Effect of N and P fertilisation and aeration on biodegradation of crude oil in aged hydrocarbon contaminated soils

Syafruddin, S; Wieshammer, Gerlinde; Puschenreiter, Markus; Langer, Ingrid; Wieshammer-Zivkovic, Michael; Wenzel, Walter W.

doi:10.17221/146/2009-pse

Cited by 11 publications

(7 citation statements)

References 15 publications

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“…Mineralisation of dodecane by strain Q15 was assessed through 96-well plates without any shaking condition whereas strain ADL36 was cultured as described in Materials and methods. A shaking condition greatly affected the aeration of oxygen in cultured media as oxygen plays a significant role in determining the rate of hydrocarbon degradation [ 58 – 60 ]. With regards to the genus Pseudomonas , two psychrotrophic Pseudomonas strains able to mineralise dodecane was reported [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

et al. 2018

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BackgroundBiodegradation of hydrocarbons in Antarctic soil has been reported to be achieved through the utilisation of indigenous cold-adapted microorganisms. Although numerous bacteria isolated from hydrocarbon-contaminated sites in Antarctica were able to demonstrate promising outcomes in utilising hydrocarbon components as their energy source, reports on the utilisation of hydrocarbons by strains isolated from pristine Antarctic soil are scarce. In the present work, two psychrotolerant strains isolated from Antarctic pristine soil with the competency to utilise diesel fuel as the sole carbon source were identified and optimised through conventional and response surface method.ResultsTwo potent hydrocarbon-degraders (ADL15 and ADL36) were identified via partial 16S rRNA gene sequence analysis, and revealed to be closely related to the genus Pseudomonas and Rhodococcus sp., respectively. Factors affecting diesel degradation such as temperature, hydrocarbon concentration, pH and salt tolerance were studied. Although strain ADL36 was able to withstand a higher concentration of diesel than strain ADL15, both strains showed similar optimal condition for the cell’s growth at pH 7.0 and 1.0% (w/v) NaCl at the conventional ‘one-factor-at-a-time’ level. Both strains were observed to be psychrotrophs with optimal temperatures of 20 °C. Qualitative and quantitative analysis were performed with a gas chromatograph equipped with a flame ionisation detector to measure the reduction of n-alkane components in diesel. In the pre-screening medium, strain ADL36 showed 83.75% of n-dodecane mineralisation while the reduction of n-dodecane by strain ADL15 was merely at 22.39%. The optimised condition for n-dodecane mineralisation predicted through response surface methodology enhanced the reduction of n-dodecane to 99.89 and 38.32% for strain ADL36 and strain ADL15, respectively.ConclusionsStrain ADL36 proves to be a better candidate for bioaugmentation operations on sites contaminated with aliphatic hydrocarbons especially in the Antarctic and other cold regions. The results obtained throughout strongly supports the use of RSM for medium optimisation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

et al. 2018

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“…However, the biodegradation efficiency of BF40 inoculation was significant lower than that of surfactant addition in soils with different salinity. The possible explanation was that microbial growing conditions, such as soil constitution (Syafruddin et al 2010, Roldán-Carrillo et al 2011) and salinity ( Figure 2B, Figure 4), could significantly influence the microbial surface activity, although BF40 strain possessed salt-tolerant ability.…”

Section: Resultsmentioning

confidence: 99%

Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

Wu¹,

Xie²,

Yi³

et al. 2012

PLANT SOIL ENVIRON

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An ideal strain for crude oil degradation in saline soils would be one with high salt-tolerance. A novel bacterial strain, Serratia sp. BF40, was isolated from crude oil contaminated saline soils. Its salt-tolerance, surface activity and ability to degrade crude oil in saline soils were evaluated. It can grow in liquid culture with NaCl concentration less than 6.0%. Its surface activity characterized as an efficient surface tension reduction, was significantly affected by salinity above 2.0%. BF40 inoculation could decrease surface tension of soil solutions and facilitate crude oil removal in soils with 0.22-1.20% salinity, but the efficiency was both significantly lower than its biosurfactant addition. The BF40 strain has a high potential for biodegradation of crude oil contaminated saline soils in view of its high surface activity and salt-tolerance, which is the first report of biosurfactant producing by the genus Serratia for petroleum degrading. We suggest that biosurfactant addition is an efficient strategy. Simultaneously, the growing status of the strain and how to boost its surface activity in saline soils should deserve further studies in order to achieve a continuous biosurfactant supply.

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“…One important factor, which influences the degradability of compounds, is pH (Cirelli et al, 2008;Anwar et al, 2009;Syafruddin et al, 2010). The surfactant degrading ability was studied at three different pH conditions: acidic soil I (4.40), acidic soil II (4.90), and basic soil III (7.69).…”

Section: Factors Effecting the Degradation Of Surfactants In Nis Treamentioning

confidence: 99%

Effect of Nitrification Inhibitors on Degradation of Surfactants in Soils

LuoSi

HeChang

ZhouXihong

et al. 2015

Environmental Engineering Science

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This study investigated the effects of nitrification inhibitors (NIs) on the degradation of two selected surfactants, an anionic surfactant, linear alkylbenzene sulfonates (LAS), and a nonionic surfactant, Brij 30, in soils. Soils were treated with different NIs, mixed with LAS or Brij 30 and incubated. Results revealed that the addition of NIs, such as thiourea (TU), dicyandiamide (DCD), and hydroquinone (HQ), could significantly inhibit the degradation of surfactants and expand their half-lives in soil. When the initial concentration of NIs was 2.5 mmol/kg soil, their inhibition effects followed the order of HQ > TU > DCD for LAS and HQ > DCD & TU for Brij 30. In addition, significant differences between nonsterile and sterile treatments demonstrated that microorganism activity degradation plays a major role in the degradation of surfactants. Degradation efficiency of surfactants in soils varied with initial NIs concentration and environmental factors such as culture temperature and soil moisture. Inhibition effects of NIs on the degradation of LAS and Brij 30 were reduced without exception in alkaline soil. This study could provide the scientific basis for the reasonable evaluation and management of these agrochemicals.

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Effect of N and P fertilisation and aeration on biodegradation of crude oil in aged hydrocarbon contaminated soils

Cited by 11 publications

References 15 publications

Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

Effect of Nitrification Inhibitors on Degradation of Surfactants in Soils

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