Effect of Molasses Addition at Ensiling on Ruminal &amp;lt;i&amp;gt;In Situ&amp;lt;/i&amp;gt; Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

Ghizzi, Lucas G.; Pupo, Matheus R.; Heinzen, Celso; Valle, Tiago A. Del; Rennó, Francisco Palma; Ferraretto, L.F.

doi:10.4236/as.2022.132018

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…This could translate into low enteric methane because methanogens (archaea bacteria) use H 2 and CO 2 gases to produce enteric methane [63]. In addition, NDF degradability is regarded as a good indicator of dry matter disappearance [64]. Therefore, this study showed that increasing the inclusion level of the accession 11,604 to 30% improves the efficient utilization of low-quality grass hay by the rumen microbes.…”

Section: Discussionmentioning

confidence: 82%

Effects of Stylosanthes scabra Forage Supplementation on in Vitro Gas Production and Fiber Degradation of Eragrostis Grass Hay

Mpanza¹,

Hassen²

2023

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Natural pastures constitute a major component of ruminant livestock feed, and are the most cost-effective feed resource available for smallholder subsistence farmers. However, this feed resource does not meet animal nutritional requirement due to deficiency in nitrogen, energy and minerals. In addition, at maturity lignification is the major concern since it reduces digestibility and contributes to methane emission. Thus, the objective of this study was to evaluate the effect of supplementing low-quality Eragrostis grass hay with five (9281, 11,252, 11,255, 11,595 and 11,604) selected Stylosanthes scabra accessions on in vitro ruminal fermentation and neutral detergent fiber degradation. Therefore, in vitro study was conducted on grass hay, accessions and the mixture of grass hay with each accession included at two (15%, 30%) levels. The substrates (grass hay, accessions and the mixtures) were incubated in separate serum bottles for 72 h. Neutral detergent fiber (NDF) of the accessions ranged from 300 to 350 g/kg DM with crude protein (CP) value ranging from 177.5 to 184.1 g/kg DM. Eragrostis grass hay had NDF value of 813 g/kg DM, with CP value of 34.3 g/kg DM. Grass hay fermented slowly, it took 30 h for grass hay to produce gas volume above 50 mL, while Stylosanthes scabra accessions took 12 h. Supplementing grass hay with accessions significantly improved fermentation. However, it was observed that 15% inclusion took 30 h to produce gas volume above 50 mL, whereas at 30% inclusions it took 24 h for accession 9281,11,595 and 11,604. Accession 11,604 improve grass fermentation by almost three times the value of grass hay in 2 h. Grass hay supplemented with accession 11,604 at 30% had a positive associative effect and significantly improved NDF degradability. In conclusion, accession 11,604 may be fed strategically as forage supplement to low-quality forage for ruminants.

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Section: Discussionmentioning

confidence: 82%

Effects of Stylosanthes scabra Forage Supplementation on in Vitro Gas Production and Fiber Degradation of Eragrostis Grass Hay

Mpanza¹,

Hassen²

2023

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“…Whole-plant soybeans, as legumes, offer high yields, ease of harvest, and high protein and vitamin content with good palatability and digestibility ( Gandra et al, 2022 ). However, their lower water-soluble carbohydrate (WSC) content and higher buffering energy lead to higher pH in whole-plant soybean silage, limiting LAB growth and challenging good silage production ( Ghizzi et al, 2022 ). In contrast, whole-plant corn, a graminaceous plant, serves as a primary roughage source in many countries.…”

Section: Introductionmentioning

confidence: 99%

Effects of lactic acid bacteria inoculants on the nutrient composition, fermentation quality, and microbial diversity of whole-plant soybean-corn mixed silage

Xu,

Ma,

et al. 2024

Front. Microbiol.

