Effect of myoblast fusion during muscle cell differentiation on myosin polysome accumulation and myosin synthesis

Cell Differentiation, Protein Synthesis Rate and Protein Accumulation in Muscle Cell Cultures Isolated from Embryos of Layer and Broiler Chickens

“…The significance of this stringent control is emphasized by Young and Dombroske (1981). Embryo extract was prepared as described by Young (1975).…”

Growth characteristics and insulin response of muscle cultures derived from animals with different growth rates in vivo, and localization and accumulation of nebulin during myogenesis

“…The appearance of organized nebulin-containing structures in cultures only two hours after the removal of fusion block correlates well with reports of rapid myosin organization following release from fusion block (Molinaro et al, 1974;Adamo et al, 1976). (Stockdale and O'Neill, 1972;Paterson and Strohman, 1972;Morris et al, 1972;Young et al, 1975;Shani et al, 1981). Shani et al (1981) One interesting observation obtained with cardiac cultures in my studies was that, in cells which appeared to be in the process of assembling myofibrils, bright fluorescence was seen inside the cells near membrane junctions between adjacent cardiac cells.…”

“…The coordinate accumulation of myofibrillar proteins (myosin heavy chains, myosin light chains, a-and ^-tropomyosin, troponin T and C) in vitro, beginning at fusion, has been demonstrated by Devlin and Emerson (1978) by two-dimensional gel electrophoresis of [ Sj-methionine labeled quail cultures. Evidence for the coordinate accumulation of embryonic myofibrillar proteins ^ vivo also has been reported (Masaki and Yoshizaki, 1972;Obinata et al, 1974 (Stockdale and O'Neill, 1972;Paterson and Strohman, 1972;Morris et al, 1972;Young et al, 1975;Shani et al, 1981). In chick primary cultures, a ten-fold increase in actin synthesis coincident with fusion has been described (Paterson et al, 1974).…”

“…The Welp chickens reach an average weight of 2.3 kg at an age of 56 to 60 days.Myogenic cell cultures derived from the slower-growing Hy-line embryos will be referred to herein as slow cultures and myogenic cultures derived from the Welp embryos will be referred to herein as fast cultures.2. Cell culture proceduresMyogenic cells were isolated from mixed leg and thigh muscles of 12 to 13 day old chick embryos by mechanical dissociation as described byYoung et al (1975). Cell suspensions were pre-plated for 40 min at 37° C to increase the percentage of myogenic cells.…”