Effect of mTHPC-mediated photodynamic therapy on 5-fluorouracil resistant human colorectal cancer cells

Abstract

The primary or acquired cancer chemoresistance is a major problem in the treatment of cancer patients. It could be overcome by non-overlapping treatment regimens such as photodynamic therapy (PDT). PDT is based on the oxidation of cellular components which occurs when a light-excited photosensitizer generates reactive oxygen species (ROS). In this study the effect of mTHPC mediated PDT (mTHPC-PDT) on 5-FU resistant colorectal cancer (CRC) cells HCT116 was investigated. The results show that mTHPC-PDT overcomes 5-FU resistance and is effective against chemoresistant colorectal carcinoma cells.

Photodynamic therapy as salvage therapy for residual microscopic cancer after ultra-low anterior resection: A case report

“…First, there is resistance to chemotherapy drugs. Studies have found that almost all CRC patients develop resistance to chemotherapy[16]. Second, chemotherapy drugs produce treatment toxicity, including mild reactions such as nausea, vomiting, and hair loss and serious reactions such as liver and kidney failure.…”

“…First, the photosensitizer accumulates in cancer cells, and then induces ROS formation via stimulation by light irradiation. Since photosensitizers are only locally deposited and activated in cancer cells, leading to tumour cell death through ROS effects, PDT has a targeted effect and does little harm to normal cells[16]. The greatest advantage of PDT, in addition to its high selectivity to tumours, is the lack of cross-resistance.…”

“…The greatest advantage of PDT, in addition to its high selectivity to tumours, is the lack of cross-resistance. A large amount of preclinical evidence shows that PDT can not only overcome drug resistance, but even make drug-resistant cells sensitive again[16,18]. Additionally, PDT has other advantages over traditional therapy.…”

The role of photodynamic therapy on multidrug resistant breast cancer

“…Chen and colleagues [85] also suggested that DTP-PDT could exhibit inhibition of MDR1 gene expression at molecular level with an important realistic significance. More also, Kukcinaviciute et al [86] demonstrated the usefulness of mTHPC-mediated PDT on 5-fluorouracil resistant human colorectal cancer cells. These studies highlights the role of PDT in chemo-resistance reversal and potentiation in MDR.…”

Photodynamic diagnosis and photodynamic therapy of colorectal cancer in vitro and in vivo

“…68 Several other studies have also gone on to report signicant cellular morphology, viability, proliferation and cytotoxicity changes in CRC cell lines and tumors, which received m-THPC mediated PDT, however also noted the induction of possible pro-survival autophagy cell death pathways and PDT resistance mechanisms. [69][70][71] In order to overcome chlorin PS inabilities to be used in PDD applications, studies by Shimizu et al (2018) developed talaporn sodium (TS: mono-laspartyl chlorine e6, or NPe6), which a second-generation chlorin derivative PS, that has absorption peaks in the Soret band of 398 nm and Q bands of 502, 530, 620 and 654 nm. 72 Thus, TS can emit illumination upon activation within its Soret band, as well as generate ROS and singlet oxygen when activated within its Q-band region.…”