Effect of N-Acetyl-D-Glucosamine on Gene Expression in Vibrio parahaemolyticus

Thompson, Fabiano L.; Neto, Antonio A. Oliveira; Santos, Eidy de Oliveira; Izutsu, Kaori; Iida, Tetsuya

doi:10.1264/jsme2.me10152

Cited by 13 publications

(8 citation statements)

References 44 publications

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“…UDPG can be used as a precursor of polysaccharides such as exopolysaccharide (EPS), which are important to maintaining biofilms of Staphylococcus epidermidis, Vibrio parahaemolyticus, and A. baumannii [9], [21], [22]. In particular, proteome data on A. baumannii biofilm cells indicated that EPS matrix formation occurs via the Leloir pathway [23].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, N -acetyl-D-glucosamine (GlcNAc) appears to influence a variety of cellular processes, including energy metabolism, chitin utilization, competence, biofilm formation and pathogenicity in bacteria [22]. For example, a homopolymer of GlcNAc is located on the cell surfaces of Staphylococcus epidermidis and S. aureus , where it serves as an essential factor for biofilm formation [21], [29].…”

Section: Discussionmentioning

confidence: 99%

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1H NMR-Based Metabolite Profiling of Planktonic and Biofilm Cells in Acinetobacter baumannii 1656-2

et al. 2013

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Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

1H NMR-Based Metabolite Profiling of Planktonic and Biofilm Cells in Acinetobacter baumannii 1656-2

et al. 2013

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“…Recently, transcriptional evidence that the V. fischeri population in the mature squid light‐organ symbiosis is exposed to chitin as a nutrient has been supported by the discovery of host blood cells carrying particulate chitin in the tissue surrounding the symbionts (Heath‐Heckman and McFall‐Ngai, 2011). In addition, for both V. cholerae and V. parahaemolyticus , exposure to GlcNAc leads to the induction of genes associated with GlcNAc utilization (Meibom et al ., 2004; Thompson et al ., 2011). Recently, the nagA and nagB genes have been implicated in having an impact on host colonization by V. cholerae (Ghosh et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes

Miyashiro

Klein

Oehlert

et al. 2011

Molecular Microbiology

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Summary To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl-D-glucosamine-utilization genes that are co-regulated during productive symbiosis. We also demonstrate that repression by NagC is relieved in the presence of N-acetyl-D-glucosamine-6-phosphate, the intracellular form of N-acetyl-D-glucosamine. We find that gene repression by NagC is critical for efficient colonization of E. scolopes. Further, our study shows that NagC regulates genes that affect the normal dynamics of host colonization.

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“…The genes encoding these enzymes are under the control of the same system, known as the nag regulon. Some studies have examined this gene cluster (21,22), and there are reports of chitin-derived sugars inducing the expression of these genes (13,23). nagE, nagA, nagC, and nagB correspond to the N-acetylglucosamine-specific IIABC component (VP0831), N-acetylglucosamine-6-phosphate deacetylase (VP0829), the N-acetylglucosamine repressor (VP0828), and glucosamine-6-phosphate deaminase (VPA0038), respectively.…”

Section: Discussionmentioning

confidence: 99%

Chitin Heterodisaccharide, Released from Chitin by Chitinase and Chitin Oligosaccharide Deacetylase, Enhances the Chitin-Metabolizing Ability of Vibrio parahaemolyticus

et al. 2019

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Vibrio parahaemolyticus RIMD2210633 secretes both chitinase and chitin oligosaccharide deacetylase and produces β-N-acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) from chitin. Previously, we reported that GlcNAc-GlcN induces chitinase production by several strains of Vibrio harboring chitin oligosaccharide deacetylase genes (T. Hirano, K. Kadokura, T. Ikegami, Y. Shigeta, et al., Glycobiology 19:1046–1053, 2009). The metabolism of chitin by Vibrio was speculated on the basis of the findings of previous studies, and the role of chitin oligosaccharide produced from chitin has been well studied. However, the role of GlcNAc-GlcN in the Vibrio chitin degradation system, with the exception of the above-mentioned function as an inducer of chitinase production, remains unclear. N,N′-Diacetylchitobiose, a homodisaccharide produced from chitin, is known to induce the expression of genes encoding several proteins involved in chitin metabolism in Vibrio strains (K. L. Meibom, X. B. Li, A. Nielsen, C. Wu, et al., Proc Natl Acad Sci U S A 101:2524–2529, 2004). We therefore hypothesized that GlcNAc-GlcN also affects the expression of enzymes involved in chitin metabolism in the same manner. In this study, we examined the induction of protein expression by several sugars released from chitin using peptide mass fingerprinting and confirmed the expression of genes encoding enzymes involved in chitin metabolism using real-time quantitative PCR analysis. We then confirmed that GlcNAc-GlcN induces the expression of genes encoding many soluble enzymes involved in chitin degradation in Vibrio parahaemolyticus. Here, we demonstrate that GlcNAc-GlcN enhances the chitin-metabolizing ability of V. parahaemolyticus. IMPORTANCE We demonstrate that β-N-acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) enhances the chitin-metabolizing ability of V. parahaemolyticus. Members of the genus Vibrio are chitin-degrading bacteria, and some species of this genus are associated with diseases affecting fish and animals, including humans (F. L. Thompson, T. Iida, and J. Swings, Microbiol Mol Biol Rev 68:403–431, 2004; M. Y. Ina-Salwany, N. Al-Saari, A. Mohamad, F.-A. Mursidi, et al., J Aquat Anim Health 31:3–22, 2019). Studies on Vibrio are considered important, as they may facilitate the development of solutions related to health, food, and aquaculture problems attributed to this genus. This report enhances the current understanding of chitin degradation by Vibrio bacteria.

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Effect of N-Acetyl-D-Glucosamine on Gene Expression in Vibrio parahaemolyticus

Cited by 13 publications

References 44 publications

1H NMR-Based Metabolite Profiling of Planktonic and Biofilm Cells in Acinetobacter baumannii 1656-2

1H NMR-Based Metabolite Profiling of Planktonic and Biofilm Cells in Acinetobacter baumannii 1656-2

The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes

Chitin Heterodisaccharide, Released from Chitin by Chitinase and Chitin Oligosaccharide Deacetylase, Enhances the Chitin-Metabolizing Ability of Vibrio parahaemolyticus

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