Effect of Morphology of Conducting Polymer on DNA Sensing

Abstract

Electrochemical DNA sensors can be constructed by understanding basic interfacial electron transfer between solid surface-electrolyte-DNA interfaces. The kinetics of this heterogeneous process can be significantly affected by the microstructure and roughness of the electrode surface. By understanding this concept, in this paper; we compared the performance of micro electrodes containing poly(Py-co-PAA) with macro electrode containing same copolymer, showing that micro electrodes are more sensitive than the macro electrodes for biosensor applications. Sensors based on the copolymer electropolymerised on both micro and macro electrodes were evaluated across a range of oligonucleotide concentrations. The interfacial electron charge transfer resistance between the solution and electrode surface was studied using electrochemical impedance spectroscopy (EIS).