Effect of Neuraminidase Treatment on the Biological Activity of Highly Purified Ovine FSH and LH n i Hypophysectomized Immature Male and Female Rats

Grimek, H.J.; Nuti, L. C.; Nuti, K. M.; McShan, W. H.

doi:10.1210/endo-98-1-105

Cited by 16 publications

(6 citation statements)

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“…However, the injections of FSH (0-4 mg Armour Standard daily for 4 days) had not significantly increased the serum levels of several steroids above the basal levels found in control hypophysectomized animals (Table 3) ; this lack of a steroidogenic response to FSH is in contrast to other studies using immature rats and a short period between hypophysectomy and hormone treatment (Nuti, McShan & Meyer, 1974;Grimek, Nuti, Nuti & McShan, 1976). FSH thus stimulated the growth of follicles in the absence of an increase in steroidogenesis.…”

Section: Resumption Offollicular Growth In Hypophysectomized Ratsmentioning

confidence: 59%

Normal and abnormal follicular growth in mouse, rat and human ovaries

Edwards¹,

Fowler²,

Gore-Langton³

et al. 1977

Reproduction

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We wish to present an analysis of the data gained by a group of workers studying various aspects of ovarian activity. Our work has been concerned primarily with two aspects of the development of follicles: the initiation and control of follicular growth, and differentiation and steroidogenesis in follicles approaching ovulation. The scope of our contributions spans the lifetime of the female from the early stages of growth in the fetal ovary to the final stages of follicular development in ageing females and includes both animal and clinical studies. The results are given in two main sections: follicular growth, and normal and abnormal differentiation and steroidogenesis in follicles approaching ovulation. Several aspects of follicular growth are considered in the discussion. Follicular growthIs there a regularity in the formation and utilization offollicles ?A full understanding of follicular growth in the adult ovary cannot be gained until the factors governing the establishment offollicles in the immature ovary are clarified. Plentiful evidence has shown how follicles are formed in gradients in the fetal or neonatal ovary ; small follicles containing an oocyte in dictyotene are identified in some areas of the cortical cords, whereas oocytes still in early prophase of meiosis or oogonia in mitosis can be found in other areas. We suspect that this sequential formation of follicles is reflected in their future utilization in the adult female as they begin growth at various times during the lifespan. Such regularity in the formation and utilization offollicles was suggested by the results of studies on chiasma frequency in oocytes and spermatocytes taken from mice of different ages (Henderson & Edwards, 1968). A steady decline was found in the number of chiasmata in oocytes, and chiasma position became located towards the ends of the chromosomes as the female aged (Text- fig.

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Section: Resumption Offollicular Growth In Hypophysectomized Ratsmentioning

confidence: 59%

Normal and abnormal follicular growth in mouse, rat and human ovaries

Edwards¹,

Fowler²,

Gore-Langton³

et al. 1977

Reproduction

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“…In the present experiment, after 6 days of restraint LH concentrations were decreased (but not significantly) by 33% which corresponds as previously observed to the period of the gradual return toward normal of LH re¬ sponse to chronic stress. However, a fall in T without any changes in LH or even in presence of LH rise have also been reported (Ellis et al 1976;Nakasima et al 1975;Aono et al 1976 (Hafiez et al 1972;Grimek et al 1976;Zipf et al 1978a); however, it is doubtful that they play an important role in the decrease of steroidogenesis induced by immobilization stress. This result could confirm previous statements that adrenals are necessary for acquisition of adaptation to Stressors (Selye 1937 Gray et al (1978) have observed that in rats, chronic surgical stress induced a reduction in T levels accompanied in most cases by a decrement in LH values.…”

Section: Discussionmentioning

confidence: 97%

Effect of chronic intermittent immobilization stress on hypophyso-gonadal function of rats

Taché¹,

Ducharme²,

Charpenet³

et al. 1980

Acta Endocrinologica

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The neuroendocrine mechanisms involved in chronic stress-induced testosterone (T) suppression have been evaluated in adult male rats. In intact animals, restraint applied 6 h daily for 6 days increased plasma corticosterone, decreased significantly T and Prl, and, to a lesser degree, LH levels. In adrenalectomized (adr-x) rats exposed to the same stress regimen, plasma LH and T values were reduced, whereas Prl concentrations were significantly elevated as compared with intact stressed rats. Adrenalectomy alone did not modify basal levels of these hormones. In intact rats, chronic stress did not alter the amount of [125I]iodo-hCG specifically bound to crude testis membrane preparation, or the testicular responsiveness to hCG as measured by T levels 2 h after the injection of hCG (500 IU). In adr-x groups, a significant decrease in binding capacity of [125I]iodo-hCG and no alteration in hCG responsiveness have been observed. From these studies, it appears 1) that plasma T suppression induced by chronic restraint is not initially mediated by a direct action at the testis level as judged by the normal testicular hCG binding capacity and responsiveness to hCG, 2) the sustained endogenous release of

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“…The 0-13-0-32% (w/w) LH activities in bFSH-A and -B may be intrinsic LH-like activity, as described for ovine FSH (Grimek et al 1976). …”

Section: Sds-pagementioning

confidence: 78%

Isolation of FSH from bovine pituitary glands

Stanton²,

Robertson³

et al. 1993

Journal of Endocrinology

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An improved method is described for the isolation of FSH from bovine pituitary glands. The purification procedure consisted of an initial ammonium sulphate precipitation step followed by triazine-dye chromatography, immobilized metal affinity chromatography, high-performance anion-exchange chromatography and gel filtration. Three highly purified bovine FSH preparations (designated bFSH-A, -B and -C) were obtained, giving yields of approximately 5.7 mg FSH/kg bovine pituitary glands (wet weight), with specific radioreceptor activities for bFSH-A, -B and -C of 61, 25 and 29 units (NIH-FSH-S1)/mg protein respectively. The corresponding biological activities were 217 (bFSH-A), 62 (bFSH-B) and 86 (bFSH-C) units/mg, as measured by an FSH in-vitro bioassay. LH levels were found to be < 1% (w/w) as determined by an LH in-vitro bioassay. SDS-PAGE of these bFSH preparations under reducing conditions in 16% polyacrylamide gels showed two major silver-staining bands of apparent molecular masses 19.5 kDa and 15.8 kDa. Their amino acid compositions were in close agreement with the expected composition, based on the bFSH cDNA sequence and results reported by other investigators. N-terminal sequencing of the bFSH-A preparation yielded two major sequences consistent with alpha- and beta-subunits, and a third minor (< 20%) sequence consistent with the alpha-subunit clipped at amino acid residue 6. It was concluded that the bFSH purification procedure reported here is a rapid method which produces bFSH in high yield and high purity, with radioreceptor and in-vitro specific activities comparable with those previously reported by other investigators.

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Effect of Neuraminidase Treatment on the Biological Activity of Highly Purified Ovine FSH and LH n i Hypophysectomized Immature Male and Female Rats

Cited by 16 publications

References 0 publications

Normal and abnormal follicular growth in mouse, rat and human ovaries

Normal and abnormal follicular growth in mouse, rat and human ovaries

Effect of chronic intermittent immobilization stress on hypophyso-gonadal function of rats

Isolation of FSH from bovine pituitary glands

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