Effect of moisture on sealing ability of root canal filling with different types of sealer through the glucose penetration model

Abstract

Objectives: To compared the effect of different levels of moisture of root canal on the sealing ability after filling with four different types of sealer. Materials and Methods: Single-rooted teeth (n = 90) instrumented to and apical size of 0.06 / 45 were randomly assigned to 12 experimental groups (n = 7 per group), positive/negative control groups (n = 3 per group). The teeth of the experimental groups (a. DRY; b. PAPER POINT DRY; c. WET) were obturated with sealer (Group 1-3: Sealapex; Group 4-6: AH plus; Group 7-9: Tubuli-seal; Group 10-12: EndoRez) and warm vertical compaction method. After 7 days in 37℃, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometer at 1, 3, 7, 14, 21, and 30 days. Data were recorded ad mmol/L and statistically analysed with the two-way ANOVA and Duncan test (p = 0.05). Results: Throughout the experimental period Tubuli-seal/WET (Group 9) showed the highest mean cumulative glucose penetration (178.75 mmol/L), whereas AH plus/DRY (Group 4) had the least (20.78 mmol/L). Conclusions:The results of this study demonstrated that the moisture condition of root canals at the time of obturation and the type of sealer that was used had a significant effect on leakage and sealing ability. Thus drying procedure according to sealer types is a critical step and should not be missed in endodontic treatment.

Evaluation of softening ability of Xylene & Endosolv-R on three different epoxy resin based sealers within 1 to 2 minutes - anin vitrostudy

“… 15 Further microleakage has been found to be least in the case of resin based sealers. 16 Cho et al have also mentioned in their study that the bond strength of final restoration was the least affected in case of resin based sealers than the zinc oxide euginol root canal sealer. 17 Kim et al have stated that resin based sealers (AH 26, EZ fill and AD Seal) are more biocompatible and have advantage in terms of radiopacity.…”

“…Mamootil et al have stated that resin-based sealers have deeper and more consistent penetration into dentinal tubules than other sealers both in vitro and in vivo 15. Further microleakage has been found to be least in the case of resin based sealers 16. Cho et al have also mentioned in their study that the bond strength of final restoration was the least affected in case of resin based sealers than the zinc oxide euginol root canal sealer 17.…”

Comparing the apical micro-leakage caused by various endodontic sealers in the presence and absence of moisture Single Author

“…The results of tests conducted in both damp and dry environments showed that the AD Seal sealer had the lowest micro-leakage and that the kind of moisture (5.25% hypochlorite or blood) had no discernible impact on the rate of sealer micro-leakage [19]. Jinah et al's [19] research showed that the type of sealer employed and the moisture level of the root canals at the time of obturation had a big impact on leakage and sealing ability. Therefore, drying procedures according to sealer types are a crucial stage in endodontic treatment and shouldn't be skipped [28,29].…”

Evaluation of microleakage of three different endodontic sealers in the presence and absence of moisture

“…On the other hand, some other researchers such as Jang et al (31) and Goldman et al (5) suggested totally dried root canals before the obturation process because they believed it may increase the adherence of the filling material to the dentin walls of the canal.…”