Effect of mono‐(2‐ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro

Li, Na; Liu, Te; Guo, Kun; Zhu, Jian; Yu, Ge; Wang, Shuyue; Ye, Lin

doi:10.1002/jcp.26224

Cited by 33 publications

(15 citation statements)

References 41 publications

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“…The expressions of StAR and P450scc were lower after exposure to MnBP (Figure 6). These results are similar to those of the exposure of MEHP in rat ovarian granulosa cells and the exposure of DBP in human granulosa cells at low dose [45,48].…”

Section: Effect Of Mnbp On Amh Inhibin B Star and P450ssc Expressionsupporting

confidence: 86%

“…Several reports have shown that phthalate metabolites may affect the granulosa cells. In rat granulosa cells, MEHP suppresses aromatase transcript levels, estradiol production, steroid hormone synthesis, and key enzymes in progesterone production, and causes the attenuation of cell proliferation and induction of apoptosis [16,44,45]. These results suggest that MEHP has important effects on rodent granulosa cells, but there is no information in humans.…”

Section: Introductionmentioning

confidence: 98%

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Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells

Chou

Chen

et al. 2020

IJMS

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To study the association between urinary phthalate metabolite levels, endometriosis, and their effects on human granulosa cells, we recruited patients who underwent laparoscopy to confirm endometriosis (n = 123) and control patients (n = 78). Liquid chromatography–tandem mass spectrometry was used to measure the following five urinary phthalate metabolites: mono-n-butyl phthalate (MnBP), mono(2-ethylhexyl) phthalate, monobenzyl phthalate, mono(2-ethyl-5-oxo-hexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate. Urinary MnBP levels were higher in patients with endometriosis than in controls after multivariable logistic regression including the number of deliveries, body mass index, and use of medicine as covariables. MnBP correlates with other phthalate metabolites. Previous studies found that endometriosis was a detrimental condition for granulosa cells. In our study, we observed whether MnBP affected granulosa cells. MnBP treatment altered the gene expression of BIRC5, BUB1B, CDC20, cyclin B1, IL-1β, TNF-α, inhibin-B, StAR, and P450ssc and attenuated the ratio of the mitochondrial membrane potential in human granulosa cells. Moreover, MnBP decreased the expression of the anti-Mullerian hormone. These findings suggest that MnBP concentration is associated with endometriosis and may affect the health and steroidogenesis of human granulosa cells.

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Section: Effect Of Mnbp On Amh Inhibin B Star and P450ssc Expressionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 98%

Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells

Chou

Chen

et al. 2020

IJMS

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“…A number of studies have shown that environmental endocrine-disrupting chemicals (EDCs) can affect steroidogenesis in GCs [24,25]. Bisphenol A (BPA), a chemical widely used for the production of epoxy resins and polycarbonate plastics, was found to be an important environmental EDC.…”

Section: Research Highlightmentioning

confidence: 99%

Downregulation of gap junctional intercellular communication and connexin 43 expression by bisphenol A in human granulosa cells

Lin

Wang

Chuang

et al. 2020

Biotech and App Biochem

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Gap junctional intercellular communication (GJIC) is the transfer of ions, metabolites, and second messengers between neighboring cells through intercellular junctions. Connexin 43 (Cx43) was found to be the type of gap junction protein responsible for human granulosa cells (GCs) and oocyte communication, which is required for folliculogenesis and oocyte maturation. Bisphenol A (BPA), an estrogenic-like endocrine-disrupting chemical, is one of the most widely produced chemicals around the world. There are reports that the chemical might cause endometrial tumorigenesis and several female reproductive disorders. This study demonstrated that cell culture medium, containing antioxidants (N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate), was able to enhance the survival and self-renewal of GCs. In addition, we found that BPA at environmentally relevant concentration (10 −7 M) reduced Cx43 expression and GJIC in GCs through estrogen receptor and mitogen-activated protein kinase pathways. The results of this study not only reveal the reproductive toxicity of BPA but also provide possible mechanisms by which BPA inhibited GJIC in GCs.

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“…It induced ovarian toxicity by inhibition of follicular development and abnormal steroid hormone synthesis in cultured rat ovarian follicles 85 . It also inhibited the rat granulosa cell viability, increased apoptosis through caspase‐3 activation and Bcl‐2‐associated x protein (BAX) expression, stimulated steroid hormone secretion, and induced the expression of key enzymes in progesterone expression and sex hormone receptors 86‐88 …”

Section: Effects Of Phthalate On Granulosa Cellsmentioning

confidence: 99%

The impact of phthalate on reproductive function in women with endometriosis

Chou

Tzeng

2021

Reprod Medicine & Biology

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Background Endometriosis is a common gynecological condition in which stromal or glandular epithelium is implanted in extrauterine locations. Endometriosis causes detrimental effects on the granulosa cells, and phthalate interferes with the biological and reproductive function of endometrial cells at a molecular level. Methods This article retrospectively reviewed the studies on phthalate exposure and its relationship with endometriosis. A literature search was performed for scientific articles using the keywords “phthalate and endometriosis,” “endometriosis and granulosa cells,” “phthalate and granulosa cells,” and “phthalates and endometrial cells.” Results Endometriosis can affect cytokine production, steroidogenesis, cell cycle progression, expression of estrogen receptor‐α (ER‐α)/progesterone receptor (PR), and cause endoplasmic reticulum stress, senescence, apoptosis, autophagy, and oxidative stress in the granulosa cells. Mono‐n‐butyl phthalate (MnBP) alters the expression of cytokines, cell cycle‐associated genes, ovarian stimulation, steroidogenesis, and progesterone production. Several in vitro studies have demonstrated that phthalate caused inflammation, invasion, change in cytokines, increased oxidative stress, viability, resistance to hydrogen peroxide, and proliferation of endometrial cells. Conclusion This might provide new insights about the impact of phthalate on the pathogenesis of endometriosis and its consequences on the ovarian function.

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Effect of mono‐(2‐ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro

Cited by 33 publications

References 41 publications

Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells

Exposure to Mono-n-Butyl Phthalate in Women with Endometriosis and Its Association with the Biological Effects on Human Granulosa Cells

Downregulation of gap junctional intercellular communication and connexin 43 expression by bisphenol A in human granulosa cells

The impact of phthalate on reproductive function in women with endometriosis

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