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The mixture of whole-plant soybean and whole-plant corn silage (WPSCS) is nutrient balanced and is also a promising roughage for ruminants. However, few studies have investigated the changes in bacterial community succession in WPSCS inoculated with homofermentative and heterofermentative lactic acid bacteria (LAB) and whether WPSCS inoculated with LAB can improve fermentation quality by reducing nutrient losses. This study investigated the effect of Lactobacillus plantarum (L. plantarum) or Lactobacillus buchneri (L. buchneri) on the fermentation quality, aerobic stability, and bacterial community of WPSCS. A 40:60 ratio of whole-plant soybean corn was inoculated without (CK) or with L. plantarum (LP), L. buchneri (LB), and a mixture of LP and LB (LPB), and fermented for 14, 28, and 56 days, followed by 7 days of aerobic exposure. The 56-day silage results indicated that the dry matter content of the LP and LB groups reached 37.36 and 36.67%, respectively, which was much greater than that of the CK group (36.05%). The pH values of the LP, LB, and LPB groups were significantly lower than those of the CK group (p < 0.05). The ammoniacal nitrogen content of LB was significantly lower than that of the other three groups (p < 0.05), and the ammoniacal nitrogen content of LP and LPB was significantly lower than that of CK (p < 0.05). The acetic acid content and aerobic stability of the LB group were significantly greater than those of the CK, LP, and LPB groups (p < 0.05). High-throughput sequencing revealed a dominant bacteria shift from Proteobacteria in fresh forage to Firmicutes in silage at the phylum level. Lactobacillus remained the dominant genus in all silage. Linear discriminant analysis effect size (LEFSe) analysis identified Lactobacillus as relatively abundant in LP-treated silage and Weissella in LB-treated groups. The results of KEGG pathway analysis of the 16S rRNA gene of the silage microbial flora showed that the abundance of genes related to amino acid metabolism in the LP, LB, and LPB groups was lower than that in the CK group (p < 0.05). In conclusion, LAB application can improve the fermentation quality and nutritional value of WPSCS by regulating the succession of microbial communities and metabolic pathways during ensiling. Concurrently, the LB inoculant showed the potential to improve the aerobic stability of WPSCS.

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Effect of Molasses Addition at Ensiling on Ruminal <i>In Situ</i> Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

Cited by 2 publications

References 22 publications

Effects of <i>Stylosanthes scabra</i> Forage Supplementation on <i>in Vitro</i> Gas Production and Fiber Degradation of <i>Eragrostis</i> Grass Hay

Effects of <i>Stylosanthes scabra</i> Forage Supplementation on <i>in Vitro</i> Gas Production and Fiber Degradation of <i>Eragrostis</i> Grass Hay

Effects of lactic acid bacteria inoculants on the nutrient composition, fermentation quality, and microbial diversity of whole-plant soybean-corn mixed silage

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Effect of Molasses Addition at Ensiling on Ruminal &lt;i&gt;In Situ&lt;/i&gt; Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

Cited by 2 publications

References 22 publications

Effects of &lt;i&gt;Stylosanthes scabra&lt;/i&gt; Forage Supplementation on &lt;i&gt;in Vitro&lt;/i&gt; Gas Production and Fiber Degradation of &lt;i&gt;Eragrostis&lt;/i&gt; Grass Hay

Effects of &lt;i&gt;Stylosanthes scabra&lt;/i&gt; Forage Supplementation on &lt;i&gt;in Vitro&lt;/i&gt; Gas Production and Fiber Degradation of &lt;i&gt;Eragrostis&lt;/i&gt; Grass Hay

Effects of lactic acid bacteria inoculants on the nutrient composition, fermentation quality, and microbial diversity of whole-plant soybean-corn mixed silage

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Effect of Molasses Addition at Ensiling on Ruminal <i>In Situ</i> Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

Effects of <i>Stylosanthes scabra</i> Forage Supplementation on <i>in Vitro</i> Gas Production and Fiber Degradation of <i>Eragrostis</i> Grass Hay

Effects of <i>Stylosanthes scabra</i> Forage Supplementation on <i>in Vitro</i> Gas Production and Fiber Degradation of <i>Eragrostis</i> Grass Hay