Effect of N-Acetylcysteine and Fructose-1,6-Bisphosphate in the Treatment of Experimental Sepsis

Abstract

Sepsis is a syndrome caused by uncontrolled systemic inflammatory response of the individual, which represents a serious epidemiological problem worldwide. The aim of this study was to investigate the effect of N-acetylcysteine (NAC) and fructose-1,6-bisphosphate (FBP) in the treatment of experimental sepsis. We used rats that were divided into five experimental groups: normal control (not induced), septic control (induced using a capsule with non sterile fecal content and Escherichia coli), treated with FBP (500 mg/kg i.p.), treated with NAC (150 mg/kg i.p.), and treated with the combination of FBP with NAC. In the group treated with NAC, 16.68% of the mice survived, the FBP reduced the mortality of mice during the acute stage of the disease and increased the animals' survival time in 33.34%, and the combination of drugs had no effect. Our results show that NAC prevented the mortality of animals after septic induction. These data confirm the validity of the use of NAC in the treatment of sepsis. Our data also show that the synergistic action with FBP does not improve the picture.

N-acetylcysteine and fructose-1,6-bisphosphate: immunomodulatory effects on mononuclear cell culture

“…Previous studies in vivo of our group showed a reduction in mortality of animals subjected to experimental sepsis when treated with FBP (24) and NAC (21) , confirming an important role of both drugs in the treatment of sepsis. On the other hand, when rats were treated with combination of NAC and FBP, there was not improvement of the picture (21) .…”

“…On the other hand, when rats were treated with combination of NAC and FBP, there was not improvement of the picture (21) . Extensive evidence in the literature demonstrates a correlation between the increased proinflammatory cytokines production and the sepsis mortality rate, both in humans and experimental models, being the IL-1 and IL-6 cytokines suggested to play a key role.…”

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

“…Animal experiments have confirmed that N-acetylcysteine can reduce oxidative stress damage and the release of inflammatory mediators, which is beneficial to sepsis recovery [ 22 , 23 ]. Other experimental studies have shown that acetylcysteine can reduce organ injury [ 24 ] and improve survival [ 25 ]. The role of cysteine in the treatment of sepsis has also been confirmed in clinical trials [ 26 , 27 ].…”

Fructose-1,6-Bisphosphate Reduces the Mortality in Candida albicans Bloodstream Infection and Prevents the Septic-Induced Platelet Decrease

“…This seems to be critical for the resolution of the bloodstream infection, since the lowest counts of platelets correlate with higher mortality rates (that occur within the first 48 h after induction of the infectious process). The role of FBP on platelets has been recently reported, being an important platelet aggregation inhibitor [22]. The inhibition of the platelet aggregation can be very important since this can improve the tissue perfusion that is harmed in the septic shock for the formation of clots [23].…”