1982
DOI: 10.1128/jvi.41.2.478-488.1982
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Effect of monoclonal antibodies on limited proteolysis of native glycoprotein gD of herpes simplex virus type 1

Abstract: We examined the properties of 17 monoclonal antibodies to glycoprotein gD of herpes simplex type 1 (HSV-1) (gD-1) and HSV-2 (gD-2). The antibodies recognized eight separate determinants of gD, based on differences in radioimmuno-precipitation and neutralization assays. The determinants were distributed as follows: three were gD-1 specific, one was gD-2 specific, and four were type common. Several type-specific and type-common determinants appeared to be involved in neutralization. We developed a procedure for … Show more

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Cited by 89 publications
(114 citation statements)
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“…Analysis of the glycopeptides of gD-1 and gD-2 by cation-exchange chromatography. We previously characterized the tryptic peptides of gD-1 and gD-2 labeled with either [35S]methionine or [3H]arginine by cation-exchange chromatography on Chromabeads P (6, [8][9][10][11]26). [3H]mannoseand [35S]methionine-labeled tryp-MBER tic peptides of pgD-1 ( Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Analysis of the glycopeptides of gD-1 and gD-2 by cation-exchange chromatography. We previously characterized the tryptic peptides of gD-1 and gD-2 labeled with either [35S]methionine or [3H]arginine by cation-exchange chromatography on Chromabeads P (6, [8][9][10][11]26). [3H]mannoseand [35S]methionine-labeled tryp-MBER tic peptides of pgD-1 ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…This was further evidence that pgD-1 contained N-asparagine-linked oligosaccharides. From studies of the nature and size of the oligosaccharides found in the precursor and product forms of gD-1; we previously proposed that this glycoprotein contained from one to three N-asparagine-linked oligosaccharides (9,26). By examining similar details in gD-2 (10) and comparing it with gD-1, we further speculated that gD-2 might contain fewer oligosaccharide residues than does gD-1.…”
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confidence: 95%
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“…Mice immunized with purified gD from HSV-1 or HSV-2 are protected against lethal challenge with virus (131). The antigenic regions on gD have been extensively studied and mapped in detail (52,54,161,164). A site between amino acids 234 and 244 is essential for 332 infectivity, but has only marginal antigenicity (165).…”
Section: 13mentioning
confidence: 99%
“…Immunization of mice with gD of HSV-1 results in high neutralizing-antibody titers against HSV-1, and these mice are protected against a lethal HSV challenge [ 2 ] . mAbs directed against different regions of gD of HSV-1 have been divided into groups according to their antigenic sites [3], mAbs which recognize amino acid residues 11 -19 in the N-terminus are designated as group VII mAbs [4, 51. mAb A16 is a group VII mAb that binds a synthetic peptide consisting of amino acid residues 9-19 of gD [6,71. Immunization with synthetic peptides comprising this region of HSV gD protected mice against a lethal challenge with HSV [8,91.…”
mentioning
confidence: 99%