Effect of mutanase and dextranase on biofilms of cariogenic bacteria: A systematic review of in vitro studies

Del Rey, Yumi C.; Parize, Hian; Assar, Sahar; Göstemeyer, Gerd; Schlafer, Sebastian

doi:10.1016/j.bioflm.2024.100202

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Preprint1

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Large-scale screening identifies enzyme combinations that remove in situ grown oral biofilm

Nielsen,

Johnsen,

Hansen

et al. 2024

Biofilm

View full text Add to dashboard Cite

Large-scale screening identifies enzyme combinations that remove in situ grown oral biofilm

Nielsen,

Johnsen,

Hansen

et al. 2024

Biofilm

View full text Add to dashboard Cite

Dental biofilms contain DNase I-resistant Z-DNA and G-quadruplexes

Evans,

Kristensen,

Palmén

et al. 2024

Preprint

View full text Add to dashboard Cite

eDNA is a major component of the extracellular matrix of bacterial biofilms, and recent studies have shown that biofilms from many pathogens contain both Z-DNA and G-quadruplex (G4) structures in addition to the canonical B-DNA double helix. These secondary DNA structures provide new emergent functions to the biofilm, most notably by making biofilms more resilient by protecting them from nucleases. In dental biofilms, it is largely unknown what conformation eDNA adopts, and the aim of this study was to determine if dental biofilms contain non-canonical secondary DNA structures. In this study, we visualised B-DNA, G4, and Z-DNA in dental biofilms from 10 healthy subjects and from 10 caries-active subjects using fluorescence immunolabeling and confocal microscopy. eDNA formed large structures surrounding clusters of microorganisms that contained B-DNA, G4, and Z-DNA in the majority of the samples. We also identified microorganisms outside of these large eDNA structures that contained smaller G4 and Z-DNA structures associated to cell surfaces. G4 and Z-DNA are resistant to degradation by the commonly used mammalian DNase I. We verified this resistance in dental biofilms, and we suggest that these structures present a novel target for improved enzyme formulations for controlling oral biofilms and, more broadly, any biofilm that contains G4 and Z-DNA.

show abstract

Effect of mutanase and dextranase on biofilms of cariogenic bacteria: A systematic review of in vitro studies

Cited by 2 publications

References 65 publications

Large-scale screening identifies enzyme combinations that remove in situ grown oral biofilm

Large-scale screening identifies enzyme combinations that remove in situ grown oral biofilm

Dental biofilms contain DNase I-resistant Z-DNA and G-quadruplexes

Contact Info

Product

Resources

About