Effect of neurokinins on canine prostate cell physiology

Walden, Paul D.; Marinese, Dorene; Srinivasan, Dhanalakshmi; Tzoumaka, Elda; Syyong, Harley T.; Ford, Anthony; Bhattacharya, Anindya

doi:10.1002/pros.20195

Cited by 3 publications

(5 citation statements)

References 33 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensory neuropeptide calcitonin gene-related peptide (CGRP) has been found in nerves located within the stroma and/or glandular epithelium of numerous species 70 , 80 , 83 - 85 as well as neuroendocrine cells in the human prostate 86 . Functionally, exogenous CGRP has been shown to relax phenylephrine mediated contractions 78 or inhibit nerve mediated contractions 87 in the rat prostate.…”

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

“…Furthermore, tachykinins also modulate prostate contraction. Neurokinin A and substance P have been found distributed sparsely in nerve fibers supplying the prostatic smooth muscle of human, 50 sheep, 88 canine, 85 guinea pig, rat 89 , 90 and mouse prostates 70 . Exogenous application of tachykinin agonists induces prostate contraction via neurokinin NK2 receptors in the human prostate, 91 whereas neurokinin NK1 and NK2 receptors mediate contraction to exogenous agonists in cultured canine prostatic stromal cells 85 .…”

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

“…Neurokinin A and substance P have been found distributed sparsely in nerve fibers supplying the prostatic smooth muscle of human, 50 sheep, 88 canine, 85 guinea pig, rat 89 , 90 and mouse prostates 70 . Exogenous application of tachykinin agonists induces prostate contraction via neurokinin NK2 receptors in the human prostate, 91 whereas neurokinin NK1 and NK2 receptors mediate contraction to exogenous agonists in cultured canine prostatic stromal cells 85 . Conversly, no direct contractile effects have been observed in the rat 78 or pig 58 prostates in response to substance P. In the guinea pig prostate, however, substance P and neurokinin A potentiate contractions elictited by nerve stimulation 90 …”

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

See 2 more Smart Citations

Age-related changes in the innervation of the prostate gland

2013

View full text Add to dashboard Cite

The adult prostate gland grows and develops under hormonal control while its physiological functions are controlled by the autonomic nervous system. The prostate gland receives sympathetic input via the hypogastric nerve and parasympathetic input via the pelvic nerve. In addition, the hypogastric and pelvic nerves also provide sensory inputs to the gland. This review provides a summary of the innervation of the adult prostate gland and describes the changes which occur with age and disease. Growth and development of the prostate gland is age dependent as is the occurrence of both benign prostate disease and prostate cancer. In parallel, the activity and influence of both the sympathetic and parasympathetic nervous system changes with age. The influence of the sympathetic nervous system on benign prostatic hyperplasia is well documented and this review considers the possibility of a link between changes in autonomic innervation and prostate cancer progression.

show abstract

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

Section: Innervation Of the Adult Prostate Glandmentioning

confidence: 99%

See 1 more Smart Citation

Age-related changes in the innervation of the prostate gland

2013

View full text Add to dashboard Cite

show abstract

“…This is consistent with the hypothesis that sensory neuropeptides are implicated as significant regulators of prostate growth. Neurokinin 1, the receptor for substance P, has known effects on canine prostate epithelial cell proliferation, stromal differentiation, and contraction and neurokinin 1, neurokinin 2 and neurokinin 3 subtypes have been localized in canine prostate tissue by immunohistochemical analysis and ligand binding studies [22]. We have proposed that one component of bothersome LUTS is the activation of prostatic nociceptive pathways such that men complain of the bother regardless of the degree they are obstructed and independent of prostate size [23].…”

