Effect of nanoparticle based mrna delivery on modulation of inflammation in an osteochondral inflammation model

Basoli, Valentina; Li, Z.; Traweger, Andreas; Sanchez-Antequera, Yolanda; Paar, Christian; Rip, Jasper; Alini, Mauro; Grad, Sibylle

doi:10.1016/j.joca.2021.05.024

Cited by 1 publication

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“…It has been documented that abat is mainly responsible for the conversion of the inhibitory neurotransmitter 4-aminobutyric acid into succinate hemaldehydes, and finally succinate [ 35 ]. Succinate is regarded as a danger signal to promote the inflammatory response in the immune system, with increased levels in chronic inflammatory and metabolic diseases [ 36 ]. Therefore, the increase in succinate level induced by up-regulated abat in vivo indicated that the D. magna might be involved in immune metabolism, which proved that DES had a toxic effect on the organism at a high concentration.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Diethylstilbestrol in Daphnia Magna: Energy Metabolism and Growth Inhibition

Zhao

Guo

et al. 2023

Toxics

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With the widespread use of diethylstilbestrol (DES), it has become a common contaminant in the aquatic environment. It is toxic to a wide range of aquatic organisms, disrupting the water flea growth and further interfering with several ecosystem services. Nevertheless, the molecular mechanism of DES in water fleas is still unexplicit. In this study, the 21-day chronic test showed that a negative effect of growth and reproduction can be observed with DES exposure. Subsequently applied transcriptomic analysis illustrated the molecular mechanism in mode freshwater invertebrate Daphnia magna (D. magna) exposed to 2, 200, and 1000 μg·L−1 of DES for 9 days. Meanwhile, exposure to DES at 200 and 1000 μg·L−1 significantly restrains the growth (body length) and reproduction (first spawning time) of D. magna. Identified differentially expressed genes (DEGs) are majorly enriched relative to energy metabolism, lipid metabolism, the digestive system, transport and catabolism pathways which were remarkably changed. These repressed and up-regulated pathways, in relation to energy synthesis and metabolism, may be the reasons for the reduced body length and delayed first spawning time. Taken together, this study revealed that DES is a threat to D. magna in the aquatic environment and clarifies the molecular mechanism of the toxicity.

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Section: Discussionmentioning

confidence: 99%