Effect of Mutacin Administration on Streptococcus mutans‐Induced Dental Caries in Rats

Ooshima, Takashi; Yasufuku, Y; Izumitani, A; Sumi, Norio; Iwanami, Takefumi

doi:10.1111/j.1348-0421.1985.tb00906.x

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…The nature of bacteriostatic substance may not be suitable for the application for the prevention of pneumococcal diseases, directly. Ooshima et al, reported that the mutacin, which was the bacteriocin produced by S. mutans, completely killed the cells grown in sucrose-free medium, whereas the bacteriocin did not kill the S. mutans cell grown in a sucrose-containing medium [17]. The inhibitory mechanism of the bacterioci-like substance in this study may be different from that of other known bacteriocins.…”

Section: Discussioncontrasting

confidence: 55%

Characterization and purification of a bacteriocin-like substance produced by Lactobacillus crispatus LBS 17-11 isolated from an oral cavity of human subject

Akimaru,

Imai,

Sogabe

et al. 2017

Asian Pac J Dent

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Purpose: Lactobacillus crispatus LBS 17-11 was isolated from an oral cavity of human subject, and possess growth inhibition activity against Streptococcus pneumoniae. The objective of this study was to purify a bacteriocin-like substance, and to determine its characteristic properties. Materials and Methods: Antibacterial activities against S. pneumoniae ATCC 33400 during purification steps and against several oral bacteria were determined by a radial diffusion assay. Purification procedure for bacteriocin-like substance was achieved with ammonium sulfate precipitation of culture supernatant, and subsequent extraction with chloroform. Minimum inhibitory concentration (MIC) was determined by liquid microdilution method. Results: The culture supernatant of L. crispatus LBS 17-11 showed a bacteriostatic activity against S. pneumoniae. This substance was partially purified up to 2045-fold specific activity (unit/mg protein). The inhibitory activity was shown against only S. pneumoniae, but Gram negative species. The antimicrobial activity was heat stable and partially lost by the treatment with proteases (trypsin and proteinase K). The molecular mass of bacteriocin-like substance was estimated to be approximately 7 kDa based on tricine SDS-PAGE. Binary combination of 1/2-MIC level of sodium lactate (56 mM) and the bacteriocin-like substance showed lower MIC level compared to that of the addition of the bacteriocin-like substance alone. Conclusion: Bacteriocin-like substance produced by L. crispatus LBS 17-11 showed bacteriostatic activity against S. pneumoniae. The partially purified bacteriocin-like substance was heat stable and degraded by proteases. Molecular mass was estimated to be approximately 7 kDa.

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Section: Discussioncontrasting

confidence: 55%

Characterization and purification of a bacteriocin-like substance produced by Lactobacillus crispatus LBS 17-11 isolated from an oral cavity of human subject

Akimaru,

Imai,

Sogabe

et al. 2017

Asian Pac J Dent

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“…Differences in the sensitivity spectrum of the indicator strains were also noted among these three mutacins. Notwithstanding the differences in various properties, we now have strong evidence to indicate that bacteriocins, including mutacins, can affect microbial ecology in the oral flora and some bacteriocins may function to suppress caries development if they have the ability to inhibit the growth of mutans streptococci in vitro (Fitzgerald et al, 1986;Ikeda et al, 1982;Ooshima et al, 1985;Weerkamp et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Purification and properties of extracellular mutacin, a bacteriocin from Streptococcus sobrinus

Loyola-Rodríguez

Morisaki²,

Kitamura³

et al. 1992

Journal of General Microbiology

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Mutacin MT6223, a cell-free bacteriocin produced by S?reptococcus sobrinus MT6223, was purified by ammonium sulphate precipitation, chromatofocusing with PBE 94 and column chromatography on SP Sephadex C-25. The specific activity of the purified mutacin was increased 1950-fold with a recovery of 9.7 %. The molecular mass of the purified mutacin preparation was estimated to be 6-5 kDa. The mutacin activity was stable from pH 2-7, and was resistant to treatment at 100 "C for 20 min. It was inactivated by papain or ficin digestion, and was partially inhibited by a-chymotrypsin. The mutacin was found to be active against strains of serotypes c, e andfof Streptococcus mutans and the addition of purified mutacin MT6223 to growing cells of S. mutans MT8148 resulted in a rapid inhibition of incorporation of 13H]thymidine, [3H]uracil or ~-[~H]glutamic acid into DNA, RNA or protein, respectively. Specific pathogen-free Fisher rats fed diet 2000 and infected with S. mutans MT8148R showed significantly fewer caries and lower plaque scores when mutacin was administered through drinking water. The present study demonstrates that mutacin MT6223 inhibited the growth of mutans streptococci. Thus, mutacin MT6223 may be a candidate for use in dental caries prevention.

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Antibacterial Peptides: Opportunities for the Prevention and Treatment of Dental Caries

Pepperney

Chikindas

2011

Probiotics & Antimicro. Prot.

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Dental caries is a multifactorial disease that is a growing and costly global health concern. The onset of disease is a consequence of an ecological imbalance within the dental plaque biofilm that favors specific acidogenic and aciduric caries pathogens, namely Streptococcus mutans and Streptococcus sobrinus. It is now recognized by the scientific and medical community that it is neither possible nor desirable to totally eliminate dental plaque. Conversely, the chemical biocides most commonly used for caries prevention and treatment indiscriminately attack all plaque microorganisms. These treatments also suffer from other drawbacks such as bad taste, irritability, and staining. Furthermore, the public demand for safe and natural personal hygiene products continues to rise. Therefore, there are opportunities that exist to develop new strategies for the treatment of this disease. As an alternative to conventional antibiotics, antibacterial peptides have been explored greatly over the last three decades for many different therapeutic uses. There are currently tens of hundreds of antibacterial peptides characterized across the evolutionary spectrum, and among these, many demonstrate physical and/or biological properties that may be suitable for a more targeted approach to the selective control or elimination of putative caries pathogens. Additionally, many peptides, such as nisin, are odorless, colorless, and tasteless and do not cause irritation or staining. This review focuses on antibacterial peptides for their potential role in the treatment and prevention of dental caries and suggests candidates that need to be explored further. Practical considerations for the development of antibacterial peptides as oral treatments are also discussed.

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Effect of Mutacin Administration on Streptococcus mutans‐Induced Dental Caries in Rats

Cited by 7 publications

References 18 publications

Characterization and purification of a bacteriocin-like substance produced by <i>Lactobacillus crispatus</i> LBS 17-11 isolated from an oral cavity of human subject

Characterization and purification of a bacteriocin-like substance produced by <i>Lactobacillus crispatus</i> LBS 17-11 isolated from an oral cavity of human subject

Purification and properties of extracellular mutacin, a bacteriocin from Streptococcus sobrinus

Antibacterial Peptides: Opportunities for the Prevention and Treatment of Dental Caries

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