Effect of morin on tissue lipid peroxidation and antioxidant status in 1, 2-dimethylhydrazine induced experimental colon carcinogenesis

Abstract

Colon cancer is the third most malignant neoplasm in the world and it remains today an important cause of death, especially in western countries. In this study, we have evaluated the chemopreventive efficacy of morin on tissue lipid peroxidation and antioxidant status, which are used as biomarkers in 1,2-dimethylhydrazine-induced colon carcinogenesis in a rat model. Male Wistar rats were divided into four groups and received high fat diet. Group 1 served as control, groups 2 and 4 were given a daily treatment of morin (50 mg/kg body weight) orally, everyday for a total period of 30 weeks. Groups 3 and 4 were given weekly subcutaneous injections of DMH at a dose of 20 mg/kg body weight in the groin for 15 weeks. Animals were sacrificed at the end of 30 weeks. The liver, intestine, colon and caecum from different groups were subjected to histopathological studies, determination of lipid peroxidation and antioxidant status. Our results showed decreased levels of liver enzymic and non-enzymic antioxidants and increased levels of lipid peroxidation (LPO) products such as tissue thiobarbituricacid substances (TBARS), lipid hydroperoxides (LOOH) and conjugated dienes (CD) in DMH treated rats, which were significantly (P < 0.05) reversed on morin supplementation. Moreover, intestinal, colonic and caecal TBARS, LOOH, CD and also the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) were significantly diminished in DMH treated rats, which were significantly (P < 0.05) elevated on simultaneous morin supplementation. Moreover, enhanced activity of intestinal, colonic and caecal ascorbic acid and alpha-tocopherol levels were also observed in DMH alone treated rats, which were significantly (P < 0.05) reduced on morin supplementation. These results indicate that morin could exert a significant chemopreventive effect on colon carcinogenesis induced by DMH.

Morin, A Flavonoid, On Lipid Peroxidation And Antioxidant Status In Experimental Myocardial Ischemic Rats

“…By protecting myocardial membranes and inhibiting the oxidation of lipoproteins, vitamin E inhibits membrane peroxidative damage and atherogenesis (Upston et al, 1999). Ceruloplasmin inhibits ferritin-dependent lipid peroxidation by catalyzing the oxidative reincorporation of released irons into ferritin (Sreedharan et al, 2009). Pretreatment with morin to ISO-induced rats significantly increased the levels of vitamin C and E in both plasma and heart and ceruloplasmin levels in plasma.…”

Oxidative Stress and Antioxidant Status in Acute and Chronic Myeloid Leukemia Patients

“…GR is a glutathione regenerating enzyme that permits the conversion of oxidized glutathione (GSSG) to reduced glutathione (GSH) by the oxidation of NADH to NAD + [21]. Glutathione reductase is a secondary antioxidant enzyme that helps in the detoxification of reactive oxygen species by decreasing peroxide levels or by maintaining a steady state supply of metabolic intermediates like GSH [22].…”

Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study

“…Morin was freshly suspended in water [26,27] and was administered to rats orally using an intragastric tube daily for a period of six consecutive weeks. Rats were monitored daily for general health.…”