Effect of Nerve Growth Factor and Dibutyryl Cyclic AMP on Biopterin and Catecholamine Levels of PC12h Pheochromocytoma Cells

Nakanishi, Nobuo; Onozawa, Satoshi; Matsumoto, Reiko; Nakamura, Masako; Imagawa, Shogo; Yamada, Shozo

doi:10.1515/pteridines.1991.3.12.67

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Catecholamines were extracted by a brief sonication (5 s at 0°C) of the cells in 0.5 ml of 0.1 M perchloric acid (PCA), 1 mM EDTA, and 0 .1% sodium bisulfite . The sonicate was centrifuged to precipitate proteins, and a 10-Ml sample of the supernatant was analyzed on an HPLC apparatus equipped with an electrochemical detector (Coulochem model 5100A) (Nakanishi et al, 1992) . The precipitate was dissolved in 1 M NaOH and used for protein content determination by the method of Bradford (1976) .…”

Section: Catecholamine Quantificationmentioning

confidence: 99%

“…In the course of our study on the effects of nerve growth factor and cAMP on tetrahydrobiopterin and catecholamine metabolism in pheochromocytoma PC 12 cells, including PC 12h, we observed a decrease in the cellular dopamine (DA) content caused by dibutyryl cAMP (dBcAMP) (Suzuki et al ., 1988 ;Nakanishi et al ., 1990Nakanishi et al ., , 1991Nakanishi et al ., , 1992 . dBcAMP as well as nerve growth factor increased the levels of both DOPA and tetrahydrobiopterin, an essential and subsaturating cofactor of tyrosine hydroxylase (Nagatsu et al ., 1964) .…”

mentioning

confidence: 99%

See 1 more Smart Citation

Cyclic AMP‐Dependent Modulation of Vesicular Monoamine Transport in Pheochromocytoma Cells

Nakanishi

Onozawa

Matsumoto

et al. 1995

Journal of Neurochemistry

View full text Add to dashboard Cite

Cyclic AMP (cAMP) is well known to enhance tyrosine hydroxylase activity in PC12 cells. We were able to demonstrate, however, that the cellular dopamine level in PC12 was lowered by dibutyryl cAMP. Furthermore, the decrease in the cellular level of dopamine was accompanied by about a 10‐fold increase in the medium. The aim of this work was to elucidate the effect of cAMP on catecholamine transport. Dibutyryl cAMP did not induce exocytotic release of norepinephrine but rather inhibited its uptake. As with forskolin and cholera toxin, physiological signaling molecules such as vasoactive intestinal polypeptide (VIP) and AMP, for which PC12 cells are known to have receptors linked to activation of adenylate cyclase, also inhibited norepinephrine uptake. The inhibitory effects of dibutyryl cAMP, VIP, and AMP were dose dependent, and EC50 values were estimated to be 100 µM, 10 nM, and 1.0 µM, respectively. The inhibition profile of dibutyryl cAMP over the time course of norepinephrine uptake was biphasic: Inhibition became clearly detectable after the cytosolic pool of norepinephrine had been saturated. This profile is similar to that of reserpine. Nomifensine, however, inhibited uptake at a rather constant rate throughout the entire time course. The ATP‐dependent serotonin uptake by digitonin‐permeabilized cells was lowered to ∼50% that of the control by dibutyryl cAMP treatment before permeabilization, indicating inhibition of vesicular monoamine transport. This effect was also dependent on a dibutyryl cAMP concentration with an EC50 of ≤100 µM. These results suggest that cAMP may be capable of elevating extracellular dopamine levels in the nervous system by inhibiting its translocation into storage vesicles while enhancing its synthesis in the cytosol. Moreover, endogenous neurotransmitters such as VIP, AMP, and adenosine may act as intrinsic antidepressants via the cAMP pathway.

show abstract

Section: Catecholamine Quantificationmentioning

confidence: 99%

mentioning

confidence: 99%

Cyclic AMP‐Dependent Modulation of Vesicular Monoamine Transport in Pheochromocytoma Cells

Nakanishi

Onozawa

Matsumoto

et al. 1995

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

Long-Term Treatment of PC12 Pheochromocytoma with Dibutyryl Cyclic Amp Increases Biopterin Content in the Cells but Decreases that in the Medium

Nakanishi

Onozawa

Isono

et al. 1993

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Effects of protein kinase inhibitors and protein phosphatase inhibitors on cyclic AMP‐dependent down‐regulation of vesicular monoamine transport in pheochromocytoma PC12 cells

et al. 1995

View full text Add to dashboard Cite

Cyclic AMP down-regulates vesicular monoamine transport in PC12 cells and thereby decreased catecholamine reuptake from the extracellular fluid. We examined the effects of protein kinase inhibitors and protein phosphatase inhibitors on this cAMP action. Treatment of cells with a protein kinase inhibitor, K252a, increased vesicular amine transport and cellular amine uptake, thereby antagonizing the regulatory action of cAMP. In contrast, a protein phosphatase inhibitor, okadaic acid, had the opposite effect on the amine transport, i.e. it enhanced the cAMP action. These results suggest the involvement of a protein phosphorylation process in the cAMP-dependent modulation of vesicular monoamine transport.

show abstract

Effect of Nerve Growth Factor and Dibutyryl Cyclic AMP on Biopterin and Catecholamine Levels of PC12h Pheochromocytoma Cells

Cited by 3 publications

References 0 publications

Cyclic AMP‐Dependent Modulation of Vesicular Monoamine Transport in Pheochromocytoma Cells

Cyclic AMP‐Dependent Modulation of Vesicular Monoamine Transport in Pheochromocytoma Cells

Long-Term Treatment of PC12 Pheochromocytoma with Dibutyryl Cyclic Amp Increases Biopterin Content in the Cells but Decreases that in the Medium

Effects of protein kinase inhibitors and protein phosphatase inhibitors on cyclic AMP‐dependent down‐regulation of vesicular monoamine transport in pheochromocytoma PC12 cells

Contact Info

Product

Resources

About