1998
DOI: 10.1046/j.1432-1327.1998.2550755.x
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Effect of molybdate and tungstate on the biosynthesis of CO dehydrogenase and the molybdopterin cytosine‐dinucleotide‐type of molybdenum cofactor in Hydrogenophaga pseudoflava

Abstract: The molybdenum-containing iron-sulfur flavoprotein CO dehydrogenase is expressed in a catalytically fully competent form during heterotrophic growth of the aerobic bacterium Hydrogenophaga pseudoflava with pyruvate plus CO. We have adopted these conditions for studying the effect of molybdate (Mo) and tungstate (W) on the biosynthesis of CO dehydrogenase and its molybdopterin (MPT) cytosine-dinucleotide-(MCD)-type molybdenum cofactor. W was taken up by the Mo transport system and, therefore, interfered with Mo… Show more

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Cited by 38 publications
(42 citation statements)
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“…It has been reported previously that cell extracts from H. pseudoflava grown in the absence of molybdate did not produce MCD (28). In addition, CO dehydrogenase expressed under these conditions was devoid of MCD or MPT, whereas the protein still contained stoichiometric amounts of cytidine moieties, like CDP, dCDP, CMP, dCMP, CTP, or dCTP (28). These observations can now be explained by our results with MocA.…”
Section: Discussionsupporting
confidence: 83%
See 1 more Smart Citation
“…It has been reported previously that cell extracts from H. pseudoflava grown in the absence of molybdate did not produce MCD (28). In addition, CO dehydrogenase expressed under these conditions was devoid of MCD or MPT, whereas the protein still contained stoichiometric amounts of cytidine moieties, like CDP, dCDP, CMP, dCMP, CTP, or dCTP (28). These observations can now be explained by our results with MocA.…”
Section: Discussionsupporting
confidence: 83%
“…Another well characterized MCD-containing enzyme is CO dehydrogenase from Hydrogenophaga pseudoflava. It has been reported previously that cell extracts from H. pseudoflava grown in the absence of molybdate did not produce MCD (28). In addition, CO dehydrogenase expressed under these conditions was devoid of MCD or MPT, whereas the protein still contained stoichiometric amounts of cytidine moieties, like CDP, dCDP, CMP, dCMP, CTP, or dCTP (28).…”
Section: Discussionmentioning
confidence: 74%
“…The hypothetical MobA protein for the molybdopterin cytidine dinucleotide cofactor is encoded by an ORF which may form a transcriptional unit with ORFs of no known functions but that are presumed to be involved in the assembly of the MoCo holoenzymes, attachment of the enzymes to the cell membrane, and interaction with the respiratory chain (18,28). MobA could deliver, in the context of these proteins, the molybdopterin cytidine dinucleotide cofactor efficiently to the membrane-associated KDH and NDH apoenzymes (16).…”
Section: Vol 183 2001mentioning
confidence: 99%
“…Nicotine, the alkaloid of the tobacco plant, is synthesized as the L-isomer, and the first enzyme to attack L-nicotine is a trimeric, molybdopterin cofactor (MoCo) (most probably in its dinucleotide form [18])-, flavin adenine dinucleotide (FAD)-, and [Fe-S] clustercontaining nicotine dehydrogenase (NDH), which hydroxylates the pyridine ring at position 6 (Fig. …”
mentioning
confidence: 99%
“…The enzyme is an O 2 -stable, molybdenum-iron-sulfurflavin hydroxylase that catalyzes the oxidation of CO to CO 2 according to the equation CO ϩ H 2 O 3 CO 2 ϩ 2e Ϫ ϩ 2H ϩ . It contains the molybdopterin cytosine dinucleotide-type molybdenum cofactor (29) and [2Fe-2S] centers of type I and type II (2,10). In anaerobic microorganisms, CODHs are nickeliron-sulfur proteins, usually O 2 labile, that function in a variety of energy-yielding pathways.…”
mentioning
confidence: 99%