Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm

Wei, Guoxian; Campagna, Alexander N; Bobek, Libuse A.

doi:10.1093/jac/dkl120

Cited by 192 publications

(161 citation statements)

References 33 publications

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“…After drying, 95 % ethanol was added to the wells and the A 595 of stained adherent bacteria was measured with an Emax microtitre ELISA reader (Molecular Devices). The percentage of biofilm inhibition was calculated using the equation: [12(A 595 of cells treated with nano-Ags/A 595 of non-treated control)]6100 (Wei et al, 2006). Experiments were performed in triplicate, and means±SD were determined.…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Hwang

Choi

et al. 2012

Journal of Medical Microbiology

242

146

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Silver nanoparticles (nano-Ags), which have well-known antimicrobial properties, are used extensively in various medical and general applications. In this study, the combination effects between nano-Ags and the conventional antibiotics ampicillin, chloramphenicol and kanamycin against various pathogenic bacteria were investigated. The MIC and fractional inhibitory concentration index (FICI) were determined to confirm antibacterial susceptibility and synergistic effects. The results showed that nano-Ags possessed antibacterial effects and synergistic activities. The antibiofilm activities of nano-Ags alone or in combination with antibiotics were also investigated. Formation of biofilm is associated with resistance to antimicrobial agents and chronic bacterial infections. The results indicated that nano-Ags also had antibiofilm activities. To understand these effects of nano-Ags, an ATPase inhibitor assay, permeability assay and hydroxyl radical assay were conducted. The antibacterial activity of nano-Ags was influenced by ATPassociated metabolism rather than by the permeability of the outer membrane. Additionally, nanoAgs generated hydroxyl radicals, a highly reactive oxygen species induced by bactericidal agents. It was concluded that nano-Ags have potential as a combination therapeutic agent for the treatment of infectious diseases by bacteria.

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Section: Methodsmentioning

confidence: 99%

Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Hwang

Choi

et al. 2012

Journal of Medical Microbiology

242

146

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show abstract

“…After forming biofilms of S. mutans at the base of 96 well plates and then replacing the medium with buffer solution, samples were irradiated with the plasma. Subsequently, the activity of living bacteria was detected by changes in the fluorescence level emitted by a redox indicator 14) . After irradiating the cultures for 2, 3 and 5 minutes at pH4.5, the microbial activity was observed to have significantly decreased (p<0.001) (Fig.…”

Section: Test For Bactericidal Effects On the Biofilm In The Liquid Pmentioning

confidence: 99%

Microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens

et al. 2011

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Research using low frequency atmospheric pressure plasma jets (LF jet) is becoming increasingly more common. We carried out experiments to evaluate the sterilizing effects of this technology on oral pathogenic microorganisms (S.mutans, C.albicans and E. faecalis) and to determine its potential for clinical application. We performed the direct exposure test on a solid surface, indirect exposure test on a liquid phase, and ROS (reactive oxygen species) inhibitory test. The results showed the LF jet had microbicidal effects on oral pathogens, and that the ROS influenced this sterilization effect. The experiments of this study revealed that LF jet had a sterilizing effect on oral pathogenic microorganisms present in both the solid and liquid phases. The sterilizing mechanism was considered to be related to the effect of superoxide anion radicals. These results indicate that LF jets may represent a novel technology that can be applied to the field of clinical dentistry.

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“…Another factor is that biofilm formation inhibition may also inhibit bacterial growth when biofilm formation and bacterial growth are evaluated simultaneously (Wei et al, 2006), as in the case of this work. According to Khemaleelakul et al (2006), microorganisms residing in biofilms are protected against the toxic effect of antimicrobials via inactivation by enzymes and other metabolites that degrade or neutralize the antimicrobial agent.…”

Section: Discussionmentioning

confidence: 99%

“…The minimum inhibitory concentration of biofilm (MICB50) is defined as the minimum antibacterial agent concentration that is able to inhibit 50% or more of biofilm formation (Wei et al, 2006). MICB50 was determined as described in international guidelines (CLSI, 2007) with some modifications.…”

Section: Biofilm Formation Inhibition As Assessed By Minimum Inhibitomentioning

confidence: 99%

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Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

Marangoni¹,

Moraes²,

Utrera³

et al. 2018

J. Pharmacognosy Phytother.

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Viguiera arenaria, family Asteraceae, is a plant that contains diterpenoids, which make this species potentially applicable in endodontics. More specifically, V. arenaria contains diterpenes of the pimarane type, which display various classic biological activities. This study evaluates the antibiofilm activity, the time-kill curve, and the inhibitory concentration index of diterpenes of the pimarane type (entpimara-8(14),15-dien-19-oic acid, ent-8(14),15-pimaradien-3β-ol, and ent-8(14),15-pimaradien-3β-19-oic acid sodium salt, designated diterpenes I, II, and III, respectively) toward nine anaerobic bacteria commonly found in endodontic infections; this study also assesses the cytotoxic activity of these diterpenes against human fibroblasts. According to the antibiofilm assay, diterpenes I, II, and III inhibit at least 50% of all the bacteria. On the basis of the time-kill curve experiments, the behavior of these diterpenes depends on the tested bacteria, diterpene concentration, and microorganism sensitivity. Synergism of diterpenes I and II with chlorhexidine (CDH) was higher against P. gingivalis (clinical isolate) and Aggregatibacter actinomycetemcomitans (ATCC). As for diterpene III, synergism with CDH is higher against P. micros. As revealed by the XTT assay, none of the diterpenes of the pimarane type tested here are cytotoxic. Hence, diterpenes I, II, and III are promising biomolecules and may provide therapeutic solutions in the field of endodontics.

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Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm

Abstract: MUC7 peptides present somewhat preferential antimicrobial activity against S. mutans. They also have an effect on in vitro formation and reduction of the preformed S. mutans biofilm.

Cited by 192 publications

References 33 publications

Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

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