Effect of nanoemulsion particle size on the bioavailability and bioactivity of perilla oil in rats

Abstract

The aim of this study was to investigate the bioavailability and bioactivity of perilla (Perilla frutescens) oil nanoemulsions prepared at different homogenization pressures by measuring the weight, fatty acid profile, and antioxidant and anti‐inflammatory properties in rats. The high‐pressure homogenization significantly reduced the particle size of perilla oil nanoemulsions and enhanced their stability, and the minimum particle size was 293.87 ± 6.55 nm at 120 MPa. There was an increase in the weight and fatty acid levels in the plasma and liver of test group rats. The highest glutathione (GSH) and the lowest malondialdehyde (MDA) levels of 18.76 ± 10.51 mg GSH/g prot and 20.27 ± 2.46 nmol/mg prot, respectively, were recorded in rats administrated perilla oil nanoemulsions prepared at 120 MPa. However, there was no significant difference in superoxide dismutase activity (SOD) between the groups. The interferon‐gamma (IL‐γ), interleukin‐1 beta (IL‐1β), IL‐6 (interleukin‐6), and IL‐8 (interleukin‐8) levels in the test groups were lower than those in the blank and control groups at 8 hr after lipopolysaccharide injection. The IL‐1β, IL‐6, and IL‐8 levels were 49.52 ± 14.06, 90.13 ± 6.04, and 419.71 ± 32.03 ng/L, respectively, in rats treated with perilla oil nanoemulsions prepared at 120 MPa. Both perilla oil and its nanoemulsions decreased estradiol levels and damaged the ovaries. Overall, our findings show that the test nanoemulsions enhanced the bioavailability of perilla oil, which resulted in enhanced antioxidant and anti‐inflammatory responses; thus, we provide a new approach to deliver perilla oil.Practical ApplicationNanoemulsions can be used to deliver drugs and bioactive compounds, and perilla oil nanoemulsions can be used in healthcare products and beverage industries.

Development, Characterization, and Immunomodulatory Evaluation of Carvacrol-loaded Nanoemulsion

“…Nanoemulsions are colloidal dispersions of two immiscible liquids stabilized by surfactants with small droplet sizes [ 16 , 17 ]. The advantages of this system include the enhancement of the solubility and bioavailability of lipophilic molecules (e.g., CV), controlled drug release, and protection from enzymatic degradation [ 18 , 19 ]. Nanoemulsions can be produced using different methodologies, including high-energy methods such as high-pressure homogenization, ultrasonication, and microfluidization [ 20 , 21 , 22 , 23 ].…”

Bioavailability and biotransformation of linolenic acid from basil seed oil as a novel source of omega-3 fatty acids tested on a rat experimental model

“…Furthermore, the administration of 5 g kg −1 perilla oil for 21 days increased ALA and EPA levels in the liver. 64 In contrast, Rincón-Cervera et al 63 reported an increase in DHA levels after supplementing with rosa mosqueta, chia, and sacha inchi oils. This difference could be attributed to the higher ALA content in BSO or the different LA : ALA ratios.…”

Sanse Powder Essential Oil Nanoemulsion Negatively Regulates TRPA1 by AMPK/mTOR Signaling in Synovitis: Knee Osteoarthritis Rat Model and Fibroblast-Like Synoviocyte Isolates

“…SP-NEs were prepared employing the method of homogenization [ 21 , 24 ] using the JN-02HC high-pressure homogenizer (Juneng Biotechnology Co., Ltd., Guangzhou, China). We started with the selection of components for phase A and phase B, and the chosen criteria were whether the sample was clarified or stratified.…”