Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model

Sliepen, I.; Essche, Mark Van; Quirynen, Marc; Teughels, Wim

doi:10.1007/s00784-009-0286-0

Cited by 17 publications

(10 citation statements)

References 51 publications

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“…There is no doubt that Listerine® has potent antimicrobial activity against numerous microorganisms (Kubert et al , , Yamanaka et al , , Kasuga et al , , Okuda et al , ). However, in experimental biofilms, pathogenic microorganisms found in root caries were less affected (Zheng and Wang, , Smith et al , , Sliepen et al , ). Our results confirm that microorganisms have different sensitivities to the five‐component‐mixture Listerine® (Kato et al , , Haffajee et al , ), as well as against the Listerine® phenolic components, their mixtures, and ethanol.…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Investigation on the Antimicrobial Activity of Listerine®, Its Components, and of Mixtures Thereof

et al. 2015

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Listerine® is one of the most popular mouthwashes worldwide and claims to combat harmful bacteria. In the past century, its recipe was changed from an essential oil mouthwash to a five-component mixture (thymol, menthol, eucalyptol, and methyl salicylate dissolved in 27% ethanol). The aim of this study was to get preliminary information about the antimicrobial activities of individual Listerine® components and their mixtures. We tested the bacterial strains Streptococcus mutans, Enterococcus faecalis, and Eikenella corrodens and the yeast Candida albicans. The established minimum inhibitory concentration (MIC) assay and the minimum bactericidal/fungicidal concentration (MBC/MFC) assay were applied. None of the combinations of two phenols at the concentrations contained within Listerine® were associated with either an additive or synergistic effect. Thymol had lower MIC and MBC/MFC values than the other Listerine® components and Listerine® against E. corrodens and C. albicans. The mixtures consisting of eucalyptol, methyl salicylate, and thymol were the most effective against S. mutans and E. faecalis and more effective than Listerine®. Our results demonstrate that the phenols and their concentrations as contained within Listerine® could be further optimized in terms of selecting those which increase their general effectiveness, at concentrations that do not induce harm.

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Section: Discussionmentioning

confidence: 99%

A Preliminary Investigation on the Antimicrobial Activity of Listerine®, Its Components, and of Mixtures Thereof

et al. 2015

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“…Several studies have attempted to study the effect of mouthrinses in biofilms in vitro (8-12). Traditionally, bacterial counts on agar plates was the method of choice for determination of bacterial viability, although this method has clear limitations, as the relatively long times needed for the colony growth, the differences in the growth media used or the likely growth inhibition by neighbouring cells (13).…”

Section: Introductionmentioning

confidence: 99%

Validation of ATP bioluminescence as a tool to assess antimicrobial effects of mouthrinses in an in vitro subgingival-biofilm model

Sánchez¹,

Llama‐Palacios²,

Marín³

et al. 2013

Med Oral

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Objectives. The aim of this investigation was to evaluate whether the adenosine triphosphate (ATP) bioluminescence method is an appropriate tool to assess the efficacy of antiseptic mouthrinses in terms of quantitative reductions of total viable microbial counts in mixed biofilm populations in vitro. Study Design. Three mouthrinses, containing respectively, chlorhexidine and cetylpyridinium chloride (CHX/CPC), essential oils (EO) and amine fluoride/stannous fluoride (AFSF), as well as Phosphate Buffered Saline (PBS) used as control, were tested in an in vitro static biofilm model by ATP bioluminescence and compared to culture method. Biofilms were grown on saliva-coated hydroxyapatite disks for 72 hours and then exposed for 1 minute to the mouthrinse or control by immersion. The antibacterial effect of the rinses was tested by analysis of variance. The reliability of the ATP bioluminescence method was assessed by calculating the Pearson correlation coefficients when compared to the viable cell counts obtained by culture. Results. Using ATP bioluminescence, the antimicrobial activity of the tested mouthrinses was demonstrated when compared to the PBS control. The ATP bioluminescence values were significantly correlated (0.769, p<0.001) to the viable cell counts. CHX/CPC and AFSF showed similar antimicrobial activity, although AFSF had a less homogeneous effect, being both more effective than the EO rinse. Conclusion. ATP bioluminescence viability testing may be considered a useful tool to assess the in vitro efficacy of antibacterial compounds. In the proposed model, CHX/CPC and AFSF containing mouthrinses demonstrated superior antimicrobial activity, as compared to EO rinses, in a multispecies biofilm model. Key words:Biofilm, ATP bioluminescence,mouthrinse, essential oils, chlorhexidine, amine fluoride/stannous fluoride.

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“…It exists in three different concentrations (0.20%, 0.12%, and 0.05%) related to its clinical use. For this analysis, the percentage of CHX chosen is 0.12%, that is, the concentration commonly used for biofilm modulation in long-term therapies [12, 15, 16]. Its antibacterial activity is linked to the capability of binding to bacterial cell membrane.…”

Section: Introductionmentioning

confidence: 99%

“…Its antibacterial activity is linked to the capability of binding to bacterial cell membrane. This determines an increased permeability at low concentration while, at high concentration, it causes a precipitation of bacterial cytoplasm with bactericidal effect [15, 16]. …”

Section: Introductionmentioning

confidence: 99%

Oil Essential Mouthwashes Antibacterial Activity againstAggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects

Erriu

Pili

Tuveri

et al. 2013

International Journal of Dentistry

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The aim of this work is to determine the antibacterial activity of three marketed mouthwashes on suspended and sessile states of Aggregatibacter actinomycetemcomitans. The efficacy of two commonly used products in clinical practice, containing essential oils as active ingredients (menthol, thymol, methyl salicylate, and eucalyptol) in association with or without alcohol, has been evaluated in comparison with a chlorhexidine-based mouthwash. The microtiter plate assay, in order to obtain a spectrophotometric measurement of bacterial responses at growing dilutions of each antiseptic, was used for the study. The analysis revealed that a good antibacterial activity is reached when the abovementioned mouthwashes were used at concentration over a 1/24 dilution and after an exposure time of 30 seconds at least. In conclusion, the alcoholic mouthwash appears to have a better biofilm inhibition than its antiplanktonic activity while the nonalcoholic product demonstrates an opposite effect with a better antiplanktonic behavior.

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Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model

Cited by 17 publications

References 51 publications

A Preliminary Investigation on the Antimicrobial Activity of Listerine®, Its Components, and of Mixtures Thereof

A Preliminary Investigation on the Antimicrobial Activity of Listerine®, Its Components, and of Mixtures Thereof

Validation of ATP bioluminescence as a tool to assess antimicrobial effects of mouthrinses in an in vitro subgingival-biofilm model

Oil Essential Mouthwashes Antibacterial Activity againstAggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects

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