Effect of molecular weight and tissue layer on solute partitioning in the knee meniscus

Morejon, Andy; Schwartz, Gabi; Best, Thomas M.; Travascio, Francesco; Jackson, Alicia R.

doi:10.1016/j.ocarto.2023.100360

Cited by 4 publications

(3 citation statements)

References 59 publications

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“…Previous studies have recorded diffusivity of probes with a wide range of hydrodynamic radii in human and animal menisci [ 6 , 10 , 12 ]. In addition, molecular solubility has been studied across meniscal regions [ 26 ]. However, to the authors’ best knowledge, this is the first study reporting molecular and macromolecular diffusivities across different meniscal regions.…”

Section: Discussionmentioning

confidence: 99%

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Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue

Schwartz,

Best,

Chen

et al. 2024

PLoS ONE

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Diffusion within extracellular matrix is essential to deliver nutrients and larger metabolites to the avascular region of the meniscus. It is well known that both structure and composition of the meniscus vary across its regions; therefore, it is crucial to fully understand how the heterogenous meniscal architecture affects its diffusive properties. The objective of this study was to investigate the effect of meniscal region (core tissue, femoral, and tibial surface layers) and molecular weight on the diffusivity of several molecules in porcine meniscus. Tissue samples were harvested from the central area of porcine lateral menisci. Diffusivity of fluorescein (MW 332 Da) and three fluorescence-labeled dextrans (MW 3k, 40k, and 150k Da) was measured via fluorescence recovery after photobleaching. Diffusivity was affected by molecular size, decreasing as the Stokes’ radius of the solute increased. There was no significant effect of meniscal region on diffusivity for fluorescein, 3k and 40k dextrans (p>0.05). However, region did significantly affect the diffusivity of 150k Dextran, with that in the tibial surface layer being larger than in the core region (p = 0.001). Our findings contribute novel knowledge concerning the transport properties of the meniscus fibrocartilage. This data can be used to advance the understanding of tissue pathophysiology and explore effective approaches for tissue restoration.

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Section: Discussionmentioning

confidence: 99%

“…Differences in composition and structure have been shown to correlate with tissue mechanical properties [20]. In addition, previous studies have shown that diffusivity in cartilaginous tissues is inversely related to the molecular weight of the solute [6,12,14,[26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue

Schwartz,

Best,

Chen

et al. 2024

PLoS ONE

Self Cite

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“…This entails that the permeability tensor is dependent not solely on pressure, but is also contingent on a temporary basis by its spatial location within the meniscus due to local variation of applied pressure gradients. Recent work on the meniscus highlighted the link between biomechanical and physical properties such as fibre orientation, tissue layer, and water content [14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

On the Characteristics of Natural Hydraulic Dampers: An Image-Based Approach to Study the Fluid Flow Behaviour Inside the Human Meniscal Tissue

Waghorne,

Bonomo,

Rabbani

et al. 2023

Preprint

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Human mesenchymal stem/stromal cell‐derived extracellular vesicle transport in meniscus fibrocartilage

Schwartz,

Rana,

Jackson

et al. 2024

Journal Orthopaedic Research

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Extracellular vesicles (EVs) derived from endometrial‐derived mesenchymal stem/stromal cells (eMSC) play a crucial role in tissue repair due to their immunomodulatory and reparative properties. Given these properties, eMSC EVs may offer potential benefits for meniscal repair. The meniscus, being partly vascularized, relies on diffusivity for solute trafficking. This study focuses on EVs transport properties characterization within fibrocartilage that remains unknown. Specifically, EVs were isolated from Crude and CD146+ eMSC populations. Green fluorescence‐labeled EVs transport properties were investigated in three structurally distinct layers (core, femoral, and tibial surfaces) of porcine meniscus. Diffusivity was measured via custom fluorescence recovery after photobleaching (FRAP) technique. Light spectrometry was used to determine EVs solubility. Both Crude and CD146+ eMSC EVs exhibited high purity (>90% CD63CD9 marker expression) and an average diffusivity of 10.924 (±4.065) µm²/s. Importantly, no significant difference was observed between Crude and CD146+ eMSC EV diffusivity on the meniscal layer (p > 0.05). The mean partitioning coefficient was 0.2118 (±0.1321), with Crude EVs demonstrating significantly higher solubility than CD146+ EVs (p < 0.05). In conclusion, this study underscores the potential of both Crude and CD146+ eMSC EVs to traverse all layers of the meniscus, supporting their capacity to enhance delivery of orthobiologics for cartilaginous tissue healing.

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Effect of molecular weight and tissue layer on solute partitioning in the knee meniscus

Cited by 4 publications

References 59 publications

Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue

Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue

On the Characteristics of Natural Hydraulic Dampers: An Image-Based Approach to Study the Fluid Flow Behaviour Inside the Human Meniscal Tissue

Human mesenchymal stem/stromal cell‐derived extracellular vesicle transport in meniscus fibrocartilage

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