Effect of Neutralization of Gastric Aspirate on culture yield of mycobacterium tuberculosis in children with pulmonary tuberculosis

Anand, E R; Biswal, Niranjan; Joseph, Noyal Mariya

doi:10.1016/j.ijtb.2020.12.009

Cited by 2 publications

(1 citation statement)

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“…The advantages of such methods are that TB can be diagnosed once the etiological basis is found. However, there are also many disadvantages, including a low positive rate of etiology, high infectivity of specimens, and dependence on patients' subjective specimen retention (5,6). Researchers have applied tuberculosis antibody (TB-Ab) in the auxiliary diagnosis of TB, but its false positive rate is high and thus cannot provide clinicians certainty in the diagnosis of TB (7).…”

Section: Introductionmentioning

confidence: 99%

B and T lymphocyte attenuator as a C-reactive protein and IgA associated auxiliary diagnostic marker for pulmonary tuberculosis: a case-control study

Xu¹,

Bian²,

Wang³

et al. 2022

Ann Transl Med

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Background: Screening and identification of hematologic molecular indicators of pulmonary tuberculosis (PTB) is crucial for its diagnose and therapy. Therefore, our work aims to detect the diagnostic value of blood marker B and T lymphocyte attenuator (BTLA) in PTB, and provide a certain theoretical basis for the auxiliary diagnosis of PTB. Methods: Based on the inclusion criteria, 56 Patients with clinically confirmed pulmonary TB by clinical between January 2020 and December 2021 at our hospital were selected as the research objects of this study. Fifty-two matched healthy population at our hospital was used as the control group. Clinical characteristics were got from clinical laboratory. Real-time polymerase chain reaction (RT-PCR) was used to analyze changes in BTLA along with its ligand in peripheral blood. Changes in BTLA on the surface of different cells were analyzed by flow cytometry. The correlation test was used to determine the associations between BTLA and clinical indicators. Receiver operating characteristic (ROC) curve analysis was used to evaluate the auxiliary diagnostic value in PTB of BTLA expression from different sources.Results: Compared with the control, changes in peripheral blood BTLA in the PTB group were significantly increased (P=0.0187) rather than its ligand. Changes in BTLA on the surface of CD68 and antigen-presenting cell (APC) CD11c were significantly increased in the PTB group (P=0.0004, P<0.0001), while changes in BTLA on the surface of CD4 + T and CD8 + T cells were not significantly different (P=0.0792, P=0.8706). The expression of BTLA + CD11c + was negatively correlated with the expression of immunoglobulin A (IgA) (r=−0.2934, P=0.0282) and positively related to C-reactive protein (r=0.3277, P=0.0137). ROC curve analysis suggested that the area under the curve (AUC), sensitivity and specificity of BTLA RT-PCR detection were 0.6315, 53.57%, 57.69% while for BTLA + CD11c + detection were 0.8039, 88.46% and 73.21% and for BTLA + CD68 + detection were 0.6973, 60.71% and 61.54%.Conclusions: BTLA is highly expressed in peripheral blood and specific cell types of patients with PTB and is correlated with specific clinical indicators, which may be an important molecular marker for the auxiliary diagnosis of PTB.

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Section: Introductionmentioning

confidence: 99%