the interaction of the selected parameters. The moisture content was determined as the most significant parameter affecting spore production. An inoculum of 1 × 10 6 spores g -1 , pH 6.07, and moisture content of 69.0% was the combination of conditions observed to achieve the maximum production of 2.04 × 10 9 spores g -1 . The experimental value of 2.16 × 10 9 spores g -1 (from the experimental model) showed a good fit to the regressed model, with a standard error of 5%. Based on this work, a high yield of spores was obtained at 144 h of cultivation time, indicating that it is a feasible approach to use orange peel as a substrate for biomass and spore production.

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Production of Conidia by the Fungus Metarhizium anisopliae Using Solid-State Fermentation

Loera

Porcayo-Loza

Montesinos-Matías

et al. 2016

Methods in Molecular Biology

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This chapter describes the production of conidia by Metarhizium anisopliae using solid-state fermentation. Before production of conidia, procedures for strains conservation, reactivation, and propagation are essential in order to provide genetic stability of the strains. The strain is conserved in freeze-dried vials and then reactivated through insect inoculation. Rice is used as a substrate for the conidia production in two different bioreactors: plastic bags and tubular bioreactor. The CO2 production in the tubular bioreactors is measured with a respirometer; this system allows calculating indirect growth parameters as lag time (tlag) (25-35 h), maximum rate of CO2 production (rCO2 max) (0.5-0.7 mg/gdm h), specific rate of CO2 production (μ) (0.10-0.15 1/h), and final CO2 production (CO2) (100-120 mg/gdm). Conidial yield per gram of dry substrate (gdm) should be above 1 × 10(9) conidia/gdm after 10 days of incubation. Germination and viability of conidia obtained after 10 days of incubation should be above 80 % and 75 %, respectively. Bioassays using of Tenebrio molitor as a host insect should yield a final mortality above 80 %.

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Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

Abstract: The aim of the present work was to study the effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran (WB)

Cited by 44 publications

References 20 publications

Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4

Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4

Utilization of Lignocellulose-based Orange Peel Waste for Induced Sporulation of Trichoderma asperellum via Box-Behnken Matrix Design

Production of Conidia by the Fungus Metarhizium anisopliae Using Solid-State Fermentation

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