1989
DOI: 10.1007/bf00609183
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Effect of N-acetylcysteine on plasma cysteine and glutathione following paracetamol administration

Abstract: The effect of oral N-acetyl-L-cysteine (NAC) on plasma sulphhydryls has been studied in healthy volunteers. Following NAC 30 mg.kg-1, total NAC in plasma (i.e. free NAC and NAC as disulphides) reached a median peak concentration of 67 nmol.ml-1 within 45 to 60 min, and disappeared with an apparent half-life of 1.3 h. Only a fraction of total NAC (AUC 163 nmol.ml-1.h) was in the form of free NAC (AUC 12 nmol.ml-1.h, peak concentration 9 nmol.ml-1). Free cysteine was markedly increased (peak increment 49 nmol.ml… Show more

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Cited by 220 publications
(130 citation statements)
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“…Moreover, the increased intracellular peroxides were completely suppressed by preincubation with 10 mM N-acetyl-L-cysteine (Fig. 7C), which is capable of causing an increase in intracellular GSH levels thus resulting in the scavenging of oxidants both directly and indirectly (37)(38)(39). After treatments with aminoguanidine or NAC and 3-DG, similar results were observed in intracellular peroxide production (data not shown).…”
Section: Effects Of Cycloheximide Actinomycin D and Aminoguanidine supporting
confidence: 72%
“…Moreover, the increased intracellular peroxides were completely suppressed by preincubation with 10 mM N-acetyl-L-cysteine (Fig. 7C), which is capable of causing an increase in intracellular GSH levels thus resulting in the scavenging of oxidants both directly and indirectly (37)(38)(39). After treatments with aminoguanidine or NAC and 3-DG, similar results were observed in intracellular peroxide production (data not shown).…”
Section: Effects Of Cycloheximide Actinomycin D and Aminoguanidine supporting
confidence: 72%
“…Ingestion of NAC in quantities that can double the plasma concentrations of cysteine by a reduction of cystine (64,65) appears to be well tolerated. The model predicted that a 50% increase in the extracellular cysteine concentration could substantially mitigate the effect of the increased rate of oxidation (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…Indeed, several reports have linked expression of H-RasV12 with the induction of DNA damage, chromosomal instability, and aneuploidy (Denko et al 1994;Lee et al 1999). The chromosomal instability caused by HRasV12 could be rescued by treating the cells with Nacetyl-cysteine (NAC; Lee et al 1999), a precursor of reduced glutathione and a scavenger of reactive oxygen species (ROS; Aruoma et al 1989;Burgunder et al 1989). Consistent with these reports, we confirmed that H-RasV12 induced ROS in the SF-MEFs as detected by staining with the redox-sensitive fluorochrome, dichlorofluorescein diacetate (DCFDA), and NAC was effective in blocking the Ras-induced ROS production (Supplementary Fig.…”
Section: Oncogenic Ras Evokes a P53-dependent Apoptotic Response In Smentioning
confidence: 99%