Effect of neonatal exposure to endocrine-active compounds on epigenetic regulation of gene expression in corpus luteum of gilts

Witek, Patrycja; Grzesiak, Małgorzata; Kotula–Balak, Małgorzata; Koziorowski, Marek; Słomczyńska, Maria; Knapczyk-Stwora, Katarzyna

doi:10.1016/j.theriogenology.2020.10.010

Cited by 4 publications

(3 citation statements)

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“…In recent studies we have examined the consequences of neonatal exposure to environmental endocrine‐active compounds (EACs) with androgenic/antiandrogenic and estrogenic/antiestrogenic activities, including MXC, for luteal function in adult pigs. We showed that DNA methylation and protein levels for compounds of the miRNA biogenesis machinery changed in response to neonatal EACs treatment (Witek et al, 2021). Moreover, we demonstrated an altered miRNA profile in the luteal tissue of pigs that were exposed to androgens or antiandrogens in the neonatal life (Knapczyk‐Stwora et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis

Witek

Enguita

Grzesiak

et al. 2021

Molecular Reproduction Devel

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This study investigated the effects of neonatal exposure to methoxychlor (MXC), a synthetic organochlorine used as an insecticide with estrogenic, antiestrogenic, and antiandrogenic activities, on luteal function in pigs. Piglets were injected subcutaneously with MXC (20 μg/kg body weight) or corn oil (control) between postnatal Days 1 and 10 (N = 5/group). Corpora lutea from sexually mature gilts were examined for luteal steroid and prostaglandin concentrations and processed for total RNA isolation and subsequent RNA sequencing. Intra‐luteal concentrations of androstenedione and prostaglandin E2 were greater, while that of estrone was lower when compared to control. Fifty‐three differentially expressed (DE) microRNAS (miRNAs) (p‐adjusted <.05 and log2(fold change) ≥.5) and 359 DE genes (p‐adjusted <.05 and log2(fold change) ≥1) were identified in luteal tissue in response to neonatal MXC treatment. MXC was found to affect the expression of genes related to lipogenesis, steroidogenesis, membrane transport, immune response, cell signaling and adhesion. These results suggest an earlier onset of structural luteolysis in pigs caused by MXC actions in neonates. Since negative correlation analysis showed the potential interactions of miRNAs with specific messenger RNAs, we propose that these miRNAs are potential mediators of the long‐term MXC effect on the CL function in pigs.

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Section: Introductionmentioning

confidence: 99%

Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis

Witek

Enguita

Grzesiak

et al. 2021

Molecular Reproduction Devel

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“…Moreover, a successful gestation is associated with high levels of production of progesterone by the pregnant female (SOEDE; LANGENDIJK; KEMP, 2011; STOCCO; TELLERIA; GIBORI, 2007). In this sense, several studies are investigating the role of environmental factors (non-genetic) that may be related to the modulation of the gene expression of this gland (WITEK et al, 2020).…”

Section: Physiology and Regression Of The Corpus Luteummentioning

confidence: 99%

“…[116] Long-term effects of endocrine-active compounds on corpus luteum of swine females exposed during early life period. [117] Porcine embryos Investigation of the effects of histone deacetylase inhibitors on the in vitro development of porcine embryos derived from somatic cell nuclear transfer.…”

Section: Giltsmentioning

confidence: 99%

>b<Molecular markers in ovaries of female pigs with different levels of welfare, in the pre-mating period>/b<

Silva¹

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writingreview and editing) for their collaboration. Likewise, for the development of the manuscript "A challenge with lipopolysaccharide (LPS) on the day of estrus can compromise gene expression of the corpus luteum of gilts", I am grateful for the collaboration and efforts of: Germana Vizzotto Osowski (software and visualization), Dr. Guilherme Pugliesi (methodology, laboratory equipment, and resources), MSc. Luana Alves (conceptualization and investigation), Dr. Mariana Groke Marques (methodology and resources), Dr. Priscila Assis Ferraz (investigation), and Dr. Ricardo Zanella (methodology). I thank Biorender (biorender.com) platform for creating figures.

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Pesticides

González-Alvarez,

Timme,

Keating

2024

Reference Module in Biomedical Sciences

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Effect of neonatal exposure to endocrine-active compounds on epigenetic regulation of gene expression in corpus luteum of gilts

Cited by 4 publications

References 42 publications

Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis

Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis

>b<Molecular markers in ovaries of female pigs with different levels of welfare, in the pre-mating period>/b<

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