Effect of multiplicity of infection on the evasion of neutrophil killing by Streptococcus agalactiae isolated from clinical mastitis bovine

Ma, Feifei; Yang, Shifang; Wang, Guangyu; Zhou, Mingxu; Zhang, Jinqiu; Deng, Bihuang; Yin, Wenzhu; Wang, Haiyan; Lu, Yu; Fan, Hongjie

doi:10.1016/j.vetmic.2022.109450

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Bovine neutrophils displayed minimal apoptotic phenotype expression upon exposure to minor mastitis bacteria, aligning with flow cytometry data and suggesting little effect on apoptosis induction [ 15 ]. However, a recent report suggested that the dosage used for in vitro bacterial infection can significantly suppress neutrophil functions and cell death [ 22 ]. Though using an in vitro MOI of 10 aligns with prior studies, future experiments should explore diverse dosages for a more definitive understanding of how minor mastitis bacterial exposure impacts apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…The downregulation of BAX by cells exposed to either S. chromogenes or W. paramesenteroides , despite the upregulation of other pro-apoptotic genes, indicates additional regulatory roles beyond the Bcl-2 family in bovine neutrophil apoptosis. The ability to manipulate apoptosis within bacteria-harboring neutrophils could be essential for maintaining the cellular longevity, viability and functionality of neutrophils, enabling them to effectively combat these sustained challenges [ 22 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Divergent Immune Responses to Minor Bovine Mastitis-Causing Pathogens

Srithanasuwan,

Pangprasit,

Mektrirat

et al. 2024

Veterinary Sciences

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Traditionally, non-aureus staphylococci and mammaliicocci (NASM) were not considered significant players in bovine mastitis. This study investigated the involvement of NASM (Staphylococcus hominis and Staphylococcus chromogenes) and lactic acid bacteria (LAB) strains (Weissella paramesenteroides) through bovine neutrophil responses. Bovine neutrophils displayed minimal apoptosis upon NASM and LAB challenge. Neutrophils expressed high TLR2 after challenge, but TLR6 expression varied and remained low in NASM pathogen recognition. Bovine neutrophils effectively engulfed and killed LAB, but their activity was significantly impaired against NASM. This was evident in S. chromogenes, where reduced TLR6 recognition and a weakened phagocytic response likely contributed to a lower bactericidal effect. Regardless of the bacteria encountered, intracellular ROS production remained high. S. chromogenes-challenged neutrophils displayed upregulation in genes for pathogen recognition (TLRs), ROS production, and both pro- and anti-apoptotic pathways. This response mirrored that of Weissella. except for CASP9 and BCL2, suggesting these bacteria have divergent roles in triggering cell death. Our findings suggest that S. chromogenes manipulates bovine neutrophil defenses through coordinated changes in functional responses and gene expression, while LAB strains have a weaker influence on apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Divergent Immune Responses to Minor Bovine Mastitis-Causing Pathogens

Srithanasuwan,

Pangprasit,

Mektrirat

et al. 2024

Veterinary Sciences

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“…As one of the most common diseases in dairy cows, bovine mastitis affects milk production and composition, lowering milk yield and the nutritional quality of milk. 1 Streptococcus agalactiae , also known as group B Streptococcus (GBS), is a contagious pathogen that causes intramammary infections in dairy cows; 2 , 3 this bacterium can internalize into bovine mammary epithelial cells (BMECs). 4 S. agalactiae expresses surface proteins (adhesins), such as fibrinogen (FBG)-binding protein A/B (FbsA/B), streptococcal fibronectin-binding protein A (SfbA), and laminin-binding protein (Lmb), that bind to host extracellular matrix (ECM) proteins, including fibrinogen (FBG), fibronectin (Fn), and laminin (Lm), which initiate the interaction of the bacteria with integrins on the surface of the host cell, allowing the bacteria to internalize into the host cell.…”

Section: Introductionmentioning

confidence: 99%

Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae

Guo,

Ma,

Jia

et al. 2023

iScience

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Exploring the distinct immunological reactions of bovine neutrophils towards major and minor pathogens responsible for mastitis

Srithanasuwan,

Tata,

Tananupak

et al. 2023

International Journal of Veterinary Science and Medicine

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Bovine mastitis is primarily caused by a group of bacteria known as Staphylococcus and Streptococcus . However, additional types of bacteria, such as bovine non-aureus staphylococci and mammaliicocci (NASM) as well as lactic acid bacteria (LAB), are considered minor pathogens and have less impact on cows. Modulating bovine neutrophil activities and gene expressions in response to bacterial stimuli prompted the cells to execute effector functions to combat udder infections. Although neutrophils can manage major mastitis-causing bacteria, this strategy has not been tested against minor pathogens, i.e. NASM, Weissella spp. Our main objective was to investigate how neutrophils interacted with major and minor pathogens during in vitro bacterial stimulation. The results reveal that neutrophils performed offensive duties regardless of the type of bacteria encountered. Neutrophils generated high levels of reactive oxygen species, efficiently phagocytosed both types of bacteria, and facilitated extracellular killing by releasing NET structures against all bacteria. In addition, neutrophils migrated preferentially towards the majors rather than the minors, although myeloperoxidase (MPO) degranulation did not differ substantially across bacteria. Furthermore, the killing capacity of neutrophils was not dependent on any particular bacterium. The correlation of effector functions is intimately linked to the up-regulation of genes associated with the above functions, except for IL6 , which was down-regulated. Furthermore, neutrophil apoptosis can be modulated by altering apoptosis-associated genes in response to harmful stimuli. These findings provide valuable information on how neutrophils react to major and minor mastitis-causing bacteria. However, future research should explore the interplay between minor pathogens and the host’s responses.

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Effect of multiplicity of infection on the evasion of neutrophil killing by Streptococcus agalactiae isolated from clinical mastitis bovine

Cited by 4 publications

References 37 publications

Divergent Immune Responses to Minor Bovine Mastitis-Causing Pathogens

Divergent Immune Responses to Minor Bovine Mastitis-Causing Pathogens

Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae

Exploring the distinct immunological reactions of bovine neutrophils towards major and minor pathogens responsible for mastitis

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