Effect of N-carbamoylglutamate supplementation on the growth performance, antioxidant status and immune response of mirror carp (Cyprinus carpio) fed an arginine-deficient diet

Wang, Liansheng; Li, Jinnan; Wang, Chang‐An; Zhao, Zhigang; Luo, Liang; Du, Xiang; Xu, Qiyou

doi:10.1016/j.fsi.2018.10.001

Cited by 27 publications

(24 citation statements)

References 78 publications

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“…Citrulline synthesis from glutamine as well as the sparing effect of glutamate on dietary arginine requirement has been observed in I. punctatus (Buentello & Gatlin 2000), in which supplementation of glutamine (2.08% of dietary protein) to an arginine‐deficient diet (2% of dietary protein) significantly increased feed efficiency and plasma citrulline and arginine levels of the fish. Similar results were observed in C. carpio (arginine level of 3.8% and glutamine level 0.37–0.62% of dietary protein) and O. niloticus (arginine level of 3.8% and glutamine level 0.37–0.62% of dietary protein) (Li et al, 2015; Wang et al, 2019). These studies suggest that glutamine positive effects on fish growth performance are observed when fish are fed arginine‐deficient diets.…”

Section: Arginine Interactions With Lysine and Glutamatesupporting

confidence: 73%

“…Li et al (2015) observed that dietary N‐carbamoylglutamate (NCG) supplementation significantly increased Oreochromis niloticus growth rate and blood arginine level. Growth rate and blood arginine levels of Cyprinus carpio fed with NCG‐supplemented diet were comparable to fish fed with the arginine‐supplemented diet (Wang et al, 2019). Contrasting those previous studies, Pereira et al (2017) did not observe any effects of dietary glutamine supplementation on plasma free arginine levels in O. niloticus , but a depression in the fish growth rate and protein retention.…”

Section: Biosynthesis Of Arginine In Urea Cycle and Intestinal–renal mentioning

confidence: 67%

“…Interestingly, anti‐inflammatory cytokines gene expression increased only in the fish submitted to the higher levels of dietary arginine administration, a fact that could be related to a feedback mechanism to counteract negative effects of acute inflammation (Chen et al, 2015). In contrast, a recent study on C. carpio (mirror phenotype) suggested that dietary arginine supplementation down‐regulated pro‐inflammatory cytokine genes (TNF‐α, IL‐1β and IL‐8) and up‐regulated anti‐inflammatory cytokine genes (TGF‐β and IL‐10) in the intestine (Wang et al, 2019). Other studies suggested that arginine seems to have anti‐inflammatory properties in order to suppress negative effects of inflammation in tissues from different species exposed to LPS stimulation (Holen et al, 2014; Jiang et al, 2015; Martins et al, 2019).…”

Section: Inflammationmentioning

confidence: 93%

“…In contrast, dietary arginine up to an optimum level (based on fish growth response) had no significant effects on serum malondialdehyde levels and antioxidant enzyme activities in Pelteobagrus fulvidraco , while an increase in dietary arginine levels beyond the fish requirement led to significant decrease in the fish growth response, serum malondialdehyde levels and antioxidant enzyme activities (Zhou et al, 2015). Wang et al (2019) found a decreased growth performance and antioxidant enzyme activity and increased malondialdehyde levels in the gut of C. carpio fed an arginine‐deficient diet. In Ctenopharyngodon idella , graded levels of dietary arginine led to a broken‐line growth response but polynomial responses of different antioxidant markers including malondialdehyde and protein carbonyl contents, antioxidant enzyme activities and gene expressions (Wang et al, 2015).…”

Section: Antioxidant Effects Of Argininementioning

confidence: 99%

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Roles of arginine in fish nutrition and health: insights for future researches

Hoseini

Khan

Yousefi

et al. 2020

Reviews in Aquaculture

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Amino acids are important molecules in fish nutrition with critical roles in protein synthesis, immune function and health. Among the essential amino acids, arginine is one of the functional amino acids in animals. Arginine requirement in fish ranges between 3.0 and 8.1% of dietary protein. The amino acid involves in ureagenesis, immune function, antioxidant defence, somatotropic axis, stress responses and ammonia detoxification in fish. To have an extended insight about the current knowledge of arginine effects in fish, this article reviewed available data on arginine effects in different fish species. It covers variations in arginine requirements of different fish species, interaction of arginine with lysine and glutamine, modulation of ureagenesis and ammonia detoxification, modulation of insulin and growth hormone responses, cortisol response, modulation of antioxidant system, immune responses and disease resistance. This review indicates that rearing conditions and dietary ingredients may affect fish arginine requirement. In addition, there is a need for further data about the antioxidant effects of arginine under oxidative condition, its immunostimulant effects under pathogenic challenge, mechanisms of ammonia detoxification and cortisol response by arginine in different fish species.

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Section: Arginine Interactions With Lysine and Glutamatesupporting

confidence: 73%

Section: Biosynthesis Of Arginine In Urea Cycle and Intestinal–renal mentioning

confidence: 67%

Section: Inflammationmentioning

confidence: 93%

Section: Antioxidant Effects Of Argininementioning

confidence: 99%

See 2 more Smart Citations

Roles of arginine in fish nutrition and health: insights for future researches

Hoseini

Khan

Yousefi

et al. 2020

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…The dietary administration of NCG has been shown to alleviate intestinal inflammation in the fish fed Arg-deficient diet [66]. In both studies, L-Arg and NCG modulated the anti-inflammatory response via regulating the mRNA expression of pro-and anti-inflammatory cytokines [65,66].…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation of L-Arginine and N-Carbamylglutamate Attenuated the Hepatic Inflammatory Response and Apoptosis in Suckling Lambs with Intrauterine Growth Retardation

Zhang

Fan

Elsabagh

et al. 2020

Mediators of Inflammation

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L-arginine (Arg) is a semiessential amino acid with several physiological functions. N-Carbamylglutamate (NCG) can promote the synthesis of endogenous Arg in mammals. However, the roles of Arg or NCG on hepatic inflammation and apoptosis in suckling lambs suffering from intrauterine growth restriction (IUGR) are still unclear. The current work is aimed at examining the effects of dietary Arg and NCG on inflammatory and hepatocyte apoptosis in IUGR suckling lambs. On day 7 after birth, 48 newborn Hu lambs were selected from a cohort of 432 twin lambs. Normal-birthweight and IUGR Hu lambs were allocated randomly (n = 12/group) to control (CON), IUGR, IUGR+1% Arg, or IUGR+0.1% NCG groups. Lambs were fed for 21 days from 7 to 28 days old. Compared with CON lambs, relative protein 53 (P53), apoptosis antigen 1 (Fas), Bcl-2-associated X protein (Bax), caspase-3, cytochrome C, tumor necrosis factor alpha (TNF-α), nuclear factor kappa-B (NF-κB) p65, and NF-κB pp65 protein levels were higher (P < 0:05) in liver from IUGR lambs, whereas those in liver from IUGR lambs under Arg or NCG treatment were lower than those in IUGR lambs. These findings indicated that supplementing Arg or NCG reduced the contents of proinflammatory cytokines at the same time when the apoptosis-related pathway was being suppressed, thus suppressing the IUGR-induced apoptosis of hepatic cells.

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Effects of dietary N‐carbamylglutamate supplementation on the modulation of microbiota and Th17/Treg balance‐related immune signaling after lipopolysaccharide challenge

Liu,

Zhang,

Liu

et al. 2023

J Sci Food Agric

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BackgroundThis study aimed to evaluate the effect of N‐carbamylglutamate (NCG) on the piglets’ growth performance and immune response, and unravel their mechanisms. In a 2 × 2 factorial design including diet (with or without NCG) and immunological challenge (saline or lipopolysaccharide (LPS)), 24 piglets were randomly distributed to four groups. After being fed a basic diet or a NCG‐supplemented diet for 21 days, piglets were administered LPS or saline intraperitoneally.ResultsThe results showed that NCG increased average daily gain and average daily feed intake, and feed conversion ratio of piglets, and alleviated the adverse effects of LPS stimulation about intestinal morphology. At the phylum level, NCG reversed the changes of the increase in Firmicutes and decrease in Actinomycete abundance caused by LPS stimulation. At the genus level, NCG increased the abundances of Lactobacillus, Blautia, norank_Butyricicoccaceae, Subdoligranulum, and Ruminococcus_gauvreauii_group, while LPS decreased the abundances of Escherichia‐Shigella and Ruminococcus_gauvreauii_group. The content of short‐chain fatty acids was raised by NCG, while LPS reduced its content. Additionally, NCG significantly inhibited the LPS‐induced increase in the relative expression of signal transducer and activator of transcription (STAT) 3, RAR related orphan receptor c, and pro‐inflammatory cytokines, and the decrease in the relative expression of STAT5, forkhead box P3, IL‐10 and transforming growth factor‐beta 1 mRNAs. A significant correlation was found between intestinal microbiota and inflammatory cytokines and short‐chain fatty acids.ConclusionCollectively, NCG can improve piglets’ growth performance. NCG can also attenuate LPS‐induced intestinal inflammation by modulating microbiota and Th17/Treg balance‐related immune signaling pathways.This article is protected by copyright. All rights reserved.

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Effect of N-carbamoylglutamate supplementation on the growth performance, antioxidant status and immune response of mirror carp (Cyprinus carpio) fed an arginine-deficient diet

Cited by 27 publications

References 78 publications

Roles of arginine in fish nutrition and health: insights for future researches

Roles of arginine in fish nutrition and health: insights for future researches

Dietary Supplementation of L-Arginine and N-Carbamylglutamate Attenuated the Hepatic Inflammatory Response and Apoptosis in Suckling Lambs with Intrauterine Growth Retardation

Effects of dietary N‐carbamylglutamate supplementation on the modulation of microbiota and Th17/Treg balance‐related immune signaling after lipopolysaccharide challenge

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