Effect of Natural and Pretreated Soybean Hulls on Enzyme Production by <i>Trichoderma reesei</i>

Coffman, Anthony M.; Li, Qian; Ju, Lu‐Kwang

doi:10.1007/s11746-014-2480-8

Cited by 28 publications

(15 citation statements)

References 26 publications

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“…39 U g ‐1 hulls, which is comparatively higher than the 0.58 U g ‐1 hulls obtained by culturing Trichoderma reesei in shake flasks on the same substrate as the main carbon source . Soybean hulls also induced production of XYL activity in A. sojae , although yields were considerably lower compared with PG and with other literature reports . Ambient pH is known to play an important role in XYL production in filamentous ascomycetes, but no further studies concerning xylan‐degrading enzymes were conducted in the present work.…”

Section: Discussioncontrasting

confidence: 64%

“…The final PG yield per gram of soybean hulls calculated was 1. 39 U g ‐1 hulls, which is comparatively higher than the 0.58 U g ‐1 hulls obtained by culturing Trichoderma reesei in shake flasks on the same substrate as the main carbon source . Soybean hulls also induced production of XYL activity in A. sojae , although yields were considerably lower compared with PG and with other literature reports .…”

Section: Discussionmentioning

confidence: 54%

“…29 Soybean hulls also induced production of XYL activity in A. sojae, although yields were considerably lower compared with PG and with other literature reports. 28,29 Ambient pH is known to play an important role in XYL production in filamentous ascomycetes, 30 but no further studies concerning xylan-degrading enzymes were conducted in the present work. optimizing the culture conditions for PG production by a design-of-experiments approach, the process was successfully transferred to bioreactors, where the PG yields obtained per gram of soybean hulls were, to the best of our knowledge, among the greatest reported.…”

Section: Discussionmentioning

confidence: 56%

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Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

Fratebianchi¹,

Acosta²,

Cavalitto³

2017

J of Chemical Tech & Biotech

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BACKGROUND Soybean hulls result from the processing of the bean for producing oil and protein products. This by‐product generated massively in America has virtually no commercial value, so substantial effort is being applied to its exploitation for generating value‐added goods. This work evaluates soybean hulls as inducer of the production of pectinolytic enzymes, through optimization studies regarding polygalacturonase production by Aspergillus sojae in submerged cultures. RESULTS A 2‐fold improvement in polygalacturonase yield was found by varying the initial pH of the culture in a very narrow acid pH range (2.40–2.80). The optimized fermentation process was successfully transferred to stirred‐tank bioreactors in terms of volumetric productivity, and final polygalacturonase yields were 42 U mL‐1 and 1.39 U g‐1 soybean hulls, which are among the highest reported with this by‐product. Morphological characterization of A. sojae during cultivation showed that the fungus mainly developed in dispersed mycelia at initial pH of 2.40–2.80 while, conversely, fungal pellets predominated in cultures performed at initial pH of 5.40. CONCLUSION High enzyme titers are possibly connected to the formation of dispersed mycelia, as well as to acid‐induced expression of the respective gene/s. It is foreseen that this data will be helpful regarding the production of fungal pectinases or other acid‐induced enzymes. © 2017 Society of Chemical Industry

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 56%

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Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

Fratebianchi¹,

Acosta²,

Cavalitto³

2017

J of Chemical Tech & Biotech

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“…High pH increases xylanase and cellulase and low pH increases polygalacturonase production [48] 1. exoglucanase Cel7A overproduction. [27] Depletion of the interfering genes, like cre1, can increase the cellulase amount.…”

Section: Cellulase Production By T Reeseimentioning

confidence: 99%

Trichoderma reesei, a superior cellulase source for industrial applications

Keshavarz

Khalesi

2016

Biofuels

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Trichoderma reesei, a superior cellulase source for industrial applications, is a genus of filamentous fungi with an exceptional potential to produce various enzymes and proteins including cellulases, hemicellulases, and hydrophobins. Growing on easy-access low-cost substrates, as well as a broad range of products produced by T. reesei, represent this genus as the first option for bio-industries to produce required enzymes and proteins with an efficient direction of bioprocess. Recently, many researchers have made efforts to improve the yield of T. reesei cellulases using recombinant strains, optimization of the substrates, and applying the synergistic effects of co-cultivation using different microorganisms. The current findings may help industries such as biofuel producers to reduce the cost of cellulase production by up to 40%. In this review, recent advances in last five years regarding the improvement in cellulase productivity using T. reesei have been pointed out and the highlights have been summarized.

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“…Fungal fermentation to produce enzyme broth was conducted according to process reported from current laboratory [29]. Enzyme solutions used in this study were prepared from the cell-free supernatants collected by centrifugation of the fermentation broths at 8000 rpm (7440×g) for 10 min.…”

Section: Enzyme Solutionsmentioning

confidence: 99%

Optimization of Enzymatic Process Condition for Protein Enrichment, Sugar Recovery and Digestibility Improvement of Soy Flour

Loman

2016

J Americ Oil Chem Soc

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IntroductionSoybean meal is used as a protein supplement for animal feed and fish meal alternative [1]. Soy protein has a well balanced and relatively constant amino acid profile [2]. There is increasing interest in soy protein-based food and supplement to animal protein. The US Food and Drug Administration (FDA) has approved the claim that soy protein helps reduce the risk of coronary heart disease [3]. Soy protein products are typically made from defatted soy flour or meal, which contains ~50 % protein on a weight basis. Carbohydrate (30-35 %) is the main non-protein component in defatted soy flour [4]. Despite its high content, soy carbohydrate has not yet drawn serious attention for valueadded uses. Soy flour carbohydrate is composed of ~15 % sugars (mostly di-to oligo-saccharides like sucrose, raffinose and stachyose) and 15-20 % non-starch polysaccharides (NSP) [5,6]. The carbohydrate can reduce the value of soy products for two reasons: it lowers the protein content, potentially to a level not high enough for use in certain animal feeds; and the NSP and galacto-oligosaccharides have anti-nutritive concerns [7].Monogastric animals lack the endogenous enzymes required for digesting these NSP and oligosaccharides [7]. Adverse effects of elevated levels of NSP have been reported and reviewed. They are associated with the viscous nature of NSP, their physiological and morphological effects on digestive tract, and their interactions with epithelium, mucus and gut microflora [8][9][10][11]. NSP can also negatively influence the metabolism and utilization of nutrients like glucose, lipid, amino acids and minerals [12]. This is because NSP cause viscosity increase of the digesta, leading to a lower rate of gastric emptying and the depressed nutrient absorption [13]. NSP also bind nutrients and form complexes with digestive enzymes and some regulatory Abstract Soy protein is a valuable nutritional supplement for food and animal feed. While protein constitutes ~50 % of defatted soy flour (SF), it coexists with complex carbohydrates (30-35 %) which may have anti-nutritional effects. An enzymatic process can remove the carbohydrate and produce protein-enriched soy products. The hydrolysate with monomerized carbohydrates is valuable fermentation feedstock. In this study, Aspergillus niger and Trichoderma reesei enzymes were compared for use in the process. Effects of pH (3.2-6.4), temperature (40-60 °C), enzyme-to-SF ratio (0-2 ml/g) and SF loading (150-350 g/l) were evaluated for the enzymatic conversion of SF carbohydrate to reducing sugar (Y RS ) and total soluble carbohydrate (Y TC ) in the hydrolysate. Effects of these single factors and interactions between factors were investigated. Optimal pH and temperature were similar for both enzymes: pH 4.8 and 50-51 °C for Y TC , and pH 5.1-5.2 and 48-51 °C for Y RS . The two enzymes also gave similar protein contents in resultant soy protein concentrates, i.e., 74-75 % with 2 ml/g enzyme broth and 150 g/l SF, which were higher than the 64-68 % protein in commercial concen...

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Effect of Natural and Pretreated Soybean Hulls on Enzyme Production by Trichoderma reesei

Cited by 28 publications

References 26 publications

Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine‐tuning of ambient pH

Trichoderma reesei, a superior cellulase source for industrial applications

Optimization of Enzymatic Process Condition for Protein Enrichment, Sugar Recovery and Digestibility Improvement of Soy Flour

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