Effect of N&lt;sup&gt;6&lt;/sup&gt;-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

Stoyanova-Koleva, D.; Stefanova, M.; Zhiponova, Miroslava; Kapchina-Toteva, Veneta

doi:10.1007/s10535-012-0060-z

Cited by 9 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, the relationship between starch accumulation in tissues and regeneration potential has been reported for many species (Chen and Ziv, 2005;Fortes and Pais, 2000). Studies have indicated that higher starch levels are correlated with higher regeneration potentials (Chen and Ziv, 2005;Stoyanova-Koleva et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation Shortens the Time to Blooming of Begonia montaniformis × Begonia ningmingensis var. bella F1 Progeny

Lai¹,

Lin²,

Chen³

et al. 2018

horts

View full text Add to dashboard Cite

Additional index words. plant growth regulator, light quality, shoot organogenesis, Begonia Abstract. Begonia montaniformis 3 Begonia ningmingensis var. bella hybrids have high ornamental potential. Hence, the aim of this study was to determine the optimal conditions for the micropropagation of a Begonia montaniformis 3 Begonia ningmingensis var. bella F 1 progeny by using various concentrations of plant growth regulators (PGRs) and varying light spectra in half-strength Murashige and Skoog (1/2 MS) medium. The results showed that the explant regeneration was optimal when the lamina was incubated in a medium supplemented with 2.0 mM N 6 -benzylaminopurine and 0.8 mM a-naphthaleneacetic acid (NAA). Under such conditions, 98% of the explants regenerated adventitious shoots after 8 weeks, and 41 buds were produced per explant on average. The mean shoot length was 9.6 mm, and on average, 4.5 shoots per explant were more than 2 mm long. Subsequently, the induced adventitious shoots were transferred into rooting medium consisting of 1/2 MS and various NAA concentrations. After 4 weeks, the shoots subcultured in this medium showed ' '93% root induction and an average of 3.5 adventitious roots per explant. Furthermore, the applied light spectrum significantly influenced shoot regeneration, and optimal results were achieved under an equal distribution of blue, red, and infrared light. The histological sections of shoots regenerated from direct organogenesis were observed through scanning electron microscopy (SEM). Afterward, the rooting adventitious shoots were subcultured in PGR-free medium for 8 weeks. The seedlings were successfully acclimated 4 weeks after being transferred to soil and bloomed after 11 months in a greenhouse. Thus, the PGR composition in micropropagation efficiently shortened the time to blooming from 25 to 16 months.

show abstract

Section: Discussionmentioning

confidence: 99%

Micropropagation Shortens the Time to Blooming of Begonia montaniformis × Begonia ningmingensis var. bella F1 Progeny

Lai¹,

Lin²,

Chen³

et al. 2018

horts

View full text Add to dashboard Cite

show abstract

“…According to some studies, a common reaction to low temperatures is a collapse of the photosynthetic apparatus (Danova et al 2009, Ganeva et al 2009. Studies of the leaf structure of in vitro cultured plants revealed that the examination on subcellular level is very important for evaluation of plant regeneration potential (Stefanova 2011, Stoyanova-Koleva et al 2012.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Chloroplast ultrastructure of Hypericum perforatum plants regenerated in vitro after cryopreservation

Stoyanova-Koleva¹,

Stefanova²,

Čellárová³

et al. 2013

Biol. plant.

Self Cite

View full text Add to dashboard Cite

The ultrastructure of leaf mesophyll cells of in vitro cultured Hypericum perforatum L. plants regenerated after cryopreservation was studied. Electron microscopy analysis revealed that the chloroplasts in plants pretreated with abscisic acid and regenerated after cryopreservation were round, with increased amount of starch, rather small volume of the thylakoid system, and destroyed envelope. Plants pretreated with 0.3 M mannitol and cooled at rates of 0.1 or 0.3 °C min -1 possessed chloroplasts with high starch content that resulted in a reduction of a membrane system. However, the pretreatment with 0.3 M mannitol and cooling at a rate of 0.2 °C min -1 was the best as chloroplast ultrastructure resembled the controls regenerated without cryopreservation.

show abstract

Enhanced Surface Disinfection and Subsequent Growth in Plant Micropropagation by Copper Nanoparticles

Tung,

Bao,

Luan

et al. 2024

Metal Nanoparticles in Plant Cell, Tissue and Organ Culture

View full text Add to dashboard Cite

Effect of N<sup>6</sup>-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

Cited by 9 publications

References 28 publications

Micropropagation Shortens the Time to Blooming of Begonia montaniformis × Begonia ningmingensis var. bella F1 Progeny

Micropropagation Shortens the Time to Blooming of Begonia montaniformis × Begonia ningmingensis var. bella F1 Progeny

Chloroplast ultrastructure of Hypericum perforatum plants regenerated in vitro after cryopreservation

Enhanced Surface Disinfection and Subsequent Growth in Plant Micropropagation by Copper Nanoparticles

Contact Info

Product

Resources

About