Effect of nerve growth factor (NGF) on the development of preimplantation rabbit embryos in vitro

Pei, Yanxi

doi:10.1007/s11259-009-9325-1

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…In addition, in this study, when testing a higher dose than found in SP, β-NGF did not affect fertility, early embryo development/implantation, gestation and delivery. Indeed, studies have shown that β-NGF enhances embryo development in vitro [61]. We observed only one ovulating doe (but not pregnant) in the negative control group.…”

Section: Discussionmentioning

confidence: 47%

Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits

et al. 2019

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In some induced-ovulating species, beta nerve growth factor (β-NGF) has important roles in ovulation, though data for rabbits are still inconclusive. In this study we first synthesized functional recombinant β-NGF from rabbit tissue (rrβ-NGF) to address the following objectives: 1) to compare rabbit β-NGF amino acid sequence with those of other induced- or spontaneous-ovulating species; 2) to assess the effects of rrβ-NGF on rabbit sperm viability and motility, and 3) to examine the in vivo ovulation inducing effect of rrβ-NGF added to the seminal dose in rabbit does. The NGF gene in rabbit prostate tissue was sequenced by Rapid Amplification of cDNA Ends and annotated in GenBank (KX528686). Recombinant rβ-NGF was produced in CHO cells and purified by affinity chromatography. Once confirmed by Western blotting and mass spectrometry (MALDI-TOF) that the amino acid sequence of the recombinant protein corresponded to β-NGF, its functionality was validated in PC12 cells in a successful dose-response study over 8 days. The amino acid sequence of prostate rabbit NGF differed to that of other species mainly in its receptor binding sites. In all the spontaneous ovulating species examined, compared with rabbit, alanine and proline residues, which interact with the high-affinity receptor, were replaced by a serine. In rabbits, asparagine and methionine were substituted by lysine at the low-affinity receptor binding site. In time- and dose-response experiments, the in vitro addition of rrβ-NGF to the ejaculate did not affect sperm viability whereas sperm motility parameters were enhanced by the addition of 1 μg/mL of the neuropeptide. Addition of this same concentration of rrβ-NGF to the seminal dose administered via the intravaginal route in does induced ovulation with a delayed LH peak, leading to a plasma progesterone increase, gestation and delivery. Our findings suggest that rrβ-NGF could be a useful option for biotechnological and reproduction assisted techniques in rabbits but further studies are needed.

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Section: Discussionmentioning

confidence: 47%

Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits

et al. 2019

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“…Embryos are delayed at the ampulla-isthmus junction until 48 h post-mating and then pass through the isthmus portion after 70 h post-mating to finally pass through the utero-tubal junction and enter the uterus [42]. The analyses of NGF expression showed an upregulation in the isthmus at 36, 68, and 72 h post-mating, suggesting that embryos are naturally exposed to NGF during its passage along the isthmus before implantation, probably to enhance embryo hatching rates in a similar way as reported previously in a rabbit in vitro embryo development study [43]. However, the mechanisms by which NGF exerts such actions are still unclear.…”

Section: Discussionsupporting

confidence: 78%

Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits

Gardela

Jauregi-Miguel

Martínez

et al. 2020

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Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (n = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (n = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial CTEN and NGF by NM, but not by SP-AI. The findings suggest that the NGF gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development.

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“…In rabbit female, the presence of the β-NGF and its receptors in several types of ovarian cells (theca, granulosa and cumulus), corpus luteum (Zerani et al, 2021) and in uterus and oviduct (Maranesi et al, 2018, García-García, 2018b involves important roles in this reproductive system, such as in folliculogenesis and ovulation (García-García et al, 2020), as well as in embryo development (Pei, 2010). In addition, β-NGF could be a powerful stimulator of prostaglandins biosynthesis by the rabbit uterus, as it induces the in vitro synthesis secretion of both PGF2a and PGE2 (Maranesi et al, 2016).…”

Section: Sp B-ngfmentioning

confidence: 99%

Physiology and modulation factors of ovulation in rabbit reproduction management

et al. 2021

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Rabbit is an induced ovulatory species, so ovulation takes place after mating. Traditionally, exogenous and synthetic hormonal factors (administered by intramuscular and intravaginal route) such as GnRH and analogues, or different physical procedures (i.e. stimulation by intravaginal cannula) have been used to induce ovulation in females when artificial insemination is applied in rabbit farms. Restriction and public rejection of the use of hormones is leading to the study of the seminal plasma components with potential action on ovulation induction. The aim of the present review is to collect and summarise the strategies used in recent years to trigger ovulation and improve rabbit fertility management with respect to more animal-friendly manipulation methods. Furthermore, special attention has been paid to the use of a semen component (as endogen molecule) such as beta nerve growth factor (β-NGF) in male and female rabbit reproductive physiology. This neurotrophin and its receptors (TrKA and p75NTR) are abundantly distributed in both male and female rabbit reproductive tracts, and it seems to have an important physiological role in sperm maturation and behaviour (velocity, apoptosis and capacitation), as well as a modulatory factor of ovulation. Endogen β-NGF is diluted in the seminal doses with the extenders; hence it could be considered an innovative and alternative strategy to avoid the current exogenous (by intramuscular route) and stressful hormonal treatments used in ovulation induction. Their addition in seminal dose could be more physiological and improve animal welfare in rabbit farms.

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Effect of nerve growth factor (NGF) on the development of preimplantation rabbit embryos in vitro

Cited by 9 publications

References 40 publications

Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits

Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits

Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits

Physiology and modulation factors of ovulation in rabbit reproduction management

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