Effect of Nanofiber-Coated Surfaces on the Proliferation and Differentiation of Osteoprogenitors In Vitro

Abstract

The osteoconductive property of titanium (Ti) surfaces is important in orthopedic and dental implant devices. Surface modifications of Ti have been proposed to further improve osseointegration. In this study, three different materials, silicon (Si), silicon oxide (SiO(2)), and titanium oxide (TiO(2)), were used to construct nanofibers for surface coating of Ti alloy Ti-6Al-4 V (Ti alloy). MC3T3-E1 osteoprogenitor cells were seeded on nanofiber-coated discs and cultured for 42 days. DNA, alkaline phosphatase, osteocalcin, and mineralization nodules were measured using PicoGreen, enzyme-linked immunosorbent assay, and calcein blue staining to detect the attachment, proliferation, differentiation, and mineralization of MC3T3-E1 cells, respectively. The results demonstrated that the initial cell attachments on nanofiber-coated discs were significantly lower, although cell proliferation on Si and SiO(2) nanofiber-coated discs was better than on Ti alloy surfaces. TiO(2) nanofibers facilitated a higher cellular differentiation capacity than Ti alloy and tissue culture-treated polystyrene surfaces. Thus, surface modification using nanofibers of various materials can alter the attachment, proliferation, and differentiation of osteoprogenitor cells in vitro.

Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings

“…These thin films may be useful as resilient coatings on biomedical implants, where specific biofunctionality can be imparted to the implant surface through engineering of the modular engineered ELP. 41 …”

Heat or radiofrequency plasma glow discharge treatment of a titanium alloy stimulates osteoblast gene expression in the MC3T3 osteoprogenitor cell line

“…TiO 2 surfaces containing nanofibers [36] or nanotubes [37] facilitated a higher cellular differentiation capacity than surfaces devoid of nanostructures. Zhao et al [38] found that the microroughening of Ti6Al4V surfaces by grit-blasting with hydroxyapatite/beta tricalcium phosphate particles markedly increased the expression of osteocalcin but decreased alkaline phosphatase activity.…”

Multilayered electrospun fibrous meshes for restenosis-suppressing metallic stents

“…Nanofiber sheet has been widely applied to wound site and effectively delivered drugs and recovered wound site . The application was extended to coating materials of implanting devices such as orthopedic and dental implantation and bare metal stent . Flexible sheet that is compatible for coating materials and uniformly coated states on stent were kept even with expanding state of stent according to the polymers.…”

“…[11][12][13] The application was extended to coating materials of implanting devices such as orthopedic and dental implantation and bare metal stent. [14][15][16] Flexible sheet that is compatible for coating materials and uniformly coated states on stent were kept even with expanding state of stent according to the polymers. Also local drug delivery system of nanofiber made it possible of development of new type of drug-eluting stent compared with previous studies of drug-eluting stents.…”