Effect of NanometricLactobacillus plantarumin Kimchi on Dextran Sulfate Sodium-Induced Colitis in Mice

Abstract

Nanometric Lactobacillus plantarum (nLp) is a processed form of Lab. plantarum derived from kimchi and is 0.5-1.0 μm in size. This study was undertaken to determine the effect of nLp and kimchi plus nLp (K-nLp) on a dextran sulfate sodium (DSS)-induced mouse model of colitis. Animals fed nLp or K-nLp had longer colons, but lower colon weights per unit length than DSS controls. In addition, nLp- or K-nLp-fed animals showed lower levels of proinflammatory cytokines and inflammatory genes in serum and in colon tissues, lower populations of total bacteria, but higher populations of lactic acid bacteria in feces, and lower activities of fecal β-glucosidase and β-glucuronidase. Furthermore, these suppressive activities of nLp on colitis were equivalent to or higher than those of naive Lab. plantarum. Consequently, nLp was found to exhibit anticolitic effects, and the addition of nLp to kimchi was found to enhance the protective activity of kimchi against DSS-induced colitis. These results suggest that nLp might be an effective substitute for live probiotics and be useful as a functional ingredient with the anticolitic activity by the probiotic and food processing industries.

Immune-Enhancing Effect of Nanometric Lactobacillus plantarum nF1 (nLp-nF1) in a Mouse Model of Cyclophosphamide-Induced Immunosuppression

“…In vitro studies using splenocytes of BALB/c mice have shown that nLp-nF1 has immunostimulating activities, involving the production of interleukin (IL)-12 and interferon (IFN)-α [26][27][28]. Furthermore, administration of nLp-nF1 effectively attenuates dextran sulfate sodium (DSS)-induced colitis symptoms and DSS-azoxymethane-induced colorectal cancer [29,30]. These anti-inflammatory and tumor suppressive effects might be derived from the induction of T helper (Th) 1 cells by IL-12, and from the induction of tumor necrosis factor-alpha (TNF-α) production.…”

Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk

“…Generally, most isolates of Lactobacillus plantarum from fermented food were evaluated for resistance to acidic environments and to bile salt juice, antagonism against pathogens, and adhesion on epithelial cells in vitro. Other works have focused on its immune-regulatory property (Zago et al, 2011), anticolitis effect (Lee et al, 2015), and potential beneficial adverse effect on inflammatory bowel disease (Mileti et al, 2009) in vitro.…”

Probiotics as Potential Therapeutics for Colorectal Cancer

“…Recent studies implicate LAB in immune responses critical for CRC prevention and therapy [33]. LAB-mediated effects on the gut microbiome seem to focus specifically on responses to immune checkpoint blockade [34][35][36][37].…”

“…Inflammatory molecules are involved in various steps of carcinogenesis; thus, those with inflammatory conditions such as ulcerative colitis are at high risk of developing CRC [37]. L. plantarum reduces expression of proinflammatory cytokines and inflammatory genes and suppress inflammatory markers in a colitis mouse model [38].…”