Effect of Narrow-band Ultraviolet B Phototherapy on p63 and MicroRNA (miR-21 and miR-125b) Expression in Psoriatic Epidermis

Gu, Xiaolian; Nylander, Elisabet; Coates, Philip J.; Nylander, Karin

doi:10.2340/00015555-1086

Cited by 44 publications

(18 citation statements)

References 33 publications

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“…In this study, q-PCR showed high degrees of sensitivity and accuracy for measuring the expression of these genes. Immunohistochemistry has confirmed the PCR results on the protein level, showing that these methods are similar to previous dermatological studies [12,13,16]. mRNA levels and protein expression corresponded in our current study.…”

Section: Discussionsupporting

confidence: 92%

Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

et al. 2012

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The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5), and their mRNA expression. This was done in the skin of five breeds of dogs including seven poodles, six golden retrievers, six Shih Tzus, four pugs, and four Labrador retrievers. Epidermal thickness of the stratum corneum and nucleated epidermal layer was significantly different. The greatest thickness was observed in the digital web area and the thinnest epidermis was in the axilla. Epidermal thickness was also significantly different between the breeds (p ＜ 0.05). Immunohistochemical staining scores revealed significant decreases of involucrin, filaggrin, and keratin 10 in the ventral and weight-bearing sites, and a relative increase of keratin 5 (p ＜ 0.05). q-PCR analysis showed that their the levels of mRNA were positively correlated with expression of the corresponding proteins in skin samples (p ＜ 0.05). The present study is the first to report the relationship between epidermal gene expression and histologic morphology of the skin in normal dogs. Further studies will be essential to fully understand the pathogenesis of skin barrier dysfunctions in canines.

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Section: Discussionsupporting

confidence: 92%

Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

et al. 2012

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“…Also there was decrease in miR146a level in patients treated with NB-UVB in this study; this was in accordance with the results of Gu et al [ 24 ]. This was due to the effect of NB-UVB leading to cell cycle arrest, T cell apoptosis, and apoptosis upon DNA damage as stated by Menter et al [ 25 ] and Gu et al [ 24 ].…”

Section: Discussionsupporting

confidence: 93%

Effect of Narrow-Band Ultraviolet B Phototherapy and Methotrexate on MicroRNA (146a) Levels in Blood of Psoriatic Patients

Ele-Refaei

Elesawy

2015

Dermatology Research and Practice

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Background. Recently, some miRNAs have been proven to show aberrant expression in psoriasis and play a role in the pathogenesis of the disease. Objective. To find out whether NB-UVB or methotrexate treatment affects whole blood levels of human miRNA (146a) in patients with psoriasis and demonstrate its correlation with disease severity. Methods. Blood samples were obtained from healthy control and from psoriatic patients before and 12 weeks after treatment with NB-UVB, methotrexate. Quantification of human miRNA (146a) by Real Time PCR (RT-PCR). Results. Blood human miRNA (146a) levels were higher in patients with psoriasis than those in healthy controls (P = 0.001); it had no significant positive relation with PASI scores in patients (r = 0.2, P = 0.107). Real Time PCR showed that, after 12 weeks of treatment with NB-UVB phototherapy or treatment with methotrexate, there was significantly decreased level of miR146a (P = 0.001; P = 0.002, resp.). Conclusion. The expression of miRNA146a is increased in whole blood samples from psoriasis patients, so we can evaluate its possibility to work as a future therapeutic objective in the treatment of psoriasis. With these markers, it is able to screen therapeutics effect or changes to a further aggressive treatment for psoriasis.

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“…[41] Our results clearly showed expression of all three miRNAs studied to be altered in tumor tissue compared to clinically normal tissue from the same patients. In accordance with previous studies, miR-21 was significantly upregulated in tumors compared to control samples, whereas miR-125b and miR-203 showed significantly decreased expression in tumor samples compared to controls, indicative of a role for all these miRNAs in this tumor type.…”

Section: Discussionmentioning

confidence: 65%

Subsite-based alterations in miR-21, miR-125b, and miR-203 in squamous cell carcinoma of the oral cavity and correlation to important target proteins

et al. 2012

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Background:MicroRNAs (miRNAs) are small noncoding RNA molecules with an essential role in regulation of gene expression. miRNA expression profiles differ between tumor and normal control tissue in many types of cancers and miRNA profiling is seen as a promising field for finding new diagnostic and prognostic tools.Materials and Methods:In this study, we have analyzed expression of three miRNAs, miR-21, miR-125b, and miR-203, and their potential target proteins p53 and p63, known to be deregulated in squamous cell carcinoma of the head and neck (SCCHN), in two distinct and one mixed subsite in squamous cell carcinoma in the oral cavity.Results:We demonstrate that levels of miRNA differ between tumors of different subsites with tongue tumors showing significant deregulation of all three miRNAs, whereas gingival tumors only showed significant downregulation of miR-125b and the mixed group of tumors in tongue/floor of the mouth showed significant deregulation of miR-21 and miR-125b. In the whole group of oral squamous cell carcinoma (SCC), a significant negative correlation was seen between miR-125b and p53 as well as a significant correlation between TP53 mutation status and miR-125b.Conclusion:The present data once again emphasize the need to take subsite into consideration when analyzing oral SCC and clearly show that data from in vitro studies cannot be transferred directly to the in vivo situation.

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Effect of Narrow-band Ultraviolet B Phototherapy on p63 and MicroRNA (miR-21 and miR-125b) Expression in Psoriatic Epidermis

Cited by 44 publications

References 33 publications

Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

Effect of Narrow-Band Ultraviolet B Phototherapy and Methotrexate on MicroRNA (146a) Levels in Blood of Psoriatic Patients

Subsite-based alterations in miR-21, miR-125b, and miR-203 in squamous cell carcinoma of the oral cavity and correlation to important target proteins

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