Effect of N-acetyl-L-cysteine Supplementation in Semen Extenders on Semen Quality and Reactive Oxygen Species of Chilled Canine Spermatozoa

Abstract

The objective of this study was to evaluate the quality of chilled dog semen processed with extenders containing various concentrations of N-acetyl-L-cysteine (NAC). Ejaculates from five dogs were collected, pooled and evaluated for concentration, motility, rapid steady forward movement (RSF-movement), viability, acrosomal integrity and by the hypo-osmotic swelling test (HOST). In addition, superoxide anion (O(2)(-*)) production, hydroxyl radicals (OH(*)) and total reactive oxygen species (tROS) were determined. The pool was divided into five aliquots, which were diluted to a final concentration of 66.66 x 10(6) spermatozoa/ml with Tris-glucose-egg yolk extender containing one of the following concentrations of NAC (0, 0.5, 1, 2.5 or 5 mm). The semen aliquots were chilled and preserved at 4 degrees C. Semen quality was evaluated after rewarming at 72 h. Sperm motility was significantly higher with the 0.5 mm concentration compared with the control group (p = 0.001). Rapid steady forward movement was higher with the 0.5 and 1 mm concentrations compared with the control and 5 mm group (p < 0.001). Viability and HOST percentages were not significantly altered. Compared with the control, the 5 mm concentration showed significantly reduced percentages of spermatozoa with normal acrosomes (p = 0.049). None of the ROS values at 72 h were significantly affected by the presence of NAC in semen extenders, although all NAC concentrations showed lower O(2)(-*) and OH(*) values compared with the control. Only the concentrations of 1 and 5 mm inhibited the significant increase of tROS values after 72 h, compared with the fresh semen value. In conclusion, NAC supplementation of semen extenders is beneficial to semen motility of canine spermatozoa during chilling with the 0.5 mm concentration being the most effective, although no significant ROS inhibition was observed at 72 h.

Potential Ameliorative Role of N-Acetylcysteine Against Testicular Dysfunction Induced by Titanium Dioxide in Male Albino Rats

“…The present study demonstrated that aromatase mRNA levels decreased in TiO2 administered rats and NAC normalized aromatase expression in a way to control estradiol biosynthesis (Shi et al, 2007). It has been shown that NAC decrease diazinon induced oxidative stress in rat testes (Oksay et al, 2013) and improved semen quality and canine fertility (Michael et al 2010).…”

Efeito da adição de glutationa peroxidase e cisteína ao diluidor de congelação do sêmen equino

“…O efeito positivo da adição de 1mM de cisteína na porcentagem de espermatozoides com acrossomas íntegros (IAc), no tempo T60 pós-descongelação, em relação aos grupos GPx 1 e 5U e cisteína 0,5mM, pode estar relacionado à inibição da fosforilação da tirosina que atua na capacitação espermática, através do bloqueio dos canais de íons na membrana plasmática (Varner, 2007), melhorando a integridade da membrana espermática pós-descongelação (Pagl et al, 2006), sem eliminar a presença de ROS (Michael et al, 2008). Esperava-se, também, que esses antioxidantes promovessem maior porcentagem de gametas com aPMM, devido à maior proteção às mitocôndrias (Sariozkan et al, 2009), resultando em maior motilidade (MT e MP).…”

N-Asetilsistein (NAC)’in Dondurulmuş-Çözdürülmüş Tuj Koçu Spermasının Kalitesi Üzerine Etkisi

“…If these non-enzymatic antioxidants are mainly oxidized and not regenerated, this could partly explain why skim milk and egg yolk is not very efficient against oxidative stress and why addition of NAC may have been beneficial to frozen-thawed sperm motility and other parameters in skim milk based egg yolk extender. NAC was added to Tris-glucose-egg yolk extender with high and low doses (0.5 or 1.5 mM) [21][22][23] . When different doses of NAC were supplemented to Tris egg yolk based extender [21][22][23] or milk based extender [10] , beneficial effects on sperm motility were determined.…”

“…NAC was added to Tris-glucose-egg yolk extender with high and low doses (0.5 or 1.5 mM) [21][22][23] . When different doses of NAC were supplemented to Tris egg yolk based extender [21][22][23] or milk based extender [10] , beneficial effects on sperm motility were determined. Although Michael et al [21,22] determined that supplementation of 1.5 mM NAC to Tris based extender improved sperm motility of frozenthawed and chilled canine semen, we qualified that higher doses of NAC (i.e.…”