The effects of temperature and water potential on nitrification were investigated in two Iowa soils treated with Stay-N 2000. The soils were incubated at 10, 20, and 30 uC after soil water potentials of 21, 210, or 260 kPa were applied to each soil. A first-order equation was used to calculate the maximum nitrification rate (K max ), duration of lag period (t9), period of maximum nitrification (Dt), and termination period of nitrification (t s ). The highest K max were 18 and 24 mg kg 21 d 21 nitrate (NO 3 2 )-nitrogen (N), respectively, at 30 uC and 210 kPa in both the Nicollet (fine-loamy, mixed, superactive, mesic Aquic Hapludoll) and Canisteo (fine-loamy, mixed, superactive, calcareous, mesic Typic Endoaquoll) soils and reduced to 4 and 16 mg kg 21 d 21 NO 3 2 -N when Stay-N 2000 was added. The extension of t9 due to the addition of Stay-N 2000 was as high as 7 d in the Nicollet soil at 10 uC and 21 kPa and as little as 2 d in the Canisteo soil at 20 uC and 210 kPa.