Effect of n−3 polyunsaturated fatty acid supplementation on lipid peroxidation of rat organs

Abstract

The present study was undertaken in order to reexamine the effect of n-3 polyunsaturated fatty acid (PUFA)-rich diet supplementation on lipid peroxidation and vitamin E status of rat organs. Male Wistar rats were fed a diet containing safflower or fish oil at 50 g/kg diet and an equal amount of vitamin E at 59 mg/kg diet (1.18 g/kg oil; and 1.5 g/kg PUFA in safflower oil diet, and 4.3 g/kg PUFA in fish oil diet) for 6 wk. Fatty acid composition of total lipids of brain, liver, heart, and lung of rats fed fish oil was rich in n-3 PUFA, whereas that of each organ of rats fed safflower oil was rich in n-6 PUFA. The vitamin E levels in liver, stomach, and testis of the fish oil diet group were slightly lower than those of the safflower oil diet group, but the levels in brain, heart, lung, kidney, and spleen were not different between the two diet groups. The levels of phospholipid hydroperoxides were determined by the high-performance liquid chromatography-chemiluminescence method and the levels of thiobarbituric acid-reactive substances (TBARS) were determined at pH 3.5 in the presence of butylated hydroxytoluene with or without EDTA. Levels of phospholipid hydroperoxides and TBARS in the brain, liver, heart, lung, kidney, spleen, stomach and testis of the fish oil diet group were similar to those of the safflower oil diet group. The results indicate that high fish oil intake does not induce increased levels of phospholipid hydroperoxides and TBARS in rat organs.

Effect of Dietary Docosahexaenoic Acid Connecting Phospholipids on the Lipid Peroxidation of the Brain in Mice

“…In the study of rats fed safflower and fish oil diets for 6 wk, there were no significant differences in the thiobarbituric acid-reactive substances (TBARS), PC-OOH, or PE-OOH in the erythrocyte membranes among the groups (13). Under conditions when the rats underwent ferric nitrilotriacetic acid (Fe-NTA) oxidative stress, the increased level of TBARS in the livers was significantly higher in the fish oil diet group than in the safflower oil diet group; however, the 8-hydroxydeoxyguanosine (8-OHdG) level in the livers of the rats fed the fish oil diet group was remarkably lower than that in the livers of the safflower diet group (14).…”

Effects of cold‐pressed and vitamin E‐enriched flaxseed oils on lipid profile and antioxidant status in high‐fat fed rats

“…In addition, polyphenols also could exert cardiovascular benefits by altering the lipid profiles of plasma and liver [28]. Many studies have proved that consuming high dose of n-3 PUFA could lead to the decreased antioxidant contents and increased possibility of lipid oxidation in tissues, blood, and urine due to the increase of PUFA in membrane lipids of human and animals [29]. Our studies indicated that rats fed CFO had higher plasma T-AOC, vitamin E content, GSH, SOD, and GPX activities than those fed HRFO.…”

Effect of Supplementation of n-3 Polyunsaturated Fatty Acids on Oxidative Stress-Induced DNA Damage of Rat Hepatocytes

“…The fatty acid composition of total lipids in isolated liver of the fish oil diet group was n-3 PUFA rich and that in the safflower oil diet group was n-6 PUFA rich. 5) Hepatocytes were incubated with ADP/Fe(II) ion or hydrogen peroxide to induce oxidative stress in vitro. The degree of lipid peroxidation of oxidized hepatocytes was assessed based on the levels of the phospholipid hydroperoxides PCOOH and PEOOH and the levels of TBARS.…”