Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae

Buchmann, Leandro; Frey, Wolfgang; Gusbeth, Christian; Ravaynia, Paolo S.; Mathys, Alexander

doi:10.1016/j.biortech.2018.09.124

Cited by 53 publications

(23 citation statements)

References 42 publications

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“…Scale-up approaches using nsPEF technology can benefit to a great extent from experience in the domain of conventional PEF processing (Buckow et al, 2010; Toepfl, 2011). However, PEF processing requires a multidisciplinary approach, including an understanding of innovative concepts within electrical engineering, fluid mechanics, and biology (Buchmann et al, 2018a,b, 2019c). The application of PEF to biological cells is based on the principle of electropermeabilization due to an induced transmembrane potential (Pauly and Schwan, 1959; Zimmermann et al, 1974; Schoenbach et al, 2004).…”

Section: Pulsed Electric Field Treatment In the Bio-based Industrymentioning

confidence: 99%

“…Initial studies of growth stimulation based on nsPEF processing yielded promising results for Arabidopsis thaliana in a batch system and different laboratories (Eing et al, 2009; Songnuan and Kirawanich, 2012). In recent experiments, the transfer from a batch system to a continuous nsPEF process was successful, resulting in a 13.1 ± 1.6% increase in Arthrospira platensis SAG 21.99 biomass (Buchmann et al, 2019c). Moreover, an increase of 18.8 ± 5.5% and 19.5 ± 6% in allophycocyanin and C-phycocyanin, respectively, components of the economically important blue colorant phycocyanin, was obtained.…”

Section: Pulsed Electric Field Treatment In the Bio-based Industrymentioning

confidence: 99%

“…Pulsed electric field (PEF) processing is a growing field in the area of electro-magnetic technologies for medical, environmental, and food applications (Toepfl et al, 2006b; Miklavčič et al, 2014; Postma et al, 2016; Raso et al, 2016; Buchmann et al, 2018b, 2019c). However, knowledge transfer and applications in bio-based industries (including yeast, lactobacilli, algae, and cell tissue production systems) have been limited.…”

Section: Introductionmentioning

confidence: 99%

“…nsPEF induces intracellular effects, distinct from the pronounced effects of conventional PEF on the cell membrane (Kotnik and Miklavčič, 2006; Chopinet and Rols, 2015). Thereby, innovative applications and novel process windows are possible, while similar components for both treatments in batch and continuous mode are required (Toepfl, 2011; Buchmann et al, 2019c). In both cases, the resulting electropermeabilization increases the mass transfer of molecules and ions (Toepfl et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

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Perspective on Pulsed Electric Field Treatment in the Bio-based Industry

Buchmann

Mathys

2019

Front. Bioeng. Biotechnol.

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The bio-based industry is urged to find solutions to meet the demands of a growing world population. In this context, increased resource efficiency is a major goal. Pulsed electric field (PEF) processing is a promising technological solution. Conventional PEF and the emerging area of nanosecond PEF (nsPEF) have been shown to induce various biological effects, with nsPEF inducing pronounced intracellular effects, which could provide solutions for currently faced challenges. Based on the flexibility and continuous operation of PEF and nsPEF processing, the technology can be integrated into many existing cultivation systems; its modularity provides an approach for inducing specific effects. Depending on the treatment conditions, selective inactivation, continuous extraction without impeding cell viability, as well as the stimulation of cell growth and/or cellular compound stimulation are potential applications in the bio-based industry. However, continuous treatment currently involves heterogeneous energy inputs. Increasing the homogeneity of PEF and nsPEF processing by considering the flow and electric field heterogeneity may allow for more targeted effects on biological cells, further increasing the potential of the technology for bio-based applications. We provide an overview of existing and potential applications of PEF and nsPEF and suggest that theoretical and practical analyses of flow and electric field heterogeneity may provide a basis for obtaining more targeted effects on biological cells and for further increasing the bio-based applications of the technology, which thereby could become a key technology for circular economy approaches in the future.

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Section: Pulsed Electric Field Treatment In the Bio-based Industrymentioning

confidence: 99%

Section: Pulsed Electric Field Treatment In the Bio-based Industrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Perspective on Pulsed Electric Field Treatment in the Bio-based Industry

Buchmann

Mathys

2019

Front. Bioeng. Biotechnol.

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“…Further potential applications of nsPEFs include growth stimulation in plants 11 , boosting the yield of edible mushrooms 12 , the chemical free processing and preservation of food (for review, see 13 ). For microalgae, they have been found to induce an increase in dry weight 14 , 15 and even to trigger oxidative burst and palmella stage formation in C. reinhardtii 6 .…”

Section: Introductionmentioning

confidence: 99%

Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis

Bai

Gusbeth

Frey

et al. 2020

Sci Rep

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Nanosecond pulsed electric fields (nsPEFs) have been extensively studied with respect to cellular responses. Whether nsPEFs can regulate gene expression and to modulate the synthesis of valuable compounds, has so far been only tested in the context of apoptosis in cancer cells. We used the unicellular algae Haematococcus pluvialis as system to test, whether nsPEFs could alter gene expression and to promote the biosynthesis of astaxanthin. We find that nsPEFs induce a mild, but significant increase of mortality up to about 20%, accompanied by a moderate increase of astaxanthin accumulation. Steady-state transcript levels of three key genes psy, crtR-b and bkt 1 were seen to increase with a maximum at 3 d after PEF treatment at 50 ns. Pulsing at 25 ns reduce the transcripts of psy, crtR-b from around day 2 after the pulse, while those of bkt 1 remain unchanged. By blocking the membrane-located NADPH oxidase RboH, diphenylene iodonium by itself increased both, the levels of astaxanthin and transcripts of all three biosynthetic genes, and this increase was added up to that produced by nsPEFs. Artificial calcium influx by an ionophore did not induce major changes in the accumulation of astaxanthin, nor in the transcript levels, but amplified the response of crtR-b to nsPEFs at 25 ns, while decreased in 50 ns treatment. When Ca2+ influx was inhibited by GdCl3, the transcript of psy and bkt 1 were decreased for both 25 ns and 50 ns treatments, while crtR-b exhibited an obvious increase for the 25 ns treatment. We interpret these data in a working model, where nsPEFs permeabilise plasma and chloroplast membrane depending on pulse duration leading to a differential release of plastid retrograde signaling to the nucleus.

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Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses

Porcher

Wilmot

Bonnet

et al. 2023

Bioelectromagnetics

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The biological effects of exposure to electromagnetic fields due to wireless technologies and connected devices are a subject of particular research interest. Ultrashort high‐amplitude electromagnetic field pulses delivered to biological samples using immersed electrodes in a dedicated cuvette have widely demonstrated their effectiveness in triggering several cell responses including increased cytosolic calcium concentration and reactive oxygen species (ROS) production. In contrast, the effects of these pulses are poorly documented when electromagnetic pulses are delivered through an antenna. Here we exposed Arabidopsis thaliana plants to 30,000 pulses (237 kV m−1, 280 ps rise‐time, duration of 500 ps) emitted through a Koshelev antenna and monitored the consequences of electromagnetic fields exposure on the expression levels of several key genes involved in calcium metabolism, signal transduction, ROS, and energy status. We found that this treatment was mostly unable to trigger significant changes in the messenger RNA accumulation of calmodulin, Zinc‐Finger protein ZAT12, NADPH oxidase/respiratory burst oxidase homolog (RBOH) isoforms D and F, Catalase (CAT2), glutamate‐cystein ligase (GSH1), glutathione synthetase (GSH2), Sucrose non‐fermenting‐related Kinase 1 (SnRK1) and Target of rapamycin (TOR). In contrast, Ascorbate peroxidases APX‐1 and APX‐6 were significantly induced 3 h after the exposure. These results suggest that this treatment, although quite strong in amplitude, is mostly ineffective in inducing biological effects at the transcriptional level when delivered by an antenna. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.

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Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae

Cited by 53 publications

References 42 publications

Perspective on Pulsed Electric Field Treatment in the Bio-based Industry

Perspective on Pulsed Electric Field Treatment in the Bio-based Industry

Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis

Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses

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