Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Abstract

Introduction: Aluminium, a ubiquitous metal implicated in some neurodegenerative diseases is linked to activation of free oxygen species. The antioxidant-rich plants, Moringa oleifera (MO) is reported to protect against Aluminium activities. This study investigated the actions of MO leaf extract (MOLE) against Aluminium chloride (AlCl3 )- induced hippocampal cellular changes and serum levels of alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT) in adult Wistar rats. Materials and Methods: Thirty Wistar rats weighing between 150 g and 220 g were grouped (n=5) into; 1-control (5 mL/kg distilled water), 2-AlCl3 (100 mg/kg), 3-low dose MOLE (250 mg/kg), 4-high dose MOLE (1,000 mg/kg), 5-concurrent AlCl3 and low dose MOLE, and 6-concurrent AlCl3 and high dose MOLE. All administrations were by oral gavages for 21 days. On day 22, following deep anaesthesia and cardiac puncture, blood was obtained for serum enzyme analysis, and the brain perfusion fixed, harvested and processed for histological study. Results: Results showed significantly (p < 0.05) higher ALP level in the AlCl3 group compared with the control, as well as the other test groups. However, there was no significant (p > 0.05) AST and ALT levels. The hippocampal CA3 of the AlCl3 group showed hypertrophic cells, with some of the cells having karyorrhectic features. The concurrent AlCl3 and low and high doses, MOLE groups showed less of these adverse features. Conclusion: These results suggest that MOLE may protect enzymatic activities against Aluminium chloride. However, its action on hippocampus is still subject to further investigation.

Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves

“…Some studies have confirmed that Moringa oleifera has a neuroprotective effect [84][85][86][87][88]; as far as we know, nevertheless, no studies have yet described and determined the potential effective neuroprotection components. Quercetin and kaempferol derivatives are the main polyphenols in Moringa oleifera leaves.…”

Cognitive, Anticholinesterase and Anti-Oxidative Potentials of Zingiber officinale Rhizome Extracts against Aluminium Chloride- Induced Neurotoxicity in Swiss Mice

“…The remaining forty-eight mice were equally divided into eight groups (n = 6), and were orally administered AlCl 3 at 100 mg/kg body weight (Ekong et al 2017;Adighije et al 2020), and Z. officinale ethanol, dichloromethane (DCM), and methanol extracts daily for 21 days: Group 1 were the control administered 40 mL/kg of distilled water daily; group 2 were administered 100 mg/kg AlCl 3 only; group 3 were concurrently administered 100 mg/kg AlCl 3 and 2.5 mg/kg of donepezil; while groups 4-6 were concurrently administered 100 mg/kg AlCl 3 and 474 mg/kg (10% LD 50 low dose), 949 mg/kg (20% LD 50 intermediate dose), and 1,423 mg/kg (30% LD 50 high dose) Z. officinale ethanol extract; groups 7 and 8 were concurrently administered 100 mg/kg AlCl 3 and 949 mg/kg DCM or 949 mg/kg methanol fractions of Z. officinale.…”