Section: Discussionmentioning

confidence: 99%

Effect of Onabotulinum Toxin A on Substance P and Receptor Neurokinin 1 in the Rat Ventral Prostate

et al. 2015

View full text Add to dashboard Cite

Introduction The objective of this work is to examine if sensory innervation impacts lower urinary tract symptoms (LUTS). Onabotulinum toxin A (BoNTA) has been used for the treatment of overactive and neurogenic bladder and as a treatment for LUTS secondary to benign prostatic hyperplasia (BPH). The mechanism of how BoNTA impacts LUTS/BPH is unclear. In rats, BoNTA injection causes prostate denervation, apoptosis and atrophy. In clinical trials reduced prostate size and LUTS are observed inconsistently, suggesting a neurologic component. We will examine if BoNTA treatment inhibits substance P production in sensory nerve fibers in the rat prostate. Methods Twenty Sprague Dawley rats were divided into four groups including 1X PBS (control, n=6), 2.5 units Onabotulinum toxin A (BoNTA, n=6), 5 units BoNTA (n=6) injected into both lobes of the ventral prostate (VP) and sham surgery (n=2). Rats were Euthanized after one week. Substance P and its receptor neurokinin 1 localization and quantification were performed by counting the number of stained neurons and nerve bundles, by semi-quantitative immunohistochemical analysis and by western analysis. Results Substance P was localized in neuronal axons and bundles in the stroma of the VP but not in the epithelium. Receptor neurokinin 1 was identified in neuronal bundles of the stroma and in columnar epithelium of the VP ducts. Substance P decreased ~90% after BoNTA treatment (p=0.0001) while receptor neurokinin 1 did not change by IHC (p=0.213) or Western (p=0.3675). Conclusions BoNTA treatment decreases substance P in the rat VP.

show abstract

“…Neurokinin and substance P have been sparsely localized in nerve fibers innervating the smooth muscle tunic of man, sheep, dog, guinea pig, rat, and mouse prostate glands ( 67 , 77 , 79 – 81 ). For galanin neuropeptide localization, sporadic GAL-positive nerve fibers were demonstrated in the prostate of hamster rats ( 82 ), while in the prostate of the Wistar rat, no GAL nerve fiber was demonstrated ( 83 ).…”

Section: Neuropeptidergic Outline Of the Male Accessory Glandsmentioning

confidence: 99%

Neuropeptide Profiles of Mammalian Male Genital Tract: Distribution and Functional Relevance in Reproduction

Omirinde

Azeez

2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Neuropeptides are secretory peptides characterized by small chains of amino acids linked by peptide bonds. They are majorly found in some mammalian neurons and glial cells, where they modulate a variety of physiological homeostasis. In the male genital tract, they are mostly found in the neuronal fibers supplying the vasculature, smooth muscle layer, interstitium, and lamina propria of the tunica mucosa of the various reproductive organs. Functionally, neuropeptides are strongly implicated in vascular temperature regulations, spermatozoa extrusion, epididymal content transportation, and movement of accessory gland secretions. This review provides an overview of neuropeptides with respect to their synthesis, release, and mechanism of actions, with emphasis on the locally acting neuropeptides, such as substance P (SP), neuropeptide Y (NPY), vasoactive intestinal peptides (VIP), calcitonin gene-related peptide (CGRP), galanin (GAL), cholecystokinin (CCK), C-terminal flanking peptide of NPY (CPON), peptide histidine isoleucine (PHI), and met- and leu-enkephalins (M-ENK and L-ENK) along the male genital tract (i.e., the spermatic cord, testis, epididymis, ductus deferens, and accessory sex organs) of 14 species of mammals and their marked influence on reproduction. This review also revealed from documented reports that the vast majority of neuropeptides present in the autonomic nerve supply to the male genital tract probably coexist with other peptides or with various neurotransmitters (tyrosine hydroxylase, dopamine beta hydroxylase, and 5-hydroxytryptamine). In addition, documented evidence of variation in age, season, and intraspecies differences were identified as notable factors of influence in peptidergic nerve fiber distribution.

show abstract

Effect of neurokinins on canine prostate cell physiology

Abstract: Our results suggest that, at least in vitro, neurokinins have modest effects on canine prostate epithelial cell proliferation, stromal differentiation, and contraction.

Cited by 3 publications

References 33 publications

Age-related changes in the innervation of the prostate gland

Age-related changes in the innervation of the prostate gland

Effect of Onabotulinum Toxin A on Substance P and Receptor Neurokinin 1 in the Rat Ventral Prostate

Neuropeptide Profiles of Mammalian Male Genital Tract: Distribution and Functional Relevance in Reproduction

Contact Info

Product

Resources